{"id":21657,"date":"2023-09-14T10:48:34","date_gmt":"2023-09-14T14:48:34","guid":{"rendered":"http:\/\/www.bumc.bu.edu\/ppb\/?page_id=21657"},"modified":"2023-12-04T11:21:25","modified_gmt":"2023-12-04T16:21:25","slug":"the-mcknight-lab","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/ppb\/the-mcknight-lab\/","title":{"rendered":"The McKnight Lab"},"content":{"rendered":"

Director, BUSM Core Facility for Structural NMR<\/a><\/h4>\n

<\/a>Research<\/h4>\n
\"\"
The 35-residue villin headpiece subdomain, HP35. The sidechains of buried hydrophobic residues are shown as spheres. Other side chains are shown as sticks and colored by atom.<\/strong><\/figcaption><\/figure>\n
<\/h5>\n


The McKnight lab is interested in the folding, function and dynamics of proteins. A long-time favorite model system is the actin-binding \u201cheadpiece\u201d domain of the protein villin. The villin headpiece contains a subdomain of only 35-residues that folds to form a novel three helix structure (HP35). HP35 is one of the shortest amino acid sequences that folds to a monomeric native state in the absence of disulfide bonds or bound metals\/ligands. We use NMR, circular dichroism spectroscopy, and other methods, to investigate the stability and motions of proteins using the unique HP35 domain as well as other small protein domains.<\/p>\n

<\/a>Recent Publications<\/strong><\/h4>\n

Full list of publications on PubMed<\/a><\/p>\n

Vugmeyster, L., Rodgers, A, Ostrovsky, D, McKnight, C.J. & Fu, R. Deuteron off-resonance rotating frame relaxation for the characterization of slow motions in rotating and static solid-state proteins, Journal of Magnetic Resonance. 2023. 352, 107493:1-13. PMID: 37271094<\/p>\n

Vugmeyster L, Nichols PJ, Ostrovsky D, McKnight CJ, V\u00f6geli B, Slow methyl axes motions in perdeuterated villin headpiece subdomain probed by cross-correlated NMR relaxation measurements. Magnetochemistry. 2023. 9, 33:1-15. PMC9910280<\/p>\n

Nichols PJ, Falconer I, Griffin A, Mant C, Hodges R, McKnight CJ, V\u00f6geli B, Vugmeyster L. Deuteration of nonexchangeable protons on proteins affects their thermal stability, side-chain dynamics, and hydrophobicity. Protein Sci. 2020 07; 29(7):1641-1654. PMID: 32356390.<\/p>\n

Vugmeyster L, Griffin A, Ostrovsky D, Bhattacharya S, Nichols PJ, McKnight CJ, V\u00f6geli B. Correlated motions of C’-N and Ca-C\u00df pairs in protonated and per-deuterated GB3. J Biomol NMR. 2018 Oct; 72(1-2):39-54. PMID: 30121872.<\/p>\n

Miears HL, Gruber DR, Horvath NM, Antos JM, Young J, Sigurjonsson JP, Klem ML, Rosenkranz EA, Okon M, McKnight CJ, Vugmeyster L, Smirnov SL. Plant Villin Headpiece Domain Demonstrates a Novel Surface Charge Pattern and High Affinity for F-Actin. Biochemistry. 2018 03 20; 57(11):1690-1701. PMID: 29444403.<\/p>\n

Xiao R, Dane EL, Zeng J, McKnight CJ, Grinstaff MW. Synthesis of Altrose Poly-amido-saccharides with \u00df-N-(1>2)-d-amide Linkages: A Right-Handed Helical Conformation Engineered in at the Monomer Level. J Am Chem Soc. 2017 10 11; 139(40):14217-14223. PMID: 28902504.<\/p>\n

Falconer IB, Mant CT, McKnight CJ, Vugmeyster L, Hodges R. Optimized purification of a fusion protein by reversed-phase high performance liquid chromatography informed by the linear solvent strength model. J Chromatogr A. 2017 Oct 27; 1521:44-52. PMID: 28942999.<\/p>\n

Chin SL, Lu Q, Dane EL, Dominguez L, McKnight CJ, Straub JE, Grinstaff MW. Combined Molecular Dynamics Simulations and Experimental Studies of the Structure and Dynamics of Poly-Amido-Saccharides. J Am Chem Soc. 2016 05 25; 138(20):6532-40. PMID: 27119983.<\/p>\n

Harpole KW, O’Brien ES, Clark MA, McKnight CJ, Vugmeyster L, Wand AJ. The unusual internal motion of the villin headpiece subdomain. Protein Sci. 2016 Feb; 25(2):423-32. PMID: 26473993.<\/p>\n

Raina D, Agarwal P, Lee J, Bharti A, McKnight CJ, Sharma P, Kharbanda S, Kufe D. Characterization of the MUC1-C Cytoplasmic Domain as a Cancer Target. PLoS One. 2015; 10(8):e0135156. PMID: 26267657.<\/p>\n

Chen L, Brown JW, Mok YF, Hatters DM, McKnight CJ. The allosteric mechanism induced by protein kinase A (PKA) phosphorylation of dematin (band 4.9). J Biol Chem. 2015 Jul 17; 290(29):17808. PMID: 26188045.<\/p>\n

Brown JW, Bullitt E, Sriswasdi S, Harper S, Speicher DW, McKnight CJ. The Physiological Molecular Shape of Spectrin: A Compact Supercoil Resembling a Chinese Finger Trap. PLoS Comput Biol. 2015 Jun; 11(6):e1004302. PMID: 26067675.<\/p>\n

Links<\/h4>\n

BU Profile<\/a>
\nResearchGate<\/a>
\nLinkedIn<\/a>
\nFacility for Structural NMR<\/a><\/p>\n

<\/a>Contact Us<\/h4>\n

Department of Pharmacology, Physiology & Biophysics
\nChobanian & Avedisian School of Medicine
\n72 East Concord Street, L317
\nBoston MA 02118-2526<\/p>\n

Phone: (617) 358-9553
\ne-mail: cjmck@bu.edu<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Director, BUSM Core Facility for Structural NMR Research The McKnight lab is interested in the folding, function and dynamics of proteins. A long-time favorite model system is the actin-binding \u201cheadpiece\u201d domain of the protein villin. The villin headpiece contains a subdomain of only 35-residues that folds to form a novel three helix structure (HP35). HP35 […]<\/p>\n","protected":false},"author":1811,"featured_media":0,"parent":0,"menu_order":47,"comment_status":"closed","ping_status":"closed","template":"page-templates\/no-sidebars.php","meta":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/pages\/21657"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/users\/1811"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/comments?post=21657"}],"version-history":[{"count":7,"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/pages\/21657\/revisions"}],"predecessor-version":[{"id":22539,"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/pages\/21657\/revisions\/22539"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/ppb\/wp-json\/wp\/v2\/media?parent=21657"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}