{"id":21591,"date":"2015-07-16T15:43:32","date_gmt":"2015-07-16T19:43:32","guid":{"rendered":"http:\/\/www.bumc.bu.edu\/busm\/?page_id=21591"},"modified":"2024-12-11T14:51:25","modified_gmt":"2024-12-11T19:51:25","slug":"array-services","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/camed\/research-impact\/analytical-instrumentation-core\/array-services\/","title":{"rendered":"Array Services"},"content":{"rendered":"<h3><strong>The AIC provides real-time RNA and DNA quantification services using qPCR<\/strong><\/h3>\n<p><strong>Pathway PCR arrays<\/strong>\u00a0allow for the analysis of 84 genes (x 4 sample replicates + housekeeping genes) in one 384-well PCR plate to determine the expression profile of a set of genes relevant to a disease or pathway. There are over 100 cataloged arrays in available for human, rat, and mouse lines. If you have a list of targeted genes we can also provide\u00a0<strong>custom arrays<\/strong> for the analysis of 60 to 384 genes of interest specific to your analysis.<\/p>\n<p>Download our\u00a0<a href=\"\/camed\/files\/2024\/12\/RT-PCR-Array-Service-Booklet-2020.pdf\">RT PCR Array Service Booklet <\/a> for more information about our service!<\/p>\n<p>A list of the most popular PCR Arrays can be found below along with general pricing for this service. Please contact <a href=\"mailto:mau115@bu.edu\">Matthew Au<\/a> if you have any further questions or if you would like to schedule an appointment to discuss your research project and goals.<\/p>\n<h3><strong><a href=\"https:\/\/www.qiagen.com\/us\/products\/discovery-and-translational-research\/pcr-qpcr\/qpcr-assays-and-instruments\/mrna-incrna-qpcr-assays-panels\/rt2-profiler-pcr-arrays\/?akamai-feo=off#orderinginformation\" target=\"_blank\" rel=\"noopener noreferrer\">Over 150 Signaling Pathways &amp; Diseases<\/a><\/strong><\/h3>\n<p><a href=\"http:\/\/www.bumc.bu.edu\/analytical-core\/files\/2012\/08\/Array-Outline.png\"><img loading=\"lazy\" title=\"Array Outline\" class=\"alignright wp-image-90\" alt=\"Array Outline\" src=\"http:\/\/www.bumc.bu.edu\/analytical-core\/files\/2012\/08\/Array-Outline-352x636.png\" width=\"304\" height=\"551\" \/><\/a><\/p>\n<p style=\"text-align: center;\"><strong>Most Popular PCR Arrays: <\/strong><strong>(Please specify human, rat, or mouse)<\/strong><strong> <\/strong><\/p>\n<p style=\"text-align: center;\">Inflammatory cytokines and receptors<br \/>\nApoptosis<br \/>\nExtracellular matrix and adhesion molecules<br \/>\nToll-like receptor signaling pathway<br \/>\nAngiogenesis<br \/>\nOxidative stress and antioxidant defense<br \/>\nWnt signaling pathway<br \/>\nSignal transduction pathway finder<br \/>\nCell cycle<br \/>\nFibrosis<br \/>\nEpithelial to mesenchymal transition (EMT)<br \/>\nMitochondria<br \/>\nStress and toxicity pathway finder<br \/>\nLipoprotein signaling and cholesterol metabolism<\/p>\n<h4><strong>Basic Service:\u00a0Purified cDNA supplied to AIC <\/strong><\/h4>\n<ul>\n<li>AIC will plate your cDNA samples and analyze them on one of our three qPCR instruments<\/li>\n<li>Includes the cost of labor, instrument usage and consumables<\/li>\n<li>Does not the cost of MasterMix and\/or assay plates which will be determined separately<\/li>\n<\/ul>\n<p><strong>Internal Rate: $139 per plate<br \/>\nExternal Rate: $235.72 per plate*<\/strong><\/p>\n<p>*Total Costs are also subject to a 15% Administrative Fee<\/p>\n<h4><strong>Full Service:\u00a0RNA extraction, purification, and cDNA synthesis<\/strong><\/h4>\n<ul>\n<li>AIC will extract and purify the RNA samples that you provide<\/li>\n<li>AIC will synthesize the cDNA using the purified RNA<\/li>\n<li>AIC will plate your cDNA samples and analyze them on one of our three qPCR