{"id":119721,"date":"2024-02-20T14:44:25","date_gmt":"2024-02-20T19:44:25","guid":{"rendered":"http:\/\/www.bumc.bu.edu\/camed\/?page_id=119721"},"modified":"2025-07-29T07:44:46","modified_gmt":"2025-07-29T11:44:46","slug":"cryo-em-core-facility","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/camed\/research-impact\/cores\/cryo-em-core-facility\/","title":{"rendered":"Cryogenic Electron Microscopy Core"},"content":{"rendered":"<p>The <a href=\"https:\/\/sites.bu.edu\/cryoem\/\">Boston University Cryogenic Electron Microscopy (BU Cryo-EM) Core Facility<\/a> is a custom-designed microscope facility located at the Center for Advanced Biomedical Research on the BU Medical Campus. We are committed to providing exceptional instrumentation, training, and advice for researchers looking to answer their scientific questions using cryo-EM\/cryo-ET techniques. Our goal is to provide technical and scientific support for the entire cryo-EM\/cryo-ET workflow including sample preparation, data collection, and data processing.<\/p>\n<h3><strong>Instruments and Services<\/strong><\/h3>\n<table width=\"100%\">\n<tbody>\n<tr>\n<th style=\"background-color: #303437;\" width=\"4%;\">\n<h6><span style=\"color: #ffffff;\">Description<br \/>\n<\/span><\/h6>\n<\/th>\n<td>\n<div class=\"bu_collapsible_container \" aria-live=\"polite\" data-customize-animation=\"false\"><h4 class=\"bu_collapsible\" aria-expanded=\"false\"tabindex=\"0\" role=\"button\"> Electron Microscope <\/h4><div class=\"bu_collapsible_section\" style=\"display: none;\"> <\/p>\n<h4><strong>Glacios-2 Cryo-TEM<\/strong><\/h4>\n<ul>\n<li>X-FEG electron source<\/li>\n<li>12-slot sample autoloader<\/li>\n<li>Selectris energy filter<\/li>\n<li>Falcon 4i camera<\/li>\n<li>Ceta-D camera (for MicroED)<\/li>\n<\/ul>\n<h4><strong>Rates<\/strong><\/h4>\n<p><strong>BU\/BUMC Users<\/strong>:<\/p>\n<ul>\n<li>$28 per hour &#8211; Weekdays (Monday 9 a.m.- Friday 2 p.m.)<\/li>\n<li>$21 per hour &#8211; Weekend\/Holidays (Friday 2 p.m. &#8211; Monday 9.m.)<\/li>\n<\/ul>\n<p><strong>External Academic Users<\/strong>:<\/p>\n<ul>\n<li>$75 per hour &#8211; Weekdays (Monday 9 a.m.- Friday 2 p.m.)<\/li>\n<li>$70 per hour &#8211; Weekend\/Holidays (Friday 2 p.m. &#8211; Monday 9.m.)<\/li>\n<\/ul>\n<p><strong>External Industry Users<\/strong>:<\/p>\n<ul>\n<li>$264 per hour &#8211; Weekdays (Monday 9 a.m.- Friday 2 p.m.)<\/li>\n<li>$211 per hour &#8211; Weekend\/Holidays (Friday 2 p.m. &#8211; Monday 9.m.)<\/li>\n<\/ul>\n<p style=\"text-align: right;\"><a style=\"text-align: right;\" text-decoration:=\"\" none=\"\" href=\"#top\">BACK TO TOP\u2191<\/a><\/p>\n<p><\/div>\n<\/div>\n<br \/>\n<div class=\"bu_collapsible_container \" aria-live=\"polite\" data-customize-animation=\"false\"><h4 class=\"bu_collapsible\" aria-expanded=\"false\"tabindex=\"0\" role=\"button\"> Other Instruments<\/h4><div class=\"bu_collapsible_section\" style=\"display: none;\"><\/p>\n<ul>\n<li>Vitrobot Mark IV \u2013 (two)<\/li>\n<li>Pelco easiGlow Glow Discharge Unit<\/li>\n<li>Grid Clipping Stations (two)<\/li>\n<li>Autoloader Cassette Loading Station<\/li>\n<\/ul>\n<h4><strong>Rates<\/strong><\/h4>\n<ul>\n<li><strong>BU\/BUMC Users<\/strong>: $19 per hour<\/li>\n<li><strong>External Academic Users<\/strong>: $35 per hour<\/li>\n<li><strong>External Industry Users<\/strong>: $77 per hour<\/li>\n<\/ul>\n<p style=\"text-align: right;\"><a style=\"text-align: right;\" text-decoration:=\"\" none=\"\" href=\"#top\">BACK TO TOP\u2191<\/a><\/p>\n<p><\/div>\n<\/div>\n<br \/>\n<div class=\"bu_collapsible_container \" aria-live=\"polite\" data-customize-animation=\"false\"><h4 class=\"bu_collapsible\" aria-expanded=\"false\"tabindex=\"0\" role=\"button\"> Software <\/h4><div class=\"bu_collapsible_section\" style=\"display: none;\"><\/p>\n<ul>\n<li>EPU with Multigrid functionality<\/li>\n<li>CryoSPARC LIVE<\/li>\n<li>VNC access for remote grid screening and data collection<\/li>\n<li>SmartEPU with Quality Monitor<\/li>\n<li>TEM tomography data acquisition software<\/li>\n<li>SerialEM<\/li>\n<li>Maps 3 for S\/TEM with Correlative Workflow<\/li>\n<li>MicroED software package<\/li>\n<\/ul>\n<p>Software use is included with the microscope and instrument use rates.