{"id":2596,"date":"2016-08-03T16:32:36","date_gmt":"2016-08-03T20:32:36","guid":{"rendered":"https:\/\/www.bumc.bu.edu\/msr\/?page_id=2596"},"modified":"2017-10-11T14:34:16","modified_gmt":"2017-10-11T18:34:16","slug":"integrated-omics-from-tissue-slides","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/msr\/research-topics\/integrated-omics-from-tissue-slides\/","title":{"rendered":"Integrated omics from tissue slides"},"content":{"rendered":"<figure id=\"attachment2598\" aria-describedby=\"caption-attachment2598\" style=\"width: 410px\" class=\"wp-caption alignright\"><img loading=\"lazy\" src=\"\/msr\/files\/2016\/08\/160803-GAG-ECM-400x300.jpg\" alt=\"160803 GAG ECM\" class=\"wp-image-2598 size-medium\" height=\"300\" width=\"400\" srcset=\"https:\/\/www.bumc.bu.edu\/msr\/files\/2016\/08\/160803-GAG-ECM-400x300.jpg 400w, https:\/\/www.bumc.bu.edu\/msr\/files\/2016\/08\/160803-GAG-ECM.jpg 960w\" sizes=\"(max-width: 400px) 100vw, 400px\" \/><figcaption id=\"caption-attachment2598\" class=\"wp-caption-text\">The interplay of cell surface and extracellular glycoconjugates modulates accessibility of growth factors to cell surface signaling receptors.<\/figcaption><\/figure>\n<p>Cell surface and extracellular matrix glycoproteins and proteoglycans modulate the accessibility of growth factor ligands to cell surface receptors, thereby influencing signalling transduction.\u00a0 It is therefore not surprising that dysregulation of homeostasis of cell surface and extracellular matrix molecules is a hallmark of neurological diseases and cancers.\u00a0 We developed a method for printing glycosidase or protease enzymes to tissue slide surfaces, extraction of the digestion products and LC-MS analysis.<\/p>\n<ul>\n<li>Shao, C.; Shi, X.; Phillips, J. J.; Zaia, J. Mass spectral profiling of glycosaminoglycans from histological tissue surfaces. <em>Anal. Chem.<\/em> <strong>2013<\/strong>, <em>85<\/em>, 10984-10991.<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24099043\" target=\"blank\"><span style=\"color: #3300ff;\" color=\"#3300ff\"> Pubmed Link<\/span><\/a><\/li>\n<li>Turi\u00e1k, L.; Shao, C.; Meng, L.; Khatri, K.; Leymarie, N.;Wang, Q.; Pantazopoulos, H.; Leon,\n<figure id=\"attachment2599\" aria-describedby=\"caption-attachment2599\" style=\"width: 410px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" src=\"\/msr\/files\/2016\/08\/160803-Integrated-omics-of-TMAs-400x300.jpg\" alt=\"160803 Integrated omics of TMAs\" class=\"wp-image-2599 size-medium\" height=\"300\" width=\"400\" srcset=\"https:\/\/www.bumc.bu.edu\/msr\/files\/2016\/08\/160803-Integrated-omics-of-TMAs-400x300.jpg 400w, https:\/\/www.bumc.bu.edu\/msr\/files\/2016\/08\/160803-Integrated-omics-of-TMAs.jpg 960w\" sizes=\"(max-width: 400px) 100vw, 400px\" \/><figcaption id=\"caption-attachment2599\" class=\"wp-caption-text\">A work flow for recovering glycans and proteins<\/figcaption><\/figure>\n<p>D. R.; Zaia, J. Workflow for Combined Proteomics and Glycomics Profiling from Histological Tissues. <em>Anal. Chem.<\/em> <strong>2014<\/strong>, <em>86<\/em>, 9670-9678.<a href=\"http:\/\/dx.doi.org\/10.1021\/ac5022216\" target=\"blank\"><span style=\"color: #3300ff;\" color=\"#3300ff\"> Pubmed Link<\/span><\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Cell surface and extracellular matrix glycoproteins and proteoglycans modulate the accessibility of growth factor ligands to cell surface receptors, thereby influencing signalling transduction.\u00a0 It is therefore not surprising that dysregulation of homeostasis of cell surface and extracellular matrix molecules is a hallmark of neurological diseases and cancers.\u00a0 We developed a method for printing glycosidase or [&hellip;]<\/p>\n","protected":false},"author":1067,"featured_media":0,"parent":6,"menu_order":8,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/pages\/2596"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/users\/1067"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/comments?post=2596"}],"version-history":[{"count":6,"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/pages\/2596\/revisions"}],"predecessor-version":[{"id":2605,"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/pages\/2596\/revisions\/2605"}],"up":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/pages\/6"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/media?parent=2596"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}