{"id":69162,"date":"2019-03-29T07:38:48","date_gmt":"2019-03-29T11:38:48","guid":{"rendered":"http:\/\/www.bumc.bu.edu\/busm\/?page_id=69162"},"modified":"2024-11-05T14:05:10","modified_gmt":"2024-11-05T19:05:10","slug":"qpcr-pcr-software","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/camed\/research-impact\/analytical-instrumentation-core\/qpcr-pcr-software\/","title":{"rendered":"qPCR\/PCR Machines and Software"},"content":{"rendered":"<div class=\"bu-slideshow-container qpcr-machines-&amp;-software autoplay\" id=\"bu-slideshow-container-69262\" data-slideshow-name=\"qpcr-machines-&-software\" data-slideshow-delay=\"5000\" style=\"width: auto; \"><div class='slideshow-loader active'><div class='loader-animation'><\/div><p>loading slideshow...<\/p><\/div><div class=\"bu-slideshow-slides\"><ul class=\"bu-slideshow transition-slide\" id=\"bu-slideshow-69262\"><li id=\"bu-slideshow-69262_0\" class=\"slide \"><div class=\"bu-slide-container slide-caption-bottom-left\"><a href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\"><img src=\"\/camed\/files\/2019\/03\/Stepone-Plux-96-well-real-time-PCR-300x300.jpg\" alt=\"\" \/><\/a><div class=\"bu-slide-caption caption-bottom-left\"><p class=\"bu-slide-caption-title\"><a href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\">StepOnePlus\u2122 Real-Time PCR System<\/a><\/p><p class=\"bu-slide-caption-text\">PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. <\/p><\/div><\/div><\/li><li id=\"bu-slideshow-69262_1\" class=\"slide \"><div class=\"bu-slide-container slide-caption-bottom-left\"><a href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\"><img src=\"\/camed\/files\/2019\/03\/Life-Technologies-7900-HT-Fast-Real-Time-PCR.jpg\" alt=\"\" \/><\/a><div class=\"bu-slide-caption caption-bottom-left\"><p class=\"bu-slide-caption-title\"><a href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\">Life Technologies 7900 HT Fast Real-Time PCR System<\/a><\/p><p class=\"bu-slide-caption-text\">High-throughput real-time PCR system that detects and quantitates nucleic acid sequences. <\/p><\/div><\/div><\/li><li id=\"bu-slideshow-69262_2\" class=\"slide \"><div class=\"bu-slide-container slide-caption-bottom-left\"><a href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\"><img src=\"\/camed\/files\/2020\/10\/Quantstudio-12K-Flex-qPCR-System.jpg\" alt=\"\" \/><\/a><div class=\"bu-slide-caption caption-bottom-left\"><p class=\"bu-slide-caption-title\"><a href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\">Quantstudio 12K Flex qPCR System<\/a><\/p><p class=\"bu-slide-caption-text\">Modern powerful real-time qPCR instrument with five heating blocks: 96 well, 96fast, 384 well, 384-Array Card, and 3200-Open Array.<\/p><\/div><\/div><\/li><\/ul><\/div><div class=\"bu-slideshow-navigation-container\"><ul class=\"bu-slideshow-navigation nav-icon\" id=\"bu-slideshow-nav-69262\" aria-hidden=\"true\"><li><a href=\"#\" id=\"pager-1\" class=\" active\" aria-hidden=\"true\"><span>1<\/span><\/a><\/li> <li><a href=\"#\" id=\"pager-2\" class=\"\" aria-hidden=\"true\"><span>2<\/span><\/a><\/li> <li><a href=\"#\" id=\"pager-3\" class=\"\" aria-hidden=\"true\"><span>3<\/span><\/a><\/li> <\/ul><\/div><\/div>\n<hr \/>\n<p><a href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\"><img loading=\"lazy\" title=\"Book it now!\" class=\"alignright wp-image-89555 size-full\" alt=\"\" src=\"\/camed\/files\/2020\/10\/Quantstudio-12K-Flex-qPCR-System.jpg\" width=\"353\" height=\"478\" \/><\/a><\/p>\n<h3><strong>Quantstudio 12K Flex qPCR System<\/strong><\/h3>\n<p><strong>All-In-One qPCR Instrument<br \/>\n<\/strong><br \/>\nCombining flexible throughput capabilities with a streamlined workflow, the Applied Biosystems&#x2122; <a style=\"text-decoration: none;\" href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\">QuantStudio 12K (QS12K) Flex<\/a> system takes you from targeted discovery through confirmation and screening, all on a single platform.<a name=\"quantstudio\"><\/a><\/p>\n<p><strong>Increased Throughput<\/strong><\/p>\n<p>The QS12K Flex system is capable of running up to four Applied Biosystems&#x2122; OpenArray&#x2122; plates in a single run. This is the equivalent to 32 traditional 384-well qPCR plates or 12,000 data points.<\/p>\n<p><strong>Five Interchangeable Blocks<br \/>\n<\/strong><br \/>\nThe QS12K Flex instrument is equipped with five different heating block that you can choose from (OpenArray plate, TaqMan Array Card, 384-well, Standard 96-well, and Fast 96-well).