{"id":14,"date":"2017-11-09T15:21:20","date_gmt":"2017-11-09T20:21:20","guid":{"rendered":"https:\/\/www.bumc.bu.edu\/singlecell\/?page_id=14"},"modified":"2021-03-29T13:44:32","modified_gmt":"2021-03-29T17:44:32","slug":"services","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/singlecell\/services\/","title":{"rendered":"Services"},"content":{"rendered":"

As a high-throughput core, we provide an array of services to get you from sample to high-quality single cell gene expression data and\/or analysis. We offer multiple single cell library preparation options and next-generation Illumina sequencing via our collaboration with the BU Microarray & Sequencing Core. Uncover critical insight with our services.<\/p>\n

Single Cell Services<\/h2>\n

10X Genomics Chromium\u2122 System<\/h4>\n

10x Genomics’<\/a> Chromium System is a unique system that partitions cells and prepares sequencing libraries in parallel such that all fragments produced within a partition share a common barcode.
\n\"10x_SingleCell_Chromium\"
\nSpecifically, The Chromium Single Cell 3\u2019 Solution<\/a> provides a comprehensive, scalable solution via high-throughput single cell characterization and gene expression profiling. This enables novel discovery of gene expression dynamics and molecular profiling of individual cell types in an affordable and simple workflow.\u00a0 10X Chromium Single Cell solutions can capture between 500 – 10,000 cells from samples with cell concentrations of 100 – 2000 cells\/uL.<\/p>\n

Illumina NextSeq NGS Sequencing<\/h4>\n

We collaborate with the Botson University Microarray & Sequencing Core to provide you with the best sequencing for your library via\u00a0Illumina<\/a>\u00a0NextSeq 500 and 2000 platforms.<\/p>\n

Data Analysis<\/h4>\n

We offer detailed analysis of your single cell data. For further details and pricing, please contact us<\/a>.
\n\"analytics-iMac_1920\"<\/p>\n

Tissue Dissociation Services<\/h2>\n

As part of our mission to provide high quality single cell sequencing data, we offer walk-up access to our Miltenyi Biotech GentleMACS Octo tissue dissociator with heaters. By appointment, researchers may utilize our GentleMACS instrument to develop fully automated protocols for dissociating their tissues of interest into cell suspensions ready for downstream applications.<\/p>\n

Miltenyi Biotec GentleMACS Octo Tissue Dissociator<\/h4>\n

The Miltenyi Biotec GentleMACS\u2122 Octo Dissociator with Heaters<\/a> is a benchtop instrument for the fully automated and standardized tissue dissociation or homogenization of up to eight samples.\u00a0<\/span><\/p>\n

\"GentleMACS<\/p>\n

Equipped with eight individual heating units and ready-to-use gentleMACS programs, it offers a fully-automated workflow for tissue dissociation. It also allows for the creation of user-defined programs. \u00a0All eight positions can be operated independently.<\/p>\n

Single-cell suspensions or thorough homogenates are obtained using single-use, sterile C Tubes or M Tubes. \u00a0C tubes and M tubes may be obtained through Miltenyi Biotech<\/a>.<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

As a high-throughput core, we provide an array of services to get you from sample to high-quality single cell gene expression data and\/or analysis. We offer multiple single cell library preparation options and next-generation Illumina sequencing via our collaboration with the BU Microarray & Sequencing Core. Uncover critical insight with our services. Single Cell Services […]<\/p>\n","protected":false},"author":14138,"featured_media":0,"parent":0,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/singlecell\/wp-json\/wp\/v2\/pages\/14"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/singlecell\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/singlecell\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/singlecell\/wp-json\/wp\/v2\/users\/14138"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/singlecell\/wp-json\/wp\/v2\/comments?post=14"}],"version-history":[{"count":46,"href":"https:\/\/www.bumc.bu.edu\/singlecell\/wp-json\/wp\/v2\/pages\/14\/revisions"}],"predecessor-version":[{"id":555,"href":"https:\/\/www.bumc.bu.edu\/singlecell\/wp-json\/wp\/v2\/pages\/14\/revisions\/555"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/singlecell\/wp-json\/wp\/v2\/media?parent=14"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}