{"id":21579,"date":"2015-07-16T15:32:47","date_gmt":"2015-07-16T19:32:47","guid":{"rendered":"http:\/\/www.bumc.bu.edu\/busm\/?page_id=21579"},"modified":"2024-11-05T13:21:46","modified_gmt":"2024-11-05T18:21:46","slug":"analytical-services","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/camed\/research-impact\/analytical-instrumentation-core\/analytical-services\/","title":{"rendered":"Analytical Services"},"content":{"rendered":"

1) Lipid (Fatty Acids) Analysis with GC\/MS1<\/sup><\/span><\/strong><\/h3>\n

The Analytical Instrumentation Core (AIC) is now offering Fatty Acid analysis where we can detect and quantify both free and total fatty acids through the use of our HP 6890\/5973 Gas Chromatograph and Mass Spectrometer (GC\/MS). Our GC\/MS is designed for analyzing small molecules (up to 1,000 Da) such as fatty acids, lipids, monosaccharides, amino acids and neurotransmitters.<\/p>\n

Currently, we are able to analyze the following sample types*:<\/p>\n

\"Equipment
Equipment for Fatty Acid Extraction<\/figcaption><\/figure>\n
    \n
  1. Plasma<\/li>\n
  2. Blood<\/li>\n
  3. Cell lines<\/li>\n
  4. Tissues<\/li>\n
  5. Cell Filtrates<\/li>\n
  6. Microorganisms (BSL2)<\/li>\n
  7. Other Biological Samples<\/li>\n<\/ol>\n

    *If your sample type is not listed above please let us know and we can discuss other ways of analyzing your sample!<\/p>\n

     <\/p>\n

    Classes of Fatty Acids Monitored:<\/h4>\n

    \"FA\"<\/a><\/h2>\n
      \n
    1. C12:0 Lauric (Dodecanoic) Acid<\/li>\n
    2. C14:0 Myristic (Tetradecanoic) Acid<\/li>\n
    3. C15:0 Pentadecanoic Acid<\/li>\n
    4. C16:0 Palmitic (Hexadecanoic) Acid<\/li>\n
    5. C17:0 Margaric (Heptadecanoic) Acid<\/li>\n
    6. C18:0 Stearic (Octadecanoic) Acid<\/li>\n
    7. C18:1 Oleic Acid<\/li>\n
    8. C18:2 Linoleic Acid<\/li>\n
    9. C18:3 Linolenic Acid<\/li>\n
    10. C20:0 Eicosanoic (Arachidic) Acid<\/li>\n
    11. C20:3 Dihomo- gamma linoleic Acid<\/li>\n
    12. C20:4 Arachidonic Acid<\/li>\n
    13. C22:0 Behenic (Docosanoic) Acid<\/li>\n<\/ol>\n
      \"HP<\/a>
      HP 6890\/5973 CG\/MS<\/figcaption><\/figure>\n

       <\/p>\n

      How We Analyze Your Samples:<\/h4>\n
        \n
      1. FA Standards are first added to samples.<\/li>\n
      2. Lipids are then extracted (saponification is done for total FA only).<\/li>\n
      3. Chemical derivatization occurs (methylation).<\/li>\n
      4. Samples are injected into the GC\/MS for analysis.<\/li>\n
      5. Data is collected and organized into a report.<\/li>\n<\/ol>\n

        How the Results Are Reported<\/b>:<\/b><\/h4>\n

        For Free Fatty Acids Analysis:<\/strong>\"GC<\/a><\/p>\n

          \n
        1. Classes of the free fatty acids in the sample<\/li>\n
        2. Concentration of each free fatty acid (\u00b5g\/ml)<\/li>\n
        3. Percentage (%) of each fatty acid out of the total amount of fatty acids<\/li>\n<\/ol>\n

          For Total Fatty Acids Analysis:<\/strong><\/p>\n

            \n
          1. Classes of the total fatty acids in the sample<\/li>\n
          2. Concentration of each fatty acid\u00a0(\u00b5g\/ml)<\/li>\n
          3. Percentage (%) of each fatty acid out of the total amount of fatty acids<\/li>\n<\/ol>\n
            Pricing: TBD (Project Dependent)<\/h5>\n

            2)\u00a0Sugars or Monosaccharides Analysis with GC\/MS<\/strong><\/span><\/h3>\n

            In addition to fatty acid analysis, the AIC is also offering monosaccharide analysis using our\u00a0HP\u00a06890\/5973 GC\/MS.<\/p>\n

            Currently, we are able to analyze the following sample types:<\/p>\n

              \n
            1. Plasma<\/li>\n
            2. Blood<\/li>\n
            3. Cell lines<\/li>\n
            4. Tissues<\/li>\n
            5. Cell Filtrates<\/li>\n
            6. Microorganisms (BSL2)<\/li>\n
            7. Other Biological Samples<\/li>\n<\/ol>\n