<\/p>\n<p><strong>Note: Please specify the plate type when you are reserving the instrument on the <\/strong><a style=\"text-decoration: none;\" href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\"><strong>scheduler<\/strong><\/a><\/p>\n<p>For additional information about assays available, please visit the <a style=\"text-decoration: none;\" href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-assays.html?gclid=CjwKCAjw3-bzBRBhEiwAgnnLCgUDKxjVApj4g5qZ-ArwLqMpMbwCQhgI3pdss1FRCz0Vq7X_eYYFqRoCpawQAvD_BwE&amp;cid=gsd_pcr_sbu_r01_us_cp1297_pjt4711_gsd00000_0se_gaw_bt_pur_10033082&amp;s_kwcid=AL!3652!3!349859337091!e!!g!!taqman&amp;ef_id=CjwKCAjw3-bzBRBhEiwAgnnLCgUDKxjVApj4g5qZ-ArwLqMpMbwCQhgI3pdss1FRCz0Vq7X_eYYFqRoCpawQAvD_BwE:G:s&amp;s_kwcid=AL!3652!3!349859337091!e!!g!!taqman\">TaqMan qPCR<\/a> page.<\/p>\n<p><strong>DOM Location:\u00a0<\/strong>650 Albany Street, Rm X-830<br \/>\n<strong><br \/>\nInternal Rate (Unassisted): $34 per run (up to 2 hours)<br \/>\nInternal Rate (Assisted): $94 per run (up to 2 hours)<br \/>\nExternal Rate (Unassisted): $60.52 per run (up to 2 hours)<br \/>\nExternal Rate (Assisted): $167 per run (up to 2 hours)<\/strong><\/p>\n<p><strong>Instrument Manager: <\/strong>Matthew Au \u2502<a style=\"text-decoration: none;\" href=\"mailto:mau115@bu.edu\">mau115@bu.edu<\/a><\/p>\n<h3><strong>How to Schedule<\/strong><\/h3>\n<p>Please login to <a href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\">iLab scheduling system<\/a> for equipment time or services. For new users please follow the steps outlined in<span> <\/span><a href=\"https:\/\/www.bumc.bu.edu\/camed\/research\/cores\/accessing-cores\/information-for-new-users\/\">Information for New Users<\/a>.<\/p>\n<hr \/>\n<h3><strong>StepOnePlus&#x2122; Real-Time PCR System<\/strong><\/h3>\n<p><a href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\"><img loading=\"lazy\" title=\"Book it now!\" class=\"alignleft wp-image-35722 size-full\" alt=\"StepOne Plus 96 Well Real Time PCR\" src=\"\/camed\/files\/2016\/07\/Stepone-Plux-96-well-real-time-PCR.jpg\" width=\"420\" height=\"387\" \/><\/a><br \/>\n<a name=\"stepone\"><\/a><br \/>\nThe <a style=\"text-decoration: none;\" href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\">StepOnePlus&#x2122; Real-Time PCR System<\/a> is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus&#x2122; Real-Time PCR System is equipped with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus&#x2122; Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.<\/p>\n<p>To download the latest version of the StepOne&#x2122; Plus v2.3 software onto your personal computer just click <a style=\"text-decoration: none;\" href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/technical-resources\/software-downloads\/StepOne-and-StepOnePlus-Real-Time-PCR-System.html\">here.<\/a><\/p>\n<p><strong>DOM Location:\u00a0<\/strong>650 Albany Street, Rm X-830<br \/>\n<strong><br \/>\nInternal Rate (Unassisted): $34 per run (up to 2 hours)<br \/>\nInternal Rate (Assisted): $94 per run (up to 2 hours)<br \/>\nExternal Rate (Unassisted): $60.52 per run (up to 2 hours)<br \/>\nExternal Rate (Assisted): $167 per run (up to 2 hours)<\/strong><\/p>\n<p><strong>Instrument Manager: <\/strong>Matthew Au \u2502<a style=\"text-decoration: none;\" href=\"mailto:mau115@bu.edu\">mau115@bu.edu<\/a><\/p>\n<h3><strong>How to Schedule<\/strong><\/h3>\n<p>Please login to <a href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\">iLab scheduling system<\/a> for equipment time or services. For new users please follow the steps outlined in<span> <\/span><a href=\"https:\/\/www.bumc.bu.edu\/camed\/research\/cores\/accessing-cores\/information-for-new-users\/\">Information for New Users<\/a>.<\/p>\n<hr \/>\n<p><a style=\"text-decoration: none;\" href=\"mailto:mau115@bu.edu\"><\/a><\/p>\n<h3><strong>Primer Express Software v3.0<\/strong><\/h3>\n<p>The\u00a0<strong>Primer Express Software v3.0\u00a0<\/strong>allows you to design your own primers and probes using TaqMan and SYBR Green I dye chemistries for gene quantitation and allelic discrimination (SNP) real-time PCR applications.