{"id":47,"date":"2010-04-24T07:39:11","date_gmt":"2010-04-24T11:39:11","guid":{"rendered":"https:\/\/www.bumc.bu.edu\/ravidlab\/?page_id=47"},"modified":"2023-06-22T22:09:56","modified_gmt":"2023-06-23T02:09:56","slug":"available-laboratory-protocols","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/ravidlab\/available-laboratory-protocols\/","title":{"rendered":"Selected List of Available Laboratory Protocols"},"content":{"rendered":"<p>(<a href=\"mailto:remac@bu.edu\">Upon Inquiry<\/a>)<\/p>\n<p>Preparation of murine bone marrow (and megakaryocyte) cultures<\/p>\n<p>Purification of megakaryocytes (gradient-based or by antibody affinity column)<\/p>\n<p>Live Imaging of megakaryocytes<\/p>\n<p>Transfection of primary bone marrow cells<\/p>\n<p>EMSA and foot printing<\/p>\n<p>Blood counts and blood clotting assays<\/p>\n<p>Ploidy determination by Flow Cytometry<\/p>\n<p>FACS Analysis of bone marrow progenitors<\/p>\n<p>Immunohistochemistry<\/p>\n<p>Generation of transgenic constructs<\/p>\n<p>Generation of Knockout constructs<\/p>\n<p>Generation of transgenic and knockout lines<\/p>\n<p>Preparation of mouse or rat aortic vascular smooth muscle cell cultures<\/p>\n<p>Femoral artery injury Model<\/p>\n<p>High Fat Diet induced Atherosclerosis<\/p>\n<p>Cryosection and immunohistochemistry<\/p>\n<p>Mesenchymal stem cell cultures<\/p>\n<p>Differentiation of Bone Marrow Mesenchymal Stem Cells<\/p>\n<p><strong>Figure<\/strong>: \u03b2-Gal staining of different organs and cells derived from WT or A2b adenosine receptor knockout, \u00df-gal knock-in mice (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC1483170\/\">The A2B adenosine receptor protects against inflammation and excessive vascular adhesion<\/a>. Yang D, Zhang Y, Nguyen HG, Koupenova M, Chauhan AK, Makitalo M, Jones MR, St Hilaire C, Seldin DC, Toselli P, Lamperti E, Schreiber BM, Gavras H, Wagner DD, Ravid K. J Clin Invest. 2006 Jul;116(7):1913-23.)<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" src=\"\/ravidlab\/files\/2023\/06\/ravid3-protocol-page-636x531.jpg\" alt=\"\" class=\"alignnone size-medium wp-image-1099\" width=\"636\" height=\"531\" srcset=\"https:\/\/www.bumc.bu.edu\/ravidlab\/files\/2023\/06\/ravid3-protocol-page-636x531.jpg 636w, https:\/\/www.bumc.bu.edu\/ravidlab\/files\/2023\/06\/ravid3-protocol-page-768x641.jpg 768w, https:\/\/www.bumc.bu.edu\/ravidlab\/files\/2023\/06\/ravid3-protocol-page.jpg 934w\" sizes=\"(max-width: 636px) 100vw, 636px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>(Upon Inquiry) Preparation of murine bone marrow (and megakaryocyte) cultures Purification of megakaryocytes (gradient-based or by antibody affinity column) Live Imaging of megakaryocytes Transfection of primary bone marrow cells EMSA and foot printing Blood counts and blood clotting assays Ploidy determination by Flow Cytometry FACS Analysis of bone marrow progenitors Immunohistochemistry Generation of transgenic constructs [&hellip;]<\/p>\n","protected":false},"author":1249,"featured_media":0,"parent":0,"menu_order":9,"comment_status":"closed","ping_status":"open","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/ravidlab\/wp-json\/wp\/v2\/pages\/47"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/ravidlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/ravidlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/ravidlab\/wp-json\/wp\/v2\/users\/1249"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/ravidlab\/wp-json\/wp\/v2\/comments?post=47"}],"version-history":[{"count":14,"href":"https:\/\/www.bumc.bu.edu\/ravidlab\/wp-json\/wp\/v2\/pages\/47\/revisions"}],"predecessor-version":[{"id":1100,"href":"https:\/\/www.bumc.bu.edu\/ravidlab\/wp-json\/wp\/v2\/pages\/47\/revisions\/1100"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/ravidlab\/wp-json\/wp\/v2\/media?parent=47"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}