{"id":99945,"date":"2021-12-17T14:10:33","date_gmt":"2021-12-17T19:10:33","guid":{"rendered":"http:\/\/www.bumc.bu.edu\/busm\/?page_id=99945"},"modified":"2026-03-19T11:49:11","modified_gmt":"2026-03-19T15:49:11","slug":"phenoimager-ht-quantitative-pathology-imaging-system","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/camed\/research-impact\/cores\/phenoimager-ht-quantitative-pathology-imaging-system\/","title":{"rendered":"PhenoImager HT Quantitative Pathology Imaging System"},"content":{"rendered":"

The PhenoImager HT is an upgraded version of the Vectra Polaris image analysis suite, capable of unmixing whole slide images (WSI) during the scanning process. This enables users to perform quantitative analysis such as positive pixel analysis, cell phenotyping, and spatial analysis.<\/strong><\/p>\n

\"Book<\/a>
Akoya Biosciences PhenoImager HT Whole Slide Imaging System<\/figcaption><\/figure>\n

 <\/p>\n

Overall Benefit<\/strong><\/h3>\n

The addition of the PhenoImager HT Quantitative Pathology Imaging System to the BU medical campus will enhance research capabilities by:<\/p>\n

    \n
  1. High-Throughput Imaging<\/strong>: This system allows for rapid whole slide image acquisition of biological specimens, capturing both brightfield and high-plex fluorescent images (up to six colors plus DAPI). This capability enables more efficient utilization of existing research samples.<\/li>\n
  2. Improved Analytical Sensitivity<\/strong>: By employing multispectral unmixing, the system increases signal-to-noise ratios and effectively removes autofluorescence signals, leading to clearer and more reliable imaging results.<\/li>\n
  3. Quantitative Analysis<\/strong>: The integrated image acquisition and advanced analysis pipeline provide precise and objective characterization of morphomolecular signatures, placing them in spatial context for a deeper understanding of biological processes.<\/li>\n
  4. Comprehensive Pathomics Datasets<\/strong>: The system generates continuous pathomics datasets that can be correlated with other biological data (e.g., clinicopathologic, transcriptomic). This integration facilitates more rigorous research approaches, helping to clarify the clinicopathologic correlates of diseases.<\/li>\n<\/ol>\n

    Akoya Biosciences PhenoImager HT Specifications<\/strong><\/h3>\n
    <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    Slide Capacity<\/strong><\/td>\n80+ (continuous loading)<\/td>\n<\/tr>\n
    Output File Format<\/strong><\/td>\n.qptiff and .im3<\/td>\n<\/tr>\n
    Objectives<\/strong><\/td>\n5, 10, 20, 40x<\/td>\n<\/tr>\n
    Image Acquisition<\/strong><\/td>\nBrightfield and 9-channel fluorescence-30 minutes to an hour<\/td>\n<\/tr>\n
    InFORM Image Analysis Features<\/strong><\/td>\nTissue segmentation (machine learning), cell segmentation, phenotyping (machine learning), scoring, spatial analysis<\/td>\n<\/tr>\n
    Scan Method<\/strong><\/td>\nTile<\/td>\n<\/tr>\n
    Z-Stacking<\/strong><\/td>\nNo<\/td>\n<\/tr>\n
    Liquid Crystal Tunable Filter<\/strong><\/td>\nYes<\/td>\n<\/tr>\n
    Continuous Workflow<\/strong><\/td>\nYes<\/td>\n<\/tr>\n
    Multispectral Imaging with Unmixing<\/strong><\/td>\nYes: Now onboard instrument, without need for inform<\/td>\n<\/tr>\n
    Integrated Spectral Unmixing<\/strong><\/td>\nYes<\/td>\n<\/tr>\n
    Autofluorescence Removal<\/strong><\/td>\nYes: Quantitation and isolation using automatically selected AF filter and creation of custom unmixing library using Inform and PhenoChart<\/td>\n<\/tr>\n
    Synthetic Spectra for Unmixing<\/strong><\/td>\nYes<\/td>\n<\/tr>\n
    Multiband Epifluorescence Filters<\/strong><\/td>\nYes<\/td>\n<\/tr>\n
    Flash LEDs for Photobleaching Protection\u00a0<\/strong><\/td>\nYes<\/td>\n<\/tr>\n
    Local Support Team<\/strong><\/td>\nSales Specialist\/Application Scientists (PhD)\/Service Engineer (PhD)\/Bioinformatics Scientist (PhD)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    <\/div><\/div>\n