              How We Analyze Your Samples:<\/h4>\n

              First, internal standards will be added to your samples before the actual sample extraction takes place. Next, the sugars are hydrolyzed through acid-catalyzed reactions. The sugars are then reduced, chemically derivatized and then injected into the GC\/MS for analysis. Finally, the data is collected and organized into a report for the user.<\/p>\n

              Classes of Sugars Monitored:<\/h4>\n

              \"Sugars\"<\/a><\/p>\n

                \n
              1. Pentoses<\/li>\n
              2. Hexoses<\/li>\n
              3. Aminosugars<\/li>\n
              4. Glucose<\/li>\n
              5. Galactose<\/li>\n
              6. Mannose<\/li>\n
              7. Fructose<\/li>\n
              8. Rhamnose<\/li>\n
              9. Ribose<\/li>\n
              10. Arabinose<\/li>\n
              11. Xylose<\/li>\n
              12. GlcN<\/li>\n
              13. Others*<\/li>\n<\/ol>\n

                *Please inquire about other sugars that are not on this list to see if we are able to analyze them.<\/p>\n

                \u00a0How the Results Are Reported:<\/h4>\n
                  \n
                1. Classes of the monosaccharides in the sample<\/li>\n
                2. Concentration of each sugar\/monosaccharide (\u00b5g\/ml)<\/li>\n
                3. Percentage ratio of each sugar out of the total sugars (%)<\/li>\n<\/ol>\n
                  Pricing: TBD (Project Dependent)<\/h5>\n

                  3) Other Services for Biochemical and Physiological Analysis<\/span><\/strong><\/h3>\n

                  The Analytical Instrumentation Core possesses multiple analytical instruments to fit the ongoing needs of a wide variety of users. AIC personnel are able to perform various analyses that cater to the needs of individual projects. The analytical instruments include: GC, GC\/MS, HPLC <\/strong>machines, Electrophoresis, Spectrophotometers <\/strong>(including plate readers), a Blood Analyzer, a Chemistry Analyzer, a Seahorse Extracellular Flux Analyzer<\/strong>, qPCR <\/strong>machines (StepOnePlus, ABI 7900HT and QS12K Flex OpeArray) and instruments for the multiplexing<\/strong> of proteins and cytokines. Our services are most beneficial for starting projects, laboratories that are lacking in personnel and expertise, epidemiological studies, and clinical research. As always, all charges will be reasonable and will depend on the specific needs of each project.<\/p>\n

                  Sample Disclosure Form<\/strong><\/h3>\n

                  The Analytical Instrumentation Core requires that all experiments conducted adhere to IBC measures and that a Sample Disclosure Form<\/a> be completed for all samples that are submitted to the core. Samples without the accompanying form will not be accepted under any circumstances.<\/p>\n

                  How to Schedule<\/strong><\/h3>\n

                  Please login to\u00a0<\/span>iLab\u00a0<\/span><\/a>system to schedule equipment time or services. For new users please contact the core director or follow the steps outlined in\u00a0Information for New Users<\/a>.<\/p>\n

                  Contact<\/strong><\/h3>\n

                  For further information on our Analytical Services or to schedule a meeting to develop a project, please contact:<\/p>\n

                  Matthew Au<\/strong>
                  \nAssociate Director of the AIC
                  \nAIC Lab Manager
                  \nAnalytical Instrumentation Core (AIC)
                  \nBoston University Chobanian & Avedisian School of Medicine
                  \n650 Albany Street, Room 830
                  \nBoston MA 02118
                  \n(617) 358-6363 |                   mau115@bu.edu<\/a><\/p>\n

                  Lynn Deng
                  \n<\/strong>Director of Analytical Instrumentation Core
                  \n650 Albany Street, Room 848
                  \nBoston MA 02118
                  \n(617) 358-6516 |                   lynndeng@bu.edu<\/a><\/p>\n

                  View BU Medical Campus <\/strong>MAPS<\/a><\/p>\n

                  \u25c4 Back to<\/a> Analytical Instrumentation Core<\/strong> website<\/a>.<\/p>\n

                  BACK TO TOP\u2191<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

                  1) Lipid (Fatty Acids) Analysis with GC\/MS1 The Analytical Instrumentation Core (AIC) is now offering Fatty Acid analysis where we can detect and quantify both free and total fatty acids through the use of our HP 6890\/5973 Gas Chromatograph and Mass Spectrometer (GC\/MS). Our GC\/MS is designed for analyzing small molecules (up to 1,000 Da) […]<\/p>\n","protected":false},"author":10364,"featured_media":0,"parent":1312,"menu_order":20,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"bu-publication":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/21579"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/users\/10364"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/comments?post=21579"}],"version-history":[{"count":46,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/21579\/revisions"}],"predecessor-version":[{"id":126972,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/21579\/revisions\/126972"}],"up":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/1312"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/media?parent=21579"}],"wp:term":[{"taxonomy":"bu-publication","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/bu-publication?post=21579"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}