{"id":20996,"date":"2023-06-05T13:50:51","date_gmt":"2023-06-05T17:50:51","guid":{"rendered":"http:\/\/www.bumc.bu.edu\/ppb\/?post_type=profile&#038;p=20996"},"modified":"2023-07-12T11:18:30","modified_gmt":"2023-07-12T15:18:30","slug":"g-graham-shipley","status":"publish","type":"profile","link":"https:\/\/www.bumc.bu.edu\/ppb\/profile\/g-graham-shipley\/","title":{"rendered":"G. Graham Shipley"},"content":{"rendered":"<h4><a id=\"research\" name=\"research\"><\/a>Research<\/h4>\n<h5>Membrane and Receptor Biology<\/h5>\n<p><\/a>Our interests center around the structure and function of cell membranes, receptor-ligand interactions and transmembrane signaling mechanisms. A combination of biochemical, chemical and biophysical approaches are used to probe: (1) membrane lipid conformation, structure, properties and interactions, (2) membrane receptor-lipid interactions and (3) membrane receptor-ligand interactions. Current approaches involve the isolation of membrane-associated proteins and lipids (e.g. low density lipoprotein (LDL) receptor, insulin receptor, integrins, gangliosides), their reconstitution with well characterized lipid monolayer and bilayer matrices and structural studies of the protein-lipid assemblies. Ligand binding studes include LDL receptor\/LDL, insulin receptor\/insulin, and ganglioside\/toxin interactions. The biochemical methods involved include lipid and protein isolation, gel\/affinity chromatography, antibody techniques, immunoblotting, detergent solubilization, lipid-protein reconstitution, etc. Chemical methods include lipid sythesis, TLC, HPLC, IR and NMR. The biophysical approach involves x-ray diffraction, protein crystallography, electron microscopy\/image reconstruction, surface chemistry and calorimetric and spectroscopic (CD, NMR) methods.<\/p>\n<h5>Links:<\/h5>\n<p><a href=\"https:\/\/www.bumc.bu.edu\/busm\/profile\/g-shipley\/\">Faculty Profile<\/a><br \/>\n<a href=\"https:\/\/www.researchgate.net\/scientific-contributions\/G-G-Shipley-38514070\">ResearchGate<\/a><br \/>\n<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=shipley%2C+gg%5Bauthor%5D\">PubMed<\/a><\/p>\n","protected":false},"author":1811,"template":"","_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/profile\/20996"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/profile"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/types\/profile"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/users\/1811"}],"version-history":[{"count":4,"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/profile\/20996\/revisions"}],"predecessor-version":[{"id":21425,"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/profile\/20996\/revisions\/21425"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/media?parent=20996"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}