<br \/>\n<a name=\"7900ht\"><\/a><a name=\"software\"><\/a><br \/>\n<strong>DOM Location: <\/strong>650 Albany Street, Rm X-830<br \/>\n<strong>Instrument Manager:\u00a0<\/strong>Lynn Lingyi Deng, PhD \u2502<a style=\"text-decoration: none;\" href=\"mailto:lynndeng@bu.edu\">lynndeng@bu.edu<\/a><\/p>\n<h3><strong>How to Schedule<\/strong><\/h3>\n<p>Please login to <a href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\">iLab scheduling system<\/a> for equipment time or services. For new users please follow the steps outlined in<span> <\/span><a href=\"https:\/\/www.bumc.bu.edu\/camed\/research\/cores\/accessing-cores\/information-for-new-users\/\">Information for New Users<\/a>.<\/p>\n<hr \/>\n<p><a style=\"text-decoration: none;\" href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\"><img loading=\"lazy\" title=\"Book it now!\" class=\"alignleft wp-image-35720 size-full\" alt=\"Life Technologies 7900 HT Fast Real Time PCR\" src=\"\/camed\/files\/2016\/07\/Life-Technologies-7900-HT-Fast-Real-Time-PCR.jpg\" width=\"378\" height=\"234\" \/><\/a><\/p>\n<h3><strong>Life Technologies 7900 HT Fast Real-Time PCR<\/strong><\/h3>\n<p>The\u00a0<a style=\"text-decoration: none;\" href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\">Life Technologies 7900 HT Fast Real-Time PCR<\/a> system by Applied Biosystems is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. The 7900HT is also an extremely versatile research tool with both 96-well (fast) and 384-well thermal cycling block formats available. When used with fast reagents, the fast 96-well block reduces run times from 2 hours to about 30 minutes.<\/p>\n<p><strong>Note:<\/strong> <strong>Please specify the plate type (96-well fast or 384-well) when reserving the instrument on the <\/strong><a style=\"text-decoration: none;\" href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\"><strong>scheduler<\/strong><\/a><\/p>\n<p><strong>DOM Location:\u00a0650 Albany Street, Room X-830<\/strong><\/p>\n<p><strong>Internal Rate (Unassisted): $34 per run (up to 2 hours)<br \/>\nInternal Rate (Assisted): $94 per run (up to 2 hours)<br \/>\nExternal Rate (Unassisted): $60.52 per run (up to 2 hours)<br \/>\nExternal Rate (Assisted): $167 per run (up to 2 hours)<\/strong><\/p>\n<p><strong><strong>Instrument Manager: <\/strong><\/strong>Matthew Au \u2502<a style=\"text-decoration: none;\" href=\"mailto:mau115@bu.edu\">mau115@bu.edu<\/a><\/p>\n<h3><strong>How to Schedule<\/strong><\/h3>\n<p>Please login to <a href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\">iLab scheduling system<\/a> for equipment time or services. For new users please follow the steps outlined in<span> <\/span><a href=\"https:\/\/www.bumc.bu.edu\/camed\/research\/cores\/accessing-cores\/information-for-new-users\/\">Information for New Users<\/a>.<\/p>\n<p><strong>View BU Medical Campus <\/strong><a style=\"text-decoration: none;\" href=\"https:\/\/www.bumc.bu.edu\/about\/map-directions\/\">MAPS<\/a><\/p>\n<p><a style=\"text-decoration: none;\" href=\"https:\/\/www.bumc.bu.edu\/camed\/our-impact\/cores\/analytical-instrumentation-core\/\">\u25c4 Back to<\/a> <strong> Analytical Instrumentation Core<\/strong> <a style=\"text-decoration: none;\" href=\"https:\/\/www.bumc.bu.edu\/camed\/our-impact\/cores\/analytical-instrumentation-core\/\"> website<\/a>.<\/p>\n<p style=\"text-align: right;\"><a style=\"text-decoration: none;\" href=\"#top\">BACK TO TOP\u2191<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Quantstudio 12K Flex qPCR System All-In-One qPCR Instrument Combining flexible throughput capabilities with a streamlined workflow, the Applied Biosystems&#x2122; QuantStudio 12K (QS12K) Flex system takes you from targeted discovery through confirmation and screening, all on a single platform. Increased Throughput The QS12K Flex system is capable of running up to four Applied Biosystems&#x2122; OpenArray&#x2122; plates [&hellip;]<\/p>\n","protected":false},"author":10364,"featured_media":0,"parent":1312,"menu_order":13,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"bu-publication":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/69162"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/users\/10364"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/comments?post=69162"}],"version-history":[{"count":43,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/69162\/revisions"}],"predecessor-version":[{"id":126992,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/69162\/revisions\/126992"}],"up":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/1312"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/media?parent=69162"}],"wp:term":[{"taxonomy":"bu-publication","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/bu-publication?post=69162"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}