{"id":2512,"date":"2016-07-27T15:35:38","date_gmt":"2016-07-27T19:35:38","guid":{"rendered":"https:\/\/www.bumc.bu.edu\/msr\/?page_id=2512"},"modified":"2018-10-10T14:17:19","modified_gmt":"2018-10-10T18:17:19","slug":"glycoproteomics","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/msr\/research-topics\/glycoproteomics\/","title":{"rendered":"Glycoproteomics"},"content":{"rendered":"<p>The MSR is developing methods for assignment of site-specific glycosylation using MS and tandem MS. These efforts include development of new tandem MS methods, bioinformatics, and applications to biomedical projects.<\/p>\n<p>Here are our reviews on glycoproteomics:<img loading=\"lazy\" src=\"\/msr\/files\/2016\/07\/ancham_v084i007-226x300.jpg\" alt=\"ancham_v084i007.indd\" class=\" size-medium wp-image-2565 alignright\" height=\"300\" width=\"226\" srcset=\"https:\/\/www.bumc.bu.edu\/msr\/files\/2016\/07\/ancham_v084i007-226x300.jpg 226w, https:\/\/www.bumc.bu.edu\/msr\/files\/2016\/07\/ancham_v084i007-771x1024.jpg 771w\" sizes=\"(max-width: 226px) 100vw, 226px\" \/><\/p>\n<ul>\n<li>Leymarie, N.; Zaia, J. Effective use of mass spectrometry for glycan and glycopeptide structural analysis. <em>Anal. Chem.<\/em> <strong>2012<\/strong>, <em>84<\/em>, 3040-8.<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22360375\" target=\"blank\"><span style=\"color: #3300ff;\" color=\"#3300ff\"> Pubmed Link<\/span><\/a><\/li>\n<li>Hu, H.; Khatri, K.; Klein, J.; Leymarie, N.; Zaia, J. A review of methods for interpretation of glycopeptide tandem mass spectral data. <em>Glycoconj J<\/em> <strong>2016<\/strong>, <em>33<\/em>, 285-296.<A HREF=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26612686\" target = \"blank\"><font color=\"#3300ff\"> Pubmed Link<\/font><\/A><\/LI>\n<li>Hu, H.; Khatri, K.; Zaia, J. Algorithms and design strategies towards automated glycoproteomics analysis. <em>Mass Spectrom Rev<\/em> <strong>2017<\/strong>, <em>36<\/em>, 475-498.<A HREF=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26728195\" target = \"blank\"><font color=\"#3300ff\"> Pubmed Link<\/font><\/A><\/LI>\n<li>Sethi, M. K.; Zaia, J. Extracellular matrix proteomics in schizophrenia and Alzheimer&#8217;s disease. <em>Anal Bioanal Chem<\/em> <strong>2017<\/strong>, <em>409<\/em>, 379-394.<A HREF=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27601046\" target = \"blank\"><font color=\"#3300ff\"> Pubmed Link<\/font><\/A><\/li>\n<\/ul>\n<p>We organized an intra-laboratory study on assignment of site specific glycoprotein glycosylation:<\/p>\n<ul>\n<li>Leymarie, N.; Griffin, P. J.; Jonscher, K.; Kolarich, D.; Orlando, R.; <strong>McComb, M.; Zaia, J (corresponding authors) <\/strong>.; Aguilan, J.; Alley, W. R.; Altmann, F.; Ball, L. E.; Basumallick, L.; Bazemore-Walker, C. R.; Behnken, H.; Blank, M. A.; Brown, K. J.; Bunz, S. C.; Cairo, C. W.; Cipollo, J. F.; Daneshfar, R.; Desaire, H.; Drake, R. R.; Go, E. P.; Goldman, R.; Gruber, C.; Halim, A.; Hathout, Y.; Hensbergen, P. J.; Horn, D. M.; Hurum, D.; Jabs, W.; Larson, G.; Ly, M.; Mann, B. F.; Marx, K.; Mechref, Y.; Meyer, B.; Moginger, U.; Neususs, C.; Nilsson, J.; Novotny, M. V.; Nyalwidhe, J. O.; Packer, N. H.; Pompach, P.; Reiz, B.; Resemann, A.; Rohrer, J. S.; Ruthenbeck, A.; Sanda, M.; Schulz, J. M.; Schweiger-Hufnagel, U.; Sihlbom, C.; Song, E.; Staples, G. O.; Suckau, D.; Tang, H.; Thaysen-Andersen, M.; Viner, R. I.; An, Y.; Valmu, L.; Wada, Y.; Watson, M.; Windwarder, M.; Whittal, R.; Wuhrer, M.; Zhu, Y.; Zou, C. Interlaboratory Study on Differential Analysis of Protein Glycosylation by Mass Spectrometry: the ABRF Glycoprotein Research Multi-Institutional Study 2012. <em>Molecular &amp; cellular proteomics : MCP<\/em> <strong>2013<\/strong>, <em>12<\/em>, 2935-2951.<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/23764502\" target=\"blank\"><span style=\"color: #3300ff;\" color=\"#3300ff\"> Pubmed Link<\/span><\/a><\/li>\n<\/ul>\n<p>We developed methods for assigning site-specific proteoglycan glycosylation<\/p>\n<ul>\n<LI>Klein, J. A.; Meng, L.; Zaia, J. Deep sequencing of complex proteoglycans: a novel strategy for high coverage and site-specific identification of glycosaminoglycan-linked peptides. <em>Mol Cell Proteomics<\/em> <strong>2018<\/strong>, <em>17<\/em>, 1578-1590.