{"id":21965,"date":"2015-07-21T10:00:22","date_gmt":"2015-07-21T14:00:22","guid":{"rendered":"http:\/\/www.bumc.bu.edu\/busm\/?page_id=21965"},"modified":"2025-08-15T11:34:32","modified_gmt":"2025-08-15T15:34:32","slug":"zeiss-700-microscope","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/camed\/research-impact\/cores\/ibis\/confocal-microscope-facility\/zeiss-700-microscope\/","title":{"rendered":"Zeiss LSM 700 Microscope"},"content":{"rendered":"
\"ZEISS-LSM-700-Confocal-Lase\"<\/a>
Zeiss LSM 700 Microscope<\/figcaption><\/figure>\n

The Zeiss LSM 700<\/strong> is equipped with four solid state lasers (405, 488, 555, and 639 nm). Available objectives include 10X dry, 20X dry, 40X oil, and 63X oil. Features of the Zeiss ZEN software include single image acquisition, z-stacks, tiling, programming of multiple locations, time-lapse imaging, and fluorescence recovery after photobleaching (FRAP). Using the proprietary Zeiss software (ZEN) or freeware such as ImageJ or FIJI, one can assess intensity values within regions of interests (ROIs), create 3-D projections from z-stacks, generate histograms, and analyze images for co-localization.<\/p>\n

<\/p>\n\n\n\n\n
BUMC Rates<\/strong><\/span><\/td>\nExternal Academic Users Rates<\/strong><\/span><\/td>\nExternal Commercial Users Rates<\/strong><\/span><\/td>\n<\/tr>\n
$22\/hour unassisted
\n$80\/hour training\/assisted<\/td>\n		$50\/hour unassisted
\n$100\/hour training\/assisted<\/td>\n		$100\/hour unassisted
\n$158\/hour training\/assisted
\nExtended Hours $15 per hour<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

<\/div><\/div>\n

Usage<\/b><\/h3>\n

Persons are trained and then evaluated before working independently. Training usually requires about four hours, divided between two sessions. In some cases both parties decide that it is better for the imaging to be performed in an assisted manner.<\/p>\n

Hours<\/strong><\/h3>\n

Monday to Friday: 6 a.m. to 7 p.m.<\/p>\n

Additional hours are available for persons who have been approved by Dr. Trinkaus-Randall.<\/em><\/p>\n

Time-lapse imaging experiments will be charged at the base rate for the first four hours; subsequent hours will be charged at the extended time-lapse imaging rate $15 per hour.<\/p>\n

How to Schedule<\/strong><\/h3>\n

Please login to\u00a0<\/span>iLab\u00a0<\/span><\/a>system to schedule equipment time or services. For new users please follow the steps outlined in\u00a0<\/span>Information for New Users<\/a>.<\/p>\n

Contact<\/strong><\/h3>\n

Vickery Trinkaus-Randall, PhD<\/strong>
\nCore Director
\n(617) 358-1235 |vickery@bu.edu<\/a><\/p>\n

Location\u00a0<\/strong><\/h3>\n

BUSM Housman Medical Research Center
\n72 E. Concord Street, R Building R-815
\nBoston, MA 02118<\/p>\n

View BU<\/strong>MAPS<\/a><\/p>\n

\u25c4 Back to <\/a>Confocal Microscopes <\/strong> website<\/a><\/p>\n

BACK TO TOP\u2191<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

The Zeiss LSM 700 is equipped with four solid state lasers (405, 488, 555, and 639 nm). Available objectives include 10X dry, 20X dry, 40X oil, and 63X oil. Features of the Zeiss ZEN software include single image acquisition, z-stacks, tiling, programming of multiple locations, time-lapse imaging, and fluorescence recovery after photobleaching (FRAP). Using the […]<\/p>\n","protected":false},"author":10364,"featured_media":0,"parent":1393,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"bu-publication":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/21965"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/users\/10364"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/comments?post=21965"}],"version-history":[{"count":45,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/21965\/revisions"}],"predecessor-version":[{"id":137263,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/21965\/revisions\/137263"}],"up":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/1393"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/media?parent=21965"}],"wp:term":[{"taxonomy":"bu-publication","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/bu-publication?post=21965"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}