{"id":138703,"date":"2025-10-02T08:11:52","date_gmt":"2025-10-02T12:11:52","guid":{"rendered":"https:\/\/www.bumc.bu.edu\/camed\/?page_id=138703"},"modified":"2025-11-05T08:13:30","modified_gmt":"2025-11-05T13:13:30","slug":"spatial-biology-core","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/camed\/research-impact\/cores\/spatial-biology-core\/","title":{"rendered":"BU Spatial Biology Core"},"content":{"rendered":"<p>The Boston University Spatial Biology Core (SBC) is an in-house core facility providing access to and full-service data acquisition using:<\/p>\n<ul>\n<li><strong>10x Genomics Visium CytAssist<\/strong>\u00a0for spatial transcriptomics assays.<\/li>\n<li><strong>Standard BioTools Imaging Mass Cytometry Systems<\/strong> using metal-tagged antibodies or probes to detect 40+ markers (protein\/RNA) within tissue samples at subcellular resolution.<\/li>\n<\/ul>\n<p>In addition to these technologies, we also offer consultations to help users with preparing spatial assays, executing studies, and processing and analyzing data.<\/p>\n<div class=\"section\">\n<h2>Services and Instrument Use Rates<\/h2>\n<h3>Service Rates<\/h3>\n<div class=\"tableScroll\"><div><\/p>\n<table>\n<thead>\n<tr>\n<th>Service Category<\/th>\n<th>Service<\/th>\n<th>Service Description<\/th>\n<th>Unit<\/th>\n<th>Internal* Rates<\/th>\n<th>External Academic Rates<\/th>\n<th>External+ Non-Academic Rates<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr style=\"background-color: #f6f6f6;\">\n<td>Imaging<\/td>\n<td>Ab Conjugation\/Validation (marker)<\/td>\n<td>Work up for utilization of antibodies in proteomic and transcriptomic workflows<\/td>\n<td><em>Per marker<\/em><\/td>\n<td>$139.00<\/td>\n<td>$227.00<\/td>\n<td>$247.00<\/td>\n<\/tr>\n<tr>\n<td>Imaging<\/td>\n<td>mIF (per sample)<\/td>\n<td>Staining &amp; imaging for transcriptomic workflows or validation for proteomic workflows<\/td>\n<td>Per sample<\/td>\n<td>$61.00<\/td>\n<td>$100,00<\/td>\n<td>$109.00<\/td>\n<\/tr>\n<tr style=\"background-color: #f6f6f6;\">\n<td>Imaging<\/td>\n<td>H&amp;E (per sample)<\/td>\n<td>Staining &amp; imaging for transcriptomic and proteomic workflows<\/td>\n<td>Per sample<\/td>\n<td>$11.00<\/td>\n<td>$18.00<\/td>\n<td>$20.00<\/td>\n<\/tr>\n<tr>\n<td>Proteomics<\/td>\n<td>Ab Panel staining (per sample)<\/td>\n<td>Staining for proteomic workflows<\/td>\n<td>Per sample<\/td>\n<td>$289.00<\/td>\n<td>$473.00<\/td>\n<td>$514.00<\/td>\n<\/tr>\n<tr style=\"background-color: #f6f6f6;\">\n<td>Transcriptomics<\/td>\n<td>RNA QC (per sample)<\/td>\n<td>RNA isolation &amp; QC for transcriptomic workflows<\/td>\n<td>Per sample<\/td>\n<td>$37.00<\/td>\n<td>$60.00<\/td>\n<td>$66.00<\/td>\n<\/tr>\n<tr>\n<td>Training\/Consulting<\/td>\n<td>Consultation\/Analysis Service<\/td>\n<td>Hands-on training, consultation and\/or analysis with a staff member<\/td>\n<td>Per hour<\/td>\n<td>$56.00<\/td>\n<td>$92.00<\/td>\n<td>$100.00<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><\/div><\/div><\/div>\n<h3>Instrument Use Rates<\/h3>\n<div class=\"tableScroll\"><div><\/p>\n<table>\n<thead>\n<tr>\n<th>Service Category<\/th>\n<th>Instruments<\/th>\n<th>Instrument Description<\/th>\n<th>User<\/th>\n<th>Internal* Rates<\/th>\n<th>External Academic Rates<\/th>\n<th>External+ Non-Academic Rates<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>Proteomics<\/td>\n<td>Standard BioTools Image Mass Cytometer<\/td>\n<td>Imaging Mass Cytometry \u2013 40+ Protein \/RNA in situ imaging<\/td>\n<td>Assisted<\/td>\n<td>$166 per hour<\/td>\n<td>$271 per hour<\/td>\n<td>$295 per hour<\/td>\n<\/tr>\n<tr>\n<td><\/td>\n<td><\/td>\n<td><\/td>\n<td>Unassisted<\/td>\n<td>$133 per hour<\/td>\n<td>$216 per hour<\/td>\n<td>$235 per hour<\/td>\n<\/tr>\n<tr>\n<td><\/td>\n<td><\/td>\n<td><\/td>\n<td><\/td>\n<td><\/td>\n<td><\/td>\n<td><\/td>\n<\/tr>\n<tr style=\"background-color: #f6f6f6;\">\n<td>Transcriptomics<\/td>\n<td>10X Visium CytAssist<\/td>\n<td>Visium Whole Transcriptome Spatial Transcriptomics (Standard, HD, 3&#8242; Assays)<\/td>\n<td>Assisted<\/td>\n<td>$589 per run<\/td>\n<td>$963 per run<\/td>\n<td>$1048 per run<\/td>\n<\/tr>\n<tr>\n<td><\/td>\n<td><\/td>\n<td><\/td>\n<td>Unassisted<\/td>\n<td>$195 per run<\/td>\n<td>$319 per run<\/td>\n<td>$347 per run<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><\/div><\/div>\n<p class=\"note\">* Internal Rates: (Principal Investigators within Boston University)<br \/>\n+ External Rates: (Principal Investigators outside Boston University)<\/p>\n<div class=\"section\">\n<h2>Preparing