instruments*<\/li>\n<\/ul>\n<p><strong>Internal Rate: $399 per plate (Includes 4 samples)<br \/>\nExternal Rate: $604.92 per plate (Includes 4 samples)**<\/strong><\/p>\n<p>*Does not include the cost of MasterMix and\/or Assay Plates, which will determined separately<\/p>\n<p>*Total Costs are also subject to a 15% Administrative Fee<\/p>\n<h3><strong>Research Services Agreement<\/strong><\/h3>\n<p>The Analytical Instrumentation Core requires that all experiments conducted adhere to IBC measures. Additionally, both parties read and sign the <a href=\"http:\/\/www.bumc.bu.edu\/analytical-core\/files\/2013\/03\/Research-Services-Agreement-Sample.doc\">Research Services Agreement\u00a0<\/a>\u00a0which outlines the terms and conditions upon which the<strong> Core User <\/strong>and <strong>BU-AIC core<\/strong> engage to conduct the study.<\/p>\n<h3><strong>How to Schedule<\/strong><\/h3>\n<p>Please login to<span> <\/span><a href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\">iLab<span> <\/span><\/a>system to schedule equipment time or services. For new users please follow the steps outlined in<span> <\/span><a href=\"https:\/\/www.bumc.bu.edu\/camed\/research\/cores\/accessing-cores\/information-for-new-users\/\">Information for New Users<\/a>.<\/p>\n<h3><strong>Contact<\/strong><\/h3>\n<p>For further information on our Analytical Services or to schedule a meeting to develop a project, please contact:<\/p>\n<p><strong>Matthew Au<\/strong><br \/>\nAssociate Director of the AIC<br \/>\nAIC Lab Manager<br \/>\nBoston University Chobanian &amp; Avedisian School of Medicine<br \/>\n650 Albany Street, Room 830<br \/>\nBoston MA 02118<br \/>\n(617) 358-6363 | <a href=\"mailto:mau115@bu.edu\">mau115@bu.edu<\/a><\/p>\n<p><strong>Lynn Deng<br \/>\n<\/strong>Director of Analytical Instrumentation Core<br \/>\n650 Albany Street, Room 848<br \/>\nBoston MA 02118<br \/>\n(617) 358-6516 | <a href=\"mailto:lynndeng@bu.edu\">lynndeng@bu.edu<\/a><\/p>\n<p><strong>View BU Medical Campus <\/strong><a href=\"https:\/\/www.bumc.bu.edu\/about\/map-directions\/\">MAPS<\/a><\/p>\n<p><a href=\"https:\/\/www.bumc.bu.edu\/camed\/our-impact\/cores\/analytical-instrumentation-core\/\">\u25c4 Back to <\/a><strong>Analytical Instrumentation Core <\/strong><a href=\"https:\/\/www.bumc.bu.edu\/camed\/our-impact\/cores\/analytical-instrumentation-core\/\">website<\/a>.<\/p>\n<p style=\"text-align: right;\"><a href=\"#top\">\u25b2 BACK TO TOP<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The AIC provides real-time RNA and DNA quantification services using qPCR Pathway PCR arrays\u00a0allow for the analysis of 84 genes (x 4 sample replicates + housekeeping genes) in one 384-well PCR plate to determine the expression profile of a set of genes relevant to a disease or pathway. There are over 100 cataloged arrays in [&hellip;]<\/p>\n","protected":false},"author":10364,"featured_media":0,"parent":1312,"menu_order":21,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"bu-publication":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/21591"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/users\/10364"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/comments?post=21591"}],"version-history":[{"count":47,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/21591\/revisions"}],"predecessor-version":[{"id":128468,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/21591\/revisions\/128468"}],"up":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/1312"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/media?parent=21591"}],"wp:term":[{"taxonomy":"bu-publication","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/bu-publication?post=21591"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}