Library Preparation & Sequencing Request Form | Microarray & Sequencing Resource

Sample QC via nanodrop and bioanalyzer is performed as part of library preparation. Complete ONLY this form if you would like the BUMSR Core to prepare libraries for your samples followed by sequencing. DO NOT complete the Bioanalyzer QC form or the Sequencing form in addition.

Hidden

Date

Contact

Submitter Name(Required)

First Last

Submitter Email(Required)

Submitter Telephone No.(Required)

P. I. Name(Required)

First Last

P. I. Email(Required)

Affiliation(Required)

Department(Required)

Address(Required)

Street Address Address Line 2 City State ZIP Code

Accounting

Do you have a BU Grant Account Number?(Required)

Please email the MSR Core at MSRLab@bu.edu with your account number as soon as you are able to provide one. The service will not be provided until the Core receives the account number.

Do you have an Invoice Number?(Required)

Please email the MSR Core at MSRLab@bu.edu if you do not have an invoice number. The service will not be provided until the Core generates an invoice.

Grant Administrator Name(Required)

First Last

Grant Administrator Email(Required)

Sequencing Library Preparation

Number of Samples(Required)

Sample Species(Required)

Human

Mouse

Other

Library Preparation Type(Required)

NEBNext UltraExpress mRNA with Poly-A Selection

Illumina mRNA with Poly-A Selection

NEBNext UltraExpress Total RNA with rRNA Reduction

Illumina Total RNA with rRNA Reduction

NEBNext Low Input/ Single Cell RNA

EpiCypher CUT & RUN

Illumina Nextera XT

Illumina DNA Prep with Exome 2.5 Enrichment

How did you isolate your RNA?(Required)

Please indicate the exact name of the RNA Isolation Kit you used to prepare your samples. If you did not use a kit, please specify your process and place additional details in the "Final Notes" section of this form.

Did you perform DNAse digestion (or similar process) during RNA isolation to remove genomic DNA?(Required)

Number of Libraries to be Prepared(Required)

Investigators may submit more samples than planned for library preparation (e.g., choose highest quality samples after initial QC). Please indicate the total number of libraries to prepare.

Sample Volume and Concentration Information(Required)

Please download and fill out the QC sample information excel sheet here before uploading it above. Sample names should NOT EXCEED 5 alpha-numeric characters. Please include all information requested or indicate "TBD" if unknown.

Max. file size: 100 MB.

Illumina Sequencing

Sequencing Output(Required)

NOTE: The MSR Core has switched to Illumina's NEW XLEAP chemistry. If you would like your libraries sequenced using Old v3 chemistry, please indicate that in the Final Notes field at the end of this form.

NextSeq 2000 - P1 - 100M Reads

NextSeq 2000 - P2 - 400M Reads

NextSeq 2000 - P3 - 1.1B Reads

NextSeq 2000 - P4 - 1.7B Reads

NextSeq 500 - Mid - 100M Reads

NextSeq 500 - High - 400M Reads

NextSeq 2000 P1 Read Length(Required)

P1-100: 100 bp - Single Read

P1-100: 50 bp - Paired End Reads

P1-300: 300 bp - Single Read

P1-300: 150 bp - Paired End Reads

P1-600: 300 bp - Paired End Reads

Other

NextSeq 2000 P2 Read Length(Required)

P2-100: 100bp - Single Reads

P2-100: 50bp - Paired End Reads

P2-200: 200 bp - Single Read

P2-200: 100 bp - Paired End Reads

P2-300: 300 bp - Single Read

P2-300: 150 bp - Paired End Reads

P2-600: 300 bp - Paired End Reads

Other

NextSeq 2000 P3 Read Length(Required)

P3-50: 50 bp - Single Reads

P3-100: 100bp - Single Reads

P3-100: 50bp - Paired End Reads

P3-200: 200 bp - Single Read

P3-200: 100 bp - Paired End Reads

P3-300: 300 bp - Single Read

P3-300: 150 bp - Paired End Reads

Other

NextSeq 2000 P4 Read Length(Required)

P4-50: 50 bp - Single Reads

P4-100: 100bp - Single Reads

P4-100: 50bp - Paired End Reads

P4-200: 200 bp - Single Read

P4-200: 100 bp - Paired End Reads

P4-300: 300 bp - Single Read

P4-300: 150 bp - Paired End Reads

Other

NextSeq 500 Mid Read Length(Required)

Mid-150: 150 bp - Single Reads

Mid-150: 75bp - Paired End Reads

Mid-300: 300 bp - Single Read

Mid-300: 150 bp - Paired End Reads

Other

NextSeq 500 High Read Length(Required)

High-75: 75bp - Single Reads

High-150: 150 bp - Single Reads

High-150: 75bp - Paired End Reads

High-300: 300 bp - Single Read

High-300: 150 bp - Paired End Reads

Other

Number of Sequencing Runs(Required)

Please indicate the number of sequencing runs you would like performed to achieve desired coverage.

Would you like the remainder of unused sample returned to you with libraries after sequencing completion?

If you request sample return, the BUMSR Core will contact you to schedule retrieval or return shipping after QC data delivery.

Analysis

Do you need analysis from the BUMSR Computational Biology team?(Required)

Basic RNA-Seq analysis is available at additional cost to the sequencing run.

The BUMSR Core uses Illumina’s BaseSpace cloud platform to store and transfer sequencing data. If you (or the analyst assigned to your study) have not previously, please sign up for a free BaseSpace account at here and provide the email used to register below.

What is the email associated with the BaseSpace account designated to receive raw sequencing data for storage and/or downstream analysis?

This can be an analyst assigned to the project or another investigator

Final Notes