<\/p>\n<p style=\"text-align: right;\"><a style=\"text-align: right;\" text-decoration:=\"\" none=\"\" href=\"#top\">BACK TO TOP\u2191<\/a><\/p>\n<p><\/div>\n<\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3><strong>How to Schedule<\/strong><\/h3>\n<p>Interested new users, please email the BU Cryo-EM Core Facility at <a href=\"mailto:cryoem@bu.edu\">cryoem@bu.edu<\/a>\u00a0to schedule an initial consultation to discuss how we can best meet your microscope needs and scientific goals.<\/p>\n<figure id=\"attachment_121392\" aria-describedby=\"caption-attachment-121392\" style=\"width: 455px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/sites.bu.edu\/cryoem\/instruments\/\"><img loading=\"lazy\" src=\"\/camed\/files\/2024\/05\/new-cryon-picture-445x636.jpg\" alt=\"Cryogenic Electron Microscopy\" class=\"wp-image-121392 size-medium\" width=\"445\" height=\"636\" srcset=\"https:\/\/www.bumc.bu.edu\/camed\/files\/2024\/05\/new-cryon-picture-445x636.jpg 445w, https:\/\/www.bumc.bu.edu\/camed\/files\/2024\/05\/new-cryon-picture-717x1024.jpg 717w, https:\/\/www.bumc.bu.edu\/camed\/files\/2024\/05\/new-cryon-picture-768x1097.jpg 768w, https:\/\/www.bumc.bu.edu\/camed\/files\/2024\/05\/new-cryon-picture.jpg 809w\" sizes=\"(max-width: 445px) 100vw, 445px\" \/><\/a><figcaption id=\"caption-attachment-121392\" class=\"wp-caption-text\">Cryogenic Electron Microscopy<\/figcaption><\/figure>\n<h3><strong>Contact<\/strong><\/h3>\n<p><strong>Esther Bullitt, PhD<\/strong><br \/>\nProfessor and Core Facility Director (Team Lead)<br \/>\n<a href=\"mailto:bullitt@bu.edu\">bullitt@bu.edu<\/a><\/p>\n<p><strong>Chad Hicks, PhD<\/strong><br \/>\nCryo-EM Scientist (Core Facility Manager)<br \/>\n<a href=\"mailto:hickscw@bu.edu\">hickscw@bu.edu<\/a><\/p>\n<p><strong>Christopher Akey, PhD<\/strong><br \/>\nProfessor (Core Facility Advisor)<br \/>\n<a href=\"mailto:cakey@bu.edu\">cakey@bu.edu<\/a><\/p>\n<h3><strong>Location<\/strong><\/h3>\n<p>Center for Advanced Biomedical Research (CABR)<br \/>\n700 Albany St., Room W-110A<br \/>\nBoston, MA 02118<\/p>\n<p><strong>View BU Medical Campus <\/strong><a href=\"https:\/\/www.bumc.bu.edu\/about\/map-directions\/\">MAPS<\/a><\/p>\n<p><a href=\"https:\/\/www.bumc.bu.edu\/camed\/our-impact\/cores\/\">\u25c4 Back to<\/a> <strong>Core Facilities and Services<\/strong> <a href=\"https:\/\/www.bumc.bu.edu\/camed\/our-impact\/cores\/\"> website<\/a>.<\/p>\n<p style=\"text-align: right;\"><a href=\"#top\">BACK TO TOP\u2191<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Boston University Cryogenic Electron Microscopy (BU Cryo-EM) Core Facility is a custom-designed microscope facility located at the Center for Advanced Biomedical Research on the BU Medical Campus. We are committed to providing exceptional instrumentation, training, and advice for researchers looking to answer their scientific questions using cryo-EM\/cryo-ET techniques. Our goal is to provide technical [&hellip;]<\/p>\n","protected":false},"author":10364,"featured_media":0,"parent":1215,"menu_order":17,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"bu-publication":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/119721"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/users\/10364"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/comments?post=119721"}],"version-history":[{"count":29,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/119721\/revisions"}],"predecessor-version":[{"id":136684,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/119721\/revisions\/136684"}],"up":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/pages\/1215"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/media?parent=119721"}],"wp:term":[{"taxonomy":"bu-publication","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/bu-publication?post=119721"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}