for Using the Spatial Biology Core<\/h2>\n<h3>Workflow<\/h3>\n<p><img loading=\"lazy\" src=\"\/camed\/files\/2025\/10\/Flowchart-for-spatial-biology.jpg\" alt=\"\" width=\"1280\" height=\"720\" class=\"alignnone size-full wp-image-138731\" srcset=\"https:\/\/www.bumc.bu.edu\/camed\/files\/2025\/10\/Flowchart-for-spatial-biology.jpg 1280w, https:\/\/www.bumc.bu.edu\/camed\/files\/2025\/10\/Flowchart-for-spatial-biology-636x358.jpg 636w, https:\/\/www.bumc.bu.edu\/camed\/files\/2025\/10\/Flowchart-for-spatial-biology-1024x576.jpg 1024w, https:\/\/www.bumc.bu.edu\/camed\/files\/2025\/10\/Flowchart-for-spatial-biology-768x432.jpg 768w, https:\/\/www.bumc.bu.edu\/camed\/files\/2025\/10\/Flowchart-for-spatial-biology-540x304.jpg 540w, https:\/\/www.bumc.bu.edu\/camed\/files\/2025\/10\/Flowchart-for-spatial-biology-1200x675.jpg 1200w, https:\/\/www.bumc.bu.edu\/camed\/files\/2025\/10\/Flowchart-for-spatial-biology-992x558.jpg 992w, https:\/\/www.bumc.bu.edu\/camed\/files\/2025\/10\/Flowchart-for-spatial-biology-1080x608.jpg 1080w, https:\/\/www.bumc.bu.edu\/camed\/files\/2025\/10\/Flowchart-for-spatial-biology-854x480.jpg 854w\" sizes=\"(max-width: 1280px) 100vw, 1280px\" \/><\/p>\n<p>Please submit <a href=\"https:\/\/www.bumc.bu.edu\/camed\/research-impact\/cores\/bu-spatial-biology-core\/bu-spatial-biology-request-form\/\">a consultation request<\/a>. Following a consultation session, BU users should log in to the<a href=\"https:\/\/bostonu.ilab.agilent.com\/account\/login\"> iLab system<\/a> <span>(<i>pending- SBC will reach out to schedule<\/i>) to schedule equipment time or services.<\/span><\/p>\n<h3>Hours<\/h3>\n<ul>\n<li>The Core is open 24\/7 for trained users working independently.<\/li>\n<li>Training is available weekdays from 9 a.m. to 5 p.m. by appointment (please request several days in advance).<\/li>\n<li>Each lab can book an instrument for a maximum of 12 hours per day.<\/li>\n<\/ul>\n<h3>Acknowledgments<\/h3>\n<p>We appreciate acknowledgment of the BU Spatial Biology Core in publications that include data acquired using Core equipment or assistance from Core personnel.<\/p>\n<h3>Letters of Support<\/h3>\n<p>We are happy to provide letters of support for grants. Please email us at <a href=\"mailto:spatial@bu.edu\">spatial@bu.edu<\/a>. If given ample time, we can also review imaging sections and provide preliminary data.<\/p>\n<\/div>\n<div class=\"section\">\n<h2>Contact<\/h2>\n<p><strong>Sarah Mazzilli<\/strong><br \/>\nCore Director<br \/>\nPhone: (617) 358-7269<br \/>\nEmail: <a href=\"mailto:mazzilli@bu.edu\">mazzilli@bu.edu<\/a><\/p>\n<h3>Location<\/h3>\n<p>Evans Building (E)<br \/>\n72 E. Concord Street, E641<br \/>\nBoston, MA 02118<\/p>\n<p><strong>View BU Medical Campus <\/strong><a href=\"https:\/\/www.bumc.bu.edu\/about\/map-directions\/\">MAPS<\/a><\/p>\n<p><a href=\"https:\/\/www.bumc.bu.edu\/camed\/our-impact\/cores\/\">\u25c4 Back to<\/a> <strong>Core Facilities and Services<\/strong> <a href=\"https:\/\/www.bumc.bu.edu\/camed\/our-impact\/cores\/\"> website<\/a>.<\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: right;\"><a href=\"#top\">BACK TO TOP\u2191<\/a><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>The Boston University Spatial Biology Core (SBC) is an in-house core facility providing access to and full-service data acquisition using: 10x Genomics Visium CytAssist\u00a0for spatial transcriptomics assays. Standard BioTools Imaging Mass Cytometry Systems using metal-tagged antibodies or probes to detect 40+ markers (protein\/RNA) within tissue samples at subcellular resolution. In addition to these technologies, we [&hellip;]<\/p>\n","protected":false},"author":10364,"featured_media":0,"parent":1215,"menu_order":10,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"bu-publication":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/138703"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/users\/10364"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/comments?post=138703"}],"version-history":[{"count":19,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/138703\/revisions"}],"predecessor-version":[{"id":139579,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/138703\/revisions\/139579"}],"up":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/1215"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/media?parent=138703"}],"wp:term":[{"taxonomy":"bu-publication","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/bu-publication?post=138703"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}