    PhenoImager HT Epifluorescence Filter Cube Characteristics<\/strong><\/h3>\n
    <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    \u00a0Slot<\/strong><\/span><\/strong><\/span><\/td>\nCube<\/strong><\/span><\/td>\nBand<\/span><\/strong><\/td>\nExcitation (nm)<\/b><\/span><\/td>\nEmission (nm)<\/span><\/strong><\/td>\n<\/tr>\n
    <\/td>\n<\/td>\n<\/td>\nCut on<\/span><\/strong><\/span><\/strong><\/td>\nCut off<\/span><\/strong><\/td>\nCut on<\/span><\/strong><\/td>\nCut off<\/span><\/strong><\/td>\n<\/tr>\n<\/tbody>\n
    1<\/td>\nDAPI\/NIR<\/td>\nDAPI<\/td>\n383<\/td>\n391<\/td>\n411<\/td>\n697<\/td>\n<\/tr>\n
    <\/td>\nNIR-near infrared<\/td>\n719<\/td>\n751<\/td>\n769<\/td>\n847<\/td>\n<\/tr>\n
    2<\/td>\nFITC<\/td>\nFITC<\/td>\n460<\/td>\n500<\/td>\n511<\/td>\n720<\/td>\n<\/tr>\n
    3<\/td>\nCy3<\/td>\nCy3<\/td>\n530<\/td>\n560<\/td>\n574<\/td>\n720<\/td>\n<\/tr>\n
    4<\/td>\nTexas Red<\/td>\nTexas Red<\/td>\n567<\/td>\n589<\/td>\n604<\/td>\n710<\/td>\n<\/tr>\n
    5<\/td>\nViolet\/Cy5<\/td>\nOpal 480<\/td>\n405<\/td>\n443<\/td>\n458<\/td>\n578<\/td>\n<\/tr>\n
    <\/td>\nCy5<\/td>\n593<\/td>\n647<\/td>\n665<\/td>\n734<\/td>\n<\/tr>\n
    6<\/td>\nMBA<\/td>\nDAPI<\/td>\n377<\/td>\n405<\/td>\n440<\/td>\n466<\/td>\n<\/tr>\n
    <\/td>\nOpal 570<\/td>\n528<\/td>\n549<\/td>\n563<\/td>\n579<\/td>\n<\/tr>\n
    <\/td>\nOpal 690<\/td>\n626<\/td>\n672<\/td>\n691<\/td>\n727<\/td>\n<\/tr>\n
    7<\/td>\nMBB<\/td>\nOpal 480<\/td>\n405<\/td>\n443<\/td>\n46<\/td>\n496<\/td>\n<\/tr>\n
    <\/td>\nOpal 620<\/td>\n580<\/td>\n601<\/td>\n615<\/td>\n651<\/td>\n<\/tr>\n
    <\/td>\nOpal 780<\/td>\n717<\/td>\n753<\/td>\n767<\/td>\n898<\/td>\n<\/tr>\n
    8<\/td>\nNBC<\/td>\nOpal 520<\/td>\n474<\/td>\n499<\/td>\n523<\/td>\n541<\/td>\n<\/tr>\n
    9<\/td>\nSample AF<\/td>\nSample AF<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    <\/div><\/div>\n

    Recommended Fluorescent Dyes<\/strong><\/h3>\n
    <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    Akoya Opal Dyes<\/strong><\/span><\/td>\nAlexa\/ATTO Dyes<\/strong><\/span><\/td>\nBiotium CF\u00ae Dyes<\/strong><\/span><\/td>\nCyanine Dyes<\/span><\/strong><\/span><\/strong><\/td>\nOther<\/span><\/strong><\/td>\n<\/tr>\n<\/tbody>\n
    Spectral DAPI<\/strong><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
    Opal 480<\/strong><\/td>\nAF405<\/strong><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
    Opal 520<\/strong><\/td>\nAF488<\/strong><\/td>\nCF488A<\/strong><\/td>\nCy2<\/strong><\/td>\nFITC<\/strong><\/td>\n<\/tr>\n
    Opal 540<\/td>\nAF514<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
    Opal 570<\/strong><\/td>\nAF555<\/strong><\/td>\nCF543<\/strong><\/td>\nCy3<\/strong><\/td>\nTRITC<\/strong><\/td>\n<\/tr>\n
    Opal 620<\/strong><\/td>\nAF594<\/strong><\/td>\nCF594<\/strong><\/td>\nCy3,5<\/strong><\/td>\nTexas Red<\/strong><\/td>\n<\/tr>\n
    Opal 650<\/td>\nAF635<\/td>\nCF640R<\/td>\nCy5<\/td>\n<\/td>\n<\/tr>\n
    Opal 690<\/strong><\/td>\nAF660\/680<\/strong><\/td>\nCF680R<\/strong><\/td>\nCy5,5<\/strong><\/td>\n<\/td>\n<\/tr>\n
    Opal 780<\/strong><\/td>\nAF750<\/strong><\/td>\n<\/td>\nCy7<\/strong><\/td>\n<\/td>\n<\/tr>\n
    Highlighted dyes are part of the motif rapid 7-color acquisition protocol and recommended for maximum efficiency and quality of whole-slide imaging.<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    <\/div><\/div>\n

    Services provided by staff include:<\/strong><\/h3>\n