10X Spatial Sequencing Parameters

Assay Name	Sample Type	Slide Type	Minimum No. of Sequencing Reads Needed¹
			(per 100% Coverage of Capture Area)
Visium HD Spatial Gene Expression²	Fresh-Frozen	6.5 mm	700 M
	Fixed-Frozen	6.5 mm	500 M
	FFPE	6.5 mm	275 M
Visium HD Spatial Gene Expression³	Fresh-Frozen	11.0 mm	2.1 B
	Fixed-Frozen	11.0 mm	1.5 B
	FFPE	11.0 mm	825 M
Visium HD 3′ Spatial Gene Expression⁴	Fresh-Frozen	6.5 mm	550 M
Visium CytAssist Spatial Gene Expression⁵	Any	6.5 mm	125 M
	Any	11.0 mm	375.5 M

^{1. To determine minimum number of reads needed per sample, multiply the percent of capture area covered with the sample tissue by the number of reads needed if 100% of the capture area is covered for your tissue type. E.g., Tissue Sample A was fresh-frozen and covered 15% of the capture area of a Visium HD Spatial 6.5mm slide. Therefore minimum reads needed = 0.15 x 700,000,000 or 105,000,000 (105 M).}

^{2. See 10X Genomics protocol CG000685 – Visium HD Spatial Gene Expression Reagent Kits}

^{3. See 10X Genomics protocol CG001679 – Visium HD Spatial Gene Expression 2.0}

^{4. See 10X Genomics protocol CG000805 – Visium HD 3′ Spatial Gene Expression}

^{5. See 10X Genomics protocol CG000494 – Visium CytAssist Spatial Gene and Protein Expression Reagent Kits}