{"id":2522,"date":"2016-07-27T15:57:00","date_gmt":"2016-07-27T19:57:00","guid":{"rendered":"https:\/\/www.bumc.bu.edu\/msr\/?page_id=2522"},"modified":"2016-07-27T16:00:06","modified_gmt":"2016-07-27T20:00:06","slug":"top-down-ms-of-post-translationally-modified-proteins","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/msr\/research-topics\/top-down-ms-of-post-translationally-modified-proteins\/","title":{"rendered":"Top-down MS of post-translationally modified proteins"},"content":{"rendered":"<p>The analysis of misfolded proteins related to amyloid diseases is a longstanding research focus at the MSR:<\/p>\n<ul>\n<LI>Li, X.; Yu, X.; Costello, C. E.; Lin, C.; O&#8217;Connor, P. B. Top-down study of beta2-microglobulin deamidation. <em>Anal. Chem.<\/em> <strong>2012<\/strong>, <em>84<\/em>, 6150-7.<A HREF=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22746280\" target = \"blank\"><font color=\"#3300ff\"> Pubmed Link<\/font><\/A><\/LI><br \/>\n<LI>Theberge, R.; Infusini, G.; Tong, W.; McComb, M. E.; Costello, C. E. Top-Down Analysis of Small Plasma Proteins Using an LTQ-Orbitrap. Potential for Mass Spectrometry-Based Clinical Assays for Transthyretin and Hemoglobin. <em>Int J Mass Spectrom<\/em> <strong>2011<\/strong>, <em>300<\/em>, 130-142.<A HREF=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/21607198\" target = \"blank\"><font color=\"#3300ff\"> Pubmed Link<\/font><\/A><\/LI><br \/>\n<LI>Theberge, R.; Dikler, S.; Heckendorf, C.; Chui, D. H.; Costello, C. E.; McComb, M. E. MALDI-ISD Mass Spectrometry Analysis of Hemoglobin Variants: a Top-Down Approach to the Characterization of Hemoglobinopathies. <em>J Am Soc Mass Spectrom<\/em> <strong>2015<\/strong>, <em>26<\/em>, 1299-310.<A HREF=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26002792\" target = \"blank\"><font color=\"#3300ff\"> Pubmed Link<\/font><\/A><\/LI>\n<\/ul>\n<p>We demonstrated feasibility of top-down MS of glycoproteins:<\/p>\n<ul>\n<LI>Gault, J.; Malosse, C.; Machata, S.; Millien, C.; Podglajen, I.; Ploy, M. C.; Costello, C. E.; Dumenil, G.; Chamot-Rooke, J. Complete posttranslational modification mapping of pathogenic Neisseria meningitidis pilins requires top-down mass spectrometry. <em>Proteomics<\/em> <strong>2014<\/strong>, <em>14<\/em>, 1141-51.<A HREF=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24459079\" target = \"blank\"><font color=\"#3300ff\"> Pubmed Link<\/font><\/A><\/LI><br \/>\n<LI>Bourgoin-Voillard, S.; Leymarie, N.; Costello, C. E. Top-down tandem mass spectrometry on RNase A and B using a Qh\/FT-ICR hybrid mass spectrometer. <em>Proteomics<\/em> <strong>2014<\/strong>, <em>14<\/em>, 1174-84.<A HREF=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24687996\" target = \"blank\"><font color=\"#3300ff\"> Pubmed Link<\/font><\/A><\/LI>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>The analysis of misfolded proteins related to amyloid diseases is a longstanding research focus at the MSR: Li, X.; Yu, X.; Costello, C. E.; Lin, C.; O&#8217;Connor, P. B. Top-down study of beta2-microglobulin deamidation. Anal. Chem. 2012, 84, 6150-7. Pubmed Link Theberge, R.; Infusini, G.; Tong, W.; McComb, M. E.; Costello, C. E. Top-Down Analysis [&hellip;]<\/p>\n","protected":false},"author":1067,"featured_media":0,"parent":6,"menu_order":10,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/pages\/2522"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/users\/1067"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/comments?post=2522"}],"version-history":[{"count":2,"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/pages\/2522\/revisions"}],"predecessor-version":[{"id":2526,"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/pages\/2522\/revisions\/2526"}],"up":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/pages\/6"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/media?parent=2522"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}