{"id":93520,"date":"2021-06-08T11:08:44","date_gmt":"2021-06-08T15:08:44","guid":{"rendered":"http:\/\/www.bumc.bu.edu\/busm\/?page_id=93520"},"modified":"2026-05-20T10:05:05","modified_gmt":"2026-05-20T14:05:05","slug":"transmission-electron-microscope-tem","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/camed\/research-impact\/cores\/transmission-electron-microscope-tem\/","title":{"rendered":"Transmission Electron Microscope (TEM)"},"content":{"rendered":"

As part of the Integrated Biomedical Imaging Services (IBIS), the Transmission Electron Microscope (TEM) system at the Boston University Medical Campus provides services for transmission electron microscopy imaging, as well as services for sample preparation and assistance with projects, experiment planning and analysis.<\/p>\n

How to Schedule<\/a> \u2502iLab Scheduler<\/a>\u2502Contact<\/a><\/h3>\n

Instruments, Services and Rates<\/strong><\/h2>\n

JEOL JEM-1400Flash TEM<\/strong><\/h3>\n

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Specifications of JEOL JEM-1400Flash TEM\u00a0<\/strong><\/h4>\n
<\/p>\n\n\n\n\n\n\n\n\n\n\n
Electron Source<\/strong><\/td>\nLaB6 filament<\/td>\n<\/tr>\n
Accelerating Voltage<\/strong><\/td>\n60 kv-120kv<\/td>\n<\/tr>\n
Operation Modes<\/strong><\/td>\nBright Field
\nDark Field
\nDiffraction<\/td>\n<\/tr>\n
Magnification<\/strong><\/td>\nLow Mag Mode 10X to 1,000X
\nStandard Mag Mode 200X to 1.2MX<\/td>\n<\/tr>\n
TEM Resolution<\/strong><\/td>\n0.2 nm lattice<\/p>\n

0.38 nm point to point<\/td>\n<\/tr>\n

Sample Holders<\/strong><\/td>\nJeol quick-change specimen holder with one specimen capacity<\/p>\n

Fischione 2040 dual axis rotating holder<\/td>\n<\/tr>\n

Tilt Angles<\/strong><\/td>\n\u00b125\u00b0 tilt with quick-change specimen holder<\/p>\n

\u00b170\u00b0 tilt with Fischione holder<\/td>\n<\/tr>\n

Image Recording<\/strong><\/td>\nAMT NanoSprint-43M-B Mid-Mount CMOS camera with a large 7915\u00d75436 pixels format. This camera offers 5.5\u03bcm square pixels at the phosphor and images a 43 x 30 mm physical FOV.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

<\/div><\/div>\n

The JEOL JEM-1400 Flash TEM is equipped with:<\/p>\n

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  1. Dual-Axis Fischione Model 2040 specimen holder<\/strong>, which allows for precise in situ specimen in-plane 360-degree rotation that is necessary for automated montaging and serial imaging. This specimen holder features an optimal tilt angle range in narrow gap (~ 5 mm) pole piece geometries, while maintaining microscope resolution. Thus, this holder can also be used for 3D tomography applications.<\/li>\n
  2. SerialEM and IMOD Software<\/strong>, which enables automated image acquisition, registration, and alignment of single- or dual axis tilt series. SerialEM also employs a versatile montaging system that can be used to acquire a large-scale image montage of the entire grid across the TEM sample. The IMOD software suite is a set of post-acquisition programs for processing the 3D tomography tilt series as well as serial section data into 3D reconstructions.<\/li>\n<\/ol>\n

    Leica UCT Ultracut Ultramicrotome<\/strong><\/h3>\n

    \"\"<\/a>This instrument is designed for extremely precise cutting of resin embedded biological specimens. It achieves consistent high quality ultra-thin sections (40-100 nm) for transmission electron microscopy, or semi-thin sections (1-2 \u00b5m) for light microscopy. It features a continuous feed range from 0 to 2.5 \u00b5m and has adjustable cutting speeds of 0.1 to 50 mm\/sec. The system consists of a control box with power cord, a microtome sectioning unit, and a microscope assembly with AO Stereostar 570 zoom body (0.7X \u2013 4.2X) with attached 10X eyepieces. It has a 6V, 10W halogen illumination system.<\/p>\n

    Services provided by TEM staff include<\/strong>:<\/p>\n