{"id":22735,"date":"2024-01-16T09:25:41","date_gmt":"2024-01-16T14:25:41","guid":{"rendered":"https:\/\/www.bumc.bu.edu\/ppb\/?page_id=22735"},"modified":"2024-02-07T09:14:15","modified_gmt":"2024-02-07T14:14:15","slug":"classic-npcs","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/ppb\/classic-npcs\/","title":{"rendered":""},"content":{"rendered":"<h4>Classic NPCs<\/h4>\n<p>&nbsp;<\/p>\n<figure id=\"attachment_22730\" aria-describedby=\"caption-attachment-22730\" style=\"width: 810px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" src=\"\/ppb\/files\/2024\/01\/Necturus-dform-NPCs-lamina-1024x659.jpg\" alt=\"\" width=\"800\" height=\"515\" class=\"wp-image-22730\" srcset=\"https:\/\/www.bumc.bu.edu\/ppb\/files\/2024\/01\/Necturus-dform-NPCs-lamina-1024x659.jpg 1024w, https:\/\/www.bumc.bu.edu\/ppb\/files\/2024\/01\/Necturus-dform-NPCs-lamina-466x300.jpg 466w, https:\/\/www.bumc.bu.edu\/ppb\/files\/2024\/01\/Necturus-dform-NPCs-lamina-768x495.jpg 768w, https:\/\/www.bumc.bu.edu\/ppb\/files\/2024\/01\/Necturus-dform-NPCs-lamina-1536x989.jpg 1536w, https:\/\/www.bumc.bu.edu\/ppb\/files\/2024\/01\/Necturus-dform-NPCs-lamina.jpg 2019w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><figcaption id=\"caption-attachment-22730\" class=\"wp-caption-text\">Cryogenic-EM image of detergent extracted <em>Necturus<\/em> nuclear envelope with NPCs arrayed in local patches as square arrays due to their interactions with the ordered lamina. [Akey, 1989]<\/figcaption><\/figure>\n<figure id=\"attachment_22731\" aria-describedby=\"caption-attachment-22731\" style=\"width: 810px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" src=\"\/ppb\/files\/2024\/01\/Picture1-mform-vNPCs-2-1024x782.jpg\" alt=\"\" width=\"800\" height=\"611\" class=\"wp-image-22731\" srcset=\"https:\/\/www.bumc.bu.edu\/ppb\/files\/2024\/01\/Picture1-mform-vNPCs-2-1024x782.jpg 1024w, https:\/\/www.bumc.bu.edu\/ppb\/files\/2024\/01\/Picture1-mform-vNPCs-2-393x300.jpg 393w, https:\/\/www.bumc.bu.edu\/ppb\/files\/2024\/01\/Picture1-mform-vNPCs-2-768x587.jpg 768w, https:\/\/www.bumc.bu.edu\/ppb\/files\/2024\/01\/Picture1-mform-vNPCs-2.jpg 1508w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><figcaption id=\"caption-attachment-22731\" class=\"wp-caption-text\">Cryogenic-EM image: spread <em>Xenopus<\/em> nuclear envelope with NPCs (high density is black) that exhibit ring-like features in the central channel (black rings) of virtually every particle. [Akey &amp; Radermacher, 1993]<\/figcaption><\/figure>\n<figure id=\"attachment_22732\" aria-describedby=\"caption-attachment-22732\" style=\"width: 610px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" src=\"\/ppb\/files\/2024\/01\/vNPCs-3-1024x821.jpg\" alt=\"\" width=\"600\" height=\"481\" class=\"wp-image-22732\" srcset=\"https:\/\/www.bumc.bu.edu\/ppb\/files\/2024\/01\/vNPCs-3-1024x821.jpg 1024w, https:\/\/www.bumc.bu.edu\/ppb\/files\/2024\/01\/vNPCs-3-374x300.jpg 374w, https:\/\/www.bumc.bu.edu\/ppb\/files\/2024\/01\/vNPCs-3-768x615.jpg 768w, https:\/\/www.bumc.bu.edu\/ppb\/files\/2024\/01\/vNPCs-3.jpg 1218w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><figcaption id=\"caption-attachment-22732\" class=\"wp-caption-text\">(top left) 2D average of amphibian NPCs that show a ring-like density in the central channel. (top right, bottom) A cylindrical structure in the central channel of Xenopus NPCs, named the central transporter (in pink),\u00a0 is now understood to be comprised of FG-repeat containing Nups and transport factors. [Akey, 1990; Akey &amp; Radermacher, 1993]<\/figcaption><\/figure>\n<h5>Selected Publications:<br \/>\n<strong><\/strong><\/h5>\n<p><strong>Akey CW<\/strong>, Radermacher M. (1993). Architecture of the <em>Xenopus<\/em> nuclear pore complex revealed by three-dimensional cryo-electron microscopy. J Cell Biol. 122:1-19. doi: 10.1083\/jcb.122.1.1. PMID: 8314837; PMCID: PMC2119598.<\/p>\n<p><strong>Akey CW<\/strong>. Visualization of transport-related configurations of the nuclear pore transporter. (1990). Biophys J. 58:341-355. doi: 10.1016\/S0006-3495(90)82381-X. PMID: 2207242; PMCID: PMC1280976.<\/p>\n<p><strong>Akey CW<\/strong>. Interactions and structure of the nuclear pore complex revealed by cryo-electron microscopy. (1989). J Cell Biol. 109:955-970. doi: 10.1083\/jcb.109.3.955. PMID: 2768344; PMCID: PMC2115778.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Classic NPCs &nbsp; &nbsp; &nbsp; Selected Publications: Akey CW, Radermacher M. (1993). Architecture of the Xenopus nuclear pore complex revealed by three-dimensional cryo-electron microscopy. J Cell Biol. 122:1-19. doi: 10.1083\/jcb.122.1.1. PMID: 8314837; PMCID: PMC2119598. Akey CW. Visualization of transport-related configurations of the nuclear pore transporter. (1990). Biophys J. 58:341-355. doi: 10.1016\/S0006-3495(90)82381-X. PMID: 2207242; PMCID: PMC1280976. [&hellip;]<\/p>\n","protected":false},"author":1811,"featured_media":0,"parent":0,"menu_order":19,"comment_status":"closed","ping_status":"closed","template":"page-templates\/no-sidebars.php","meta":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/pages\/22735"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/users\/1811"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/comments?post=22735"}],"version-history":[{"count":11,"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/pages\/22735\/revisions"}],"predecessor-version":[{"id":22818,"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/pages\/22735\/revisions\/22818"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/media?parent=22735"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}