{"id":22342,"date":"2015-07-24T10:35:55","date_gmt":"2015-07-24T14:35:55","guid":{"rendered":"http:\/\/www.bumc.bu.edu\/busm\/?page_id=22342"},"modified":"2025-08-15T11:33:40","modified_gmt":"2025-08-15T15:33:40","slug":"carl-zeiss-lsm-710-nlo-microscope-system","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/camed\/research-impact\/cores\/ibis\/carl-zeiss-lsm-710-nlo-microscope-system\/","title":{"rendered":"Zeiss LSM 710 NLO Multiphoton Microscope"},"content":{"rendered":"<figure id=\"attachment_22349\" aria-describedby=\"caption-attachment-22349\" style=\"width: 160px\" class=\"wp-caption alignright\"><a href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\"><img loading=\"lazy\" title=\"Book it now!\" class=\"wp-image-22349 size-full\" alt=\"zeiss lsm 710 mlo\" src=\"\/camed\/files\/2015\/07\/zeiss-lsm-710-mlo.jpg\" width=\"150\" height=\"150\" srcset=\"https:\/\/www.bumc.bu.edu\/camed\/files\/2015\/07\/zeiss-lsm-710-mlo.jpg 150w, https:\/\/www.bumc.bu.edu\/camed\/files\/2015\/07\/zeiss-lsm-710-mlo-100x100.jpg 100w\" sizes=\"(max-width: 150px) 100vw, 150px\" \/><\/a><figcaption id=\"caption-attachment-22349\" class=\"wp-caption-text\">Zeiss LSM 710 NLO<\/figcaption><\/figure>\n<p>The <strong>Zeiss LSM 710 NLO Multiphoton Microscope<\/strong> system equipped with 6 visible laser lines (458nm, 488nm, 514nm, 561nm, 594nm, 633nm), a 405nm solid state laser as well as a tunable femtosecond IR pulse laser for multiphoton excitation (Coherent Chameleon Vision\u2013S).<\/p>\n<p>In addition to two descanned internal detectors and a spectral detector which enables lambda scanning and linear unmixing of fluorescent probes, the system has two highly sensitive gallium arsenide non-descanned detectors for collection of emitted light from two-photon experiments. The laser scanning system is coupled to an upright microscope with a motorized stage and is complete with a variety of objective lenses (10X, 20X, 40X, 63X).<\/p>\n<h4><strong>Applications of the system include:<\/strong><\/h4>\n<ul>\n<li>Optical sectioning of fluorescent tissue using confocal or multiphoton imaging<\/li>\n<li>Time-lapse imaging of living tissue or cell culture<\/li>\n<li>Tile scanning and stitching of composite images<\/li>\n<li>3D reconstruction of biological tissue\/cells<\/li>\n<li>In vivo time-lapse imaging<\/li>\n<li>FRAP, FRET, Bleaching<\/li>\n<\/ul>\n<div class=\"tableScroll\"><div><\/p>\n<table width=\"745\" height=\"142\" border=\"0\">\n<tbody>\n<tr style=\"background-color: #575759;\" valign=\"middle\" align=\"left\">\n<td width=\"214\"><span style=\"color: #ffffff;\"><strong>BU Users Rates<\/strong><\/span><\/td>\n<td width=\"214\"><span style=\"color: #ffffff;\"><strong>External Rates<\/strong><\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 214px; background-color: #f1f7f0;\" width=\"214\">$38\/hour unassisted<br \/>\n$75\/hour assisted<br \/>\n$75\/hour training<\/td>\n<td style=\"width: 214px; background-color: #f1f7f0;\" width=\"214\">$150\/hour unassisted<br \/>\n$200\/hour assisted<br \/>\n$200\/hour training<br \/>\nExtended Hours $15 per hour<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><\/div><\/div>\n<h3><strong>Hours<\/strong><\/h3>\n<p>Monday to Friday: 6 a.m. to 7 p.m.<\/p>\n<p><em>Additional hours are available for persons who have been approved by Dr. Trinkaus-Randall.<\/em><\/p>\n<p>Time-lapse imaging experiments will be charged at the base rate for the first four hours; subsequent hours will be charged at the extended time-lapse imaging rate $15 per hour.<\/p>\n<h3><strong>How to Schedule<\/strong><\/h3>\n<p>Please login to<span>\u00a0<\/span><a href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\">iLab<span>\u00a0<\/span><\/a>system to schedule equipment time or services. For new users please follow the steps outlined in <a href=\"https:\/\/www.bumc.bu.edu\/camed\/research\/cores\/accessing-cores\/information-for-new-users\/\">Information for New Users<\/a>.<\/p>\n<h3><strong>Contact<\/strong><\/h3>\n<p><strong>Vickery Trinkaus-Randall, PhD<\/strong><br \/>\nCore Director<br \/>\n(617) 358-1235 |<a href=\"mailto:vickery@bu.edu\">vickery@bu.edu<\/a><\/p>\n<h3><strong>Location\u00a0<\/strong><\/h3>\n<p>BUSM Housman Medical Research Center<br \/>\n72 E. Concord Street, R Building R-815<br \/>\nBoston, MA 02118<\/p>\n<p><strong>View BU<\/strong><a href=\"https:\/\/www.bumc.bu.edu\/about\/map-directions\/\">MAPS<\/a><\/p>\n<p><a href=\"https:\/\/www.bumc.bu.edu\/camed\/research\/cores\/optical-microscopy-cell-and-tissue-imaging-cores\/confocal-microscope-facility\/\"> \u25c4 Back to <\/a><strong>Confocal Microscopes <\/strong> <a href=\"https:\/\/www.bumc.bu.edu\/camed\/research\/cores\/optical-microscopy-cell-and-tissue-imaging-cores\/confocal-microscope-facility\/\">website<\/a><\/p>\n<p style=\"text-align: right;\"><a href=\"#top\">BACK TO TOP\u2191<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Zeiss LSM 710 NLO Multiphoton Microscope system equipped with 6 visible laser lines (458nm, 488nm, 514nm, 561nm, 594nm, 633nm), a 405nm solid state laser as well as a tunable femtosecond IR pulse laser for multiphoton excitation (Coherent Chameleon Vision\u2013S). In addition to two descanned internal detectors and a spectral detector which enables lambda scanning [&hellip;]<\/p>\n","protected":false},"author":10364,"featured_media":0,"parent":23540,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"bu-publication":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/22342"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/users\/10364"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/comments?post=22342"}],"version-history":[{"count":50,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/22342\/revisions"}],"predecessor-version":[{"id":137261,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/22342\/revisions\/137261"}],"up":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/23540"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/media?parent=22342"}],"wp:term":[{"taxonomy":"bu-publication","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/bu-publication?post=22342"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}