{"id":1425,"date":"2015-05-19T14:03:38","date_gmt":"2015-05-19T18:03:38","guid":{"rendered":"https:\/\/www.bumc.bu.edu\/camed\/?page_id=1425"},"modified":"2025-09-11T13:43:13","modified_gmt":"2025-09-11T17:43:13","slug":"mart","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/camed\/research-impact\/cores\/mart\/","title":{"rendered":"Mouse Assisted Reproductive Technology (MART)"},"content":{"rendered":"<p>The <a href=\"https:\/\/www.bu.edu\/researchsupport\/tools-services\/core-facilities\/mart\/\">Mouse Assisted Reproductive Technology (MART)<\/a> Core staff are experienced in collecting mouse sperm, harvesting unfertilized eggs, conducting in-vitro fertilization and embryo transplantation. In addition, to eliminate common mouse pathogens in the offspring born to mice of uncertain health status (example, mice coming from other facilities and countries), we perform cesarean section, embryo transfer, and in-vitro fertilization. In addition to core reproductive technology support, MART is also available for technical training and collaboration on services within the scope of technical expertise.<br \/>\n<div class=\"tableScroll\"><div><\/p>\n<table style=\"background-color: #f6f6f6;\">\n<tbody>\n<tr>\n<td><strong>Mouse Strain Rederivation<\/strong><\/p>\n<ul>\n<li>Import Rederivation: Live Strain Rederivation using caesarean section and cross-fostering<\/li>\n<li>Embryo Transfer (ET ): fresh embryos<\/li>\n<li>Embryo Transfer (ET ): frozen embryos<\/li>\n<li>In Vitro Fertilization (IVF): fresh sperm<\/li>\n<li>In Vitro Fertilization (IVF): frozen sperm<\/li>\n<\/ul>\n<\/td>\n<td><strong>Sperm Cryopreservation<\/strong><\/p>\n<ul>\n<li>Sperm Recovery and Cryopreservation<\/li>\n<\/ul>\n<p><strong>Cryobank Repository Service<\/strong><\/td>\n<\/tr>\n<tr>\n<td><strong>Import and Export of Frozen Sperm<\/strong><\/p>\n<ul>\n<li>MART core may facilitate the import &amp; export of frozen sperm<\/li>\n<\/ul>\n<\/td>\n<td><strong>Genotyping<\/strong><\/p>\n<ul>\n<li>Tail Snips Recovery<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<tr>\n<td><strong>Vasectomy Surgery<\/strong><\/p>\n<ul>\n<li>Creation of pseudopregnant recipient mothers<\/li>\n<\/ul>\n<\/td>\n<td><strong>Generation of Timed-Pregnant Mothers<\/strong><\/p>\n<ul>\n<li>Creation of foster mothers<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<tr style=\"background-color: #918f94;\">\n<td colspan=\"2\"><strong><\/strong><\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\"><strong>Training and Collaborative Services<\/strong><\/p>\n<ul>\n<li>Mouse Aseptic and Survival Surgery<\/li>\n<li>Embryo Culture<\/li>\n<li>Embryo Microsurgery and Microinjection \u2013 Microinjection Workstation<\/li>\n<li>Embryo Microsurgical Transfer \u2013 Infundibulum Embryo Transfer<\/li>\n<li>Embryo Microsurgical Transfer \u2013 Uterine Embryo Transfer<\/li>\n<li>In Vitro Fertilization (IVF)<\/li>\n<li>Creation of Micro-instruments for Embryo Culture, Microinjection, Embryo Transfer<\/li>\n<li>Vasectomy Surgery<\/li>\n<li>Hormone Priming<\/li>\n<li>Estrus Selection<\/li>\n<li>Generation of Genetically Engineered Animal Models using DNA, mRNA, ES Cells using Embryo Microinjection; Gene Editing with CRISPR or TALENs<\/li>\n<li>Embryo Microsurgical Reconstitution: Developmental Fate Assessment<\/li>\n<li>Graduate Student Practicum<\/li>\n<li>Transgenic training modules may be added as required<\/li>\n<li>Mouse model generation (humanization)<\/li>\n<li>Blastocyst complementation (in vivo organ generation)<\/li>\n<li>Organoid generation<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><\/div><\/div><br \/>\nAll of the above procedures are conducted in our ABSL-2 laboratories to preserve the specific pathogen-free health status of our mouse colonies and breeding program.<\/p>\n<p>Disclaimer: \u00a0Overall performance using the above reproductive technologies tends to be a function of genetic strain viability.<\/p>\n<p>Please visit our <a href=\"https:\/\/www.bu.edu\/researchsupport\/tools-services\/core-facilities\/mart\/\">website<\/a> for more information on all of our services, or contact us for scientific help needed to pursue your project. Investigators may request service price through email John Mkandawire at\u00a0<a href=\"mailto:jmmn@bu.edu\">jmmn@bu.edu<\/a>.<\/p>\n<h3><strong>Contact<\/strong><\/h3>\n<p><strong>John Mkandawire<\/strong><br \/>\nCore Services Specialist<br \/>\nCell (781) 866-1201 \u2502 <a href=\"mailto:jmmn@bu.edu\">jmmn@bu.edu<\/a><br \/>\nOffice (617) 358-8429 \u2502 FAX (617) 358-8277<\/p>\n<p><strong>Colleen Thurman <\/strong><br \/>\nDirector, Animal Science Center<br \/>\nOffice (617) 358-5000 |<a href=\"mailto:mailto:cthurman@bu.edu\"> cthurman@bu.edu<\/a><\/p>\n<h3><strong>Location<\/strong><\/h3>\n<p>BU Medical Center<br \/>\n700 Albany St., W707<br \/>\nBoston, MA 02118<\/p>\n<p><strong>View BU Medical Campus <\/strong><a href=\"https:\/\/www.bumc.bu.edu\/about\/map-directions\/\">MAPS<\/a><\/p>\n<p>\u25c4 <a href=\"https:\/\/www.bumc.bu.edu\/camed\/research\/cores\/\"> Back to <\/a><strong>Cores, Facilities and Services <\/strong><a href=\"https:\/\/www.bumc.bu.edu\/camed\/research\/cores\/\">website<\/a>.<\/p>\n<p style=\"text-align: right;\"><a href=\"#top\">BACK TO TOP\u2191<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Mouse Assisted Reproductive Technology (MART) Core staff are experienced in collecting mouse sperm, harvesting unfertilized eggs, conducting in-vitro fertilization and embryo transplantation. In addition, to eliminate common mouse pathogens in the offspring born to mice of uncertain health status (example, mice coming from other facilities and countries), we perform cesarean section, embryo transfer, and [&hellip;]<\/p>\n","protected":false},"author":7398,"featured_media":0,"parent":1215,"menu_order":36,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"bu-publication":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/1425"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/users\/7398"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/comments?post=1425"}],"version-history":[{"count":50,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/1425\/revisions"}],"predecessor-version":[{"id":138022,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/1425\/revisions\/138022"}],"up":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/1215"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/media?parent=1425"}],"wp:term":[{"taxonomy":"bu-publication","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/bu-publication?post=1425"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}