<A HREF=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29773674\" target = \"blank\"><font color=\"#3300ff\"> Pubmed Link<\/font><\/A><\/LI>\n<\/ul>\n<p>We developed methods for assigning site specific glycosylation in viral proteins:<\/p>\n<ul>\n<li>Crouch, E.; Nikolaidis, N.; McCormack, F.; McDonald, B.; Allen, K<img loading=\"lazy\" src=\"\/msr\/files\/2016\/07\/MCP-232x300.gif\" alt=\"MCP\" class=\" size-medium wp-image-2560 alignright\" height=\"300\" width=\"232\" \/>.; Rynkiewicz, M.; Cafarella, T.; White, M.; Lewnard, K.; Leymarie, N.; Zaia, J.; Seaton, B.; Hartshorn, K. Mutagenesis of SP-D informed by evolution and xray crystallography enhances defenses against Influenza A Virus in vivo. <em>J Biol Chem<\/em> <strong>2011<\/strong>, <em>286<\/em>, 40681-92.<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/21965658\" target=\"blank\"><span style=\"color: #3300ff;\" color=\"#3300ff\"> Pubmed Link<\/span><\/a><\/li>\n<li>Jiang, J.; Tian, F.; Cai, Y.; Qian, X.; Costello, C. E.; Ying, W. Site-specific qualitative and quantitative analysis of the N- and O-glycoforms in recombinant human erythropoietin. <em>Anal Bioanal Chem<\/em> <strong>2014<\/strong>, <em>406<\/em>, 6265-74.<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25080026\" target=\"blank\"><span style=\"color: #3300ff;\" color=\"#3300ff\"> Pubmed Link<\/span><\/a><\/li>\n<li>Khatri, K.; Staples, G. O.; Leymarie, N.; Leon, D. R.; Turi\u00e1k, L.; Huang, Y.; Yip, S.; Hu, H.; Heckendorf, C. F.; Zaia, J. Confident Assignment of Site-Specific Glycosylation in Complex Glycoproteins in a Single Step. <em>J. Proteome Res.<\/em> <strong>2014<\/strong>, <em>13<\/em>, 4347-4355.<a href=\"http:\/\/dx.doi.org\/10.1021\/pr500506z\" target=\"blank\"><span style=\"color: #3300ff;\" color=\"#3300ff\"> Pubmed Link<\/span><\/a><\/li>\n<li>Khatri, K.; Klein, J. A.; White, M. R.; Grant, O. C.; Leymarie, N.; Woods, R. J.; Hartshorn, K. L.; Zaia, J. Integrated Omics and Computational Glycobiology Reveal Structural Basis for Influenza A Virus Glycan Microheterogeneity and Host Interactions. <em>Mol Cell Proteomics<\/em> <strong>2016<\/strong>, <em>15<\/em>, 1895-912.<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26984886\" target=\"blank\"><span style=\"color: #3300ff;\" color=\"#3300ff\"> Pubmed Link<\/span><\/a><\/li>\n<li>van Eijk, M.; Rynkiewicz, M. J.; Khatri, K.; Leymarie, N.; Zaia, J.; White, M. R.; Hartshorn, K. L.; Cafarella, T. R.; van Die, I.; Hessing, M.; Seaton, B. A.; Haagsman, H. P. Lectin-mediated binding and sialoglycans of porcine surfactant protein D synergistically neutralize influenza A virus. <em>J Biol Chem<\/em> <strong>2018<\/strong>, <em>293<\/em>, 10646-10662.<A HREF=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29769321\" target = \"blank\"><font color=\"#3300ff\"> Pubmed Link<\/font><\/A><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>The MSR is developing methods for assignment of site-specific glycosylation using MS and tandem MS. These efforts include development of new tandem MS methods, bioinformatics, and applications to biomedical projects. Here are our reviews on glycoproteomics: Leymarie, N.; Zaia, J. Effective use of mass spectrometry for glycan and glycopeptide structural analysis. Anal. Chem. 2012, 84, [&hellip;]<\/p>\n","protected":false},"author":1067,"featured_media":0,"parent":6,"menu_order":6,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/pages\/2512"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/users\/1067"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/comments?post=2512"}],"version-history":[{"count":21,"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/pages\/2512\/revisions"}],"predecessor-version":[{"id":3362,"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/pages\/2512\/revisions\/3362"}],"up":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/pages\/6"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/msr\/wp-json\/wp\/v2\/media?parent=2512"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}