{"id":43772,"date":"2017-02-08T15:15:54","date_gmt":"2017-02-08T20:15:54","guid":{"rendered":"http:\/\/www.bumc.bu.edu\/busm\/?p=43772"},"modified":"2017-02-10T15:26:27","modified_gmt":"2017-02-10T20:26:27","slug":"procedure-to-measure-cell-energy-production","status":"publish","type":"post","link":"https:\/\/www.bumc.bu.edu\/camed\/2017\/02\/08\/procedure-to-measure-cell-energy-production\/","title":{"rendered":"Researchers Develop Software to Measure Cell Energy Production"},"content":{"rendered":"

Collaborative work between researchers from BUSM and MIT has resulted in development of a new software tool that enhances measurement and analysis of energy production generated by human immune cells.<\/p>\n

These findings appear in the journal PLOS ONE<\/em>.<\/p>\n

\u201cMeasuring energy production is critical for determining how immune cells fuel inflammation in many diseases such as obesity and diabetes,\u201d explained corresponding author Barbara Nikolajczyk, PhD, associate professor of microbiology at BUSM.<\/p>\n

\"COM-Tcell\"
A healthy T cell<\/figcaption><\/figure>\n

Using this software, BUSM researchers examined peripheral blood cells, including T cells and B cells, from healthy people and from those with type 1 or type 2 diabetes. The cells were analyzed for production of oxygen or lactic acid, two ways of measuring how immune cells produce energy.<\/p>\n

Energy measurement used Extracellular Flux (XF) technology and the newly developed analytical tool, SHORE, to detect shifts in fuel utilization.\u00a0 This procedure can accurately compare peripheral blood cells from healthy people to those with diabetes. Development of this automated tool increases the researchers\u2019 capacity to analyze the large number of cell samples required for rigor in human materials research.<\/p>\n

According to the researchers, this tool will allow them to determine whether dietary intake and drugs would help normalize the chronic inflammation that is known to drive disease development.<\/p>\n

\u201cThis method is a critical advance in the analysis of human immune cells and is available as a free download to serve the scientific community unencumbered,\u201d added Nikolajczyk.<\/p>\n","protected":false},"excerpt":{"rendered":"

BUSM and MIT researchers have developed a new, free software tool that enhances measurement and analysis of immune cell energy production.<\/p>\n","protected":false},"author":12772,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[91,90],"tags":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/posts\/43772"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/users\/12772"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/comments?post=43772"}],"version-history":[{"count":6,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/posts\/43772\/revisions"}],"predecessor-version":[{"id":43969,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/posts\/43772\/revisions\/43969"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/media?parent=43772"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/categories?post=43772"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/tags?post=43772"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}