{"id":23540,"date":"2015-09-02T08:27:13","date_gmt":"2015-09-02T12:27:13","guid":{"rendered":"http:\/\/www.bumc.bu.edu\/busm\/?page_id=23540"},"modified":"2024-11-06T15:13:35","modified_gmt":"2024-11-06T20:13:35","slug":"ibis","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/camed\/research-impact\/cores\/ibis\/","title":{"rendered":"Integrated Biomedical Imaging Services (IBIS)"},"content":{"rendered":"

The Integrated Biomedical Imaging Services (IBIS) optical microscopy cores at the Boston University Medical campus offer comprehensive, cutting-edge technology and support for a wide range of digital imaging microscopy applications. Located across several facilities, these resources are listed below according to the strengths of each instrument and their supporting facility teams, to better assist you in finding and evaluating the proper equipment and support for your research. These instruments fall into the following categories, best suited for different applications.<\/p>\n

JEOL JEM 1400 Flash TEM<\/a><\/strong><\/h3>\n

\"JEOL<\/a>
\nJEOL JEM 1400 Flash TEM system has (a) Dual-Axis Fischione Model 2040 specimen holder<\/em>, which allows for precise in situ specimen in-plane 360 degree rotation that is necessary for automated montaging and serial imaging. This specimen holder features an optimal tilt angle range in narrow gap (~ 5 mm) pole piece geometries, while maintaining microscope resolution. Thus, this holder can also be used for 3D tomography applications; (b) SerialEM and IMOD Software<\/em>, which enables automated image acquisition, registration, and alignment of single- or dual axis tilt series. SerialEM also employs a versatile montaging system that can be used to acquire a large-scale image montage of the entire grid across the TEM sample. The IMOD software suite is a set of post-acquisition programs for processing the 3D tomography tilt series as well as serial section data into 3D reconstructions.<\/p>\n

<\/h3>\n

Akoya Biosciences PhenoImager HT Whole Slide Imaging System<\/strong><\/a><\/h3>\n

\"\"<\/a>
\nThe PhenoImager HT is an upgraded version of the Vectra Polaris image analysis suite, capable of unmixing whole slide images (WSI) during the scanning process. This enables users to perform quantitative analysis such as positive pixel analysis, cell phenotyping, and spatial analysis.<\/p>\n

 <\/p>\n

\"ZEISS<\/a>
ZEISS LSM 880 with Airyscan<\/figcaption><\/figure>\n

Zeiss LSM 880 Microscope<\/a><\/strong><\/h3>\n

Zeiss LSM 880 with Airyscan enables fast and high-resolution.<\/p>\n

 <\/p>\n

\"carl<\/a>
Zeiss LSM 710 NLO<\/figcaption><\/figure>\n

Zeiss LSM 710 NLO Microscope<\/a><\/strong><\/h3>\n

Optical sectioning of fluorescent tissue using confocal or multiphoton imaging. Time-lapse imaging of living tissue or cell culture. Tile scanning and stitching of composite images. 3D reconstruction of biological tissue\/cells. In vivo time-lapse imaging. FRAP, FRET, Bleaching.<\/p>\n

 <\/p>\n

\"700<\/a>
Zeiss LSM 700 Laser Scanning Confocal<\/figcaption><\/figure>\n

Zeiss LSM 700 Laser Scanning Confocal Microscope<\/a><\/strong><\/h3>\n

Optical sectioning of fixed and stained cells and tissue. Tile scanning. Time-lapse imaging using wide-field or confocal scanning. FRAP.<\/p>\n

 <\/p>\n

\"Leica<\/a>
Leica UCT Ultracut Ultramicrotome<\/figcaption><\/figure>\n

Leica UCT Ultracut Ultramicrotome<\/a><\/strong><\/h3>\n

This instrument is designed for extremely precise cutting of resin embedded biological specimens. It achieves consistent high quality ultra-thin sections (40-100 nm) for transmission electron microscopy, or semi-thin sections (1-2 \u00b5m) for light microscopy. It features a continuous feed range from 0 to 2.5 \u00b5m and has adjustable cutting speeds of 0.1 to 50 mm\/sec. The system consist of a control box with power cord, a microtome sectioning unit, and a microscope assembly with AO Stereostar 570 zoom body (0.7X \u2013 4.2X) with attached 10X eyepieces. It has a 6V, 10W halogen illumination system.<\/p>\n

How to Schedule<\/strong><\/h3>\n

Please login to iLab Scheduling System <\/a> to schedule equipment time or services. For new users please follow the steps outlined in Information for New Users<\/a>.<\/p>\n

View BU<\/strong>MAPS<\/a><\/p>\n

\u25c4 Back to <\/a>Cores, Facilities and Services <\/strong>website<\/a>.<\/p>\n

BACK TO TOP\u2191<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

The Integrated Biomedical Imaging Services (IBIS) optical microscopy cores at the Boston University Medical campus offer comprehensive, cutting-edge technology and support for a wide range of digital imaging microscopy applications. Located across several facilities, these resources are listed below according to the strengths of each instrument and their supporting facility teams, to better assist you […]<\/p>\n","protected":false},"author":10364,"featured_media":0,"parent":1215,"menu_order":24,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"bu-publication":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/23540"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/users\/10364"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/comments?post=23540"}],"version-history":[{"count":50,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/23540\/revisions"}],"predecessor-version":[{"id":127172,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/23540\/revisions\/127172"}],"up":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/1215"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/media?parent=23540"}],"wp:term":[{"taxonomy":"bu-publication","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/bu-publication?post=23540"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}