{"id":134165,"date":"2025-05-19T21:00:00","date_gmt":"2025-05-20T01:00:00","guid":{"rendered":"https:\/\/www.bumc.bu.edu\/camed\/?post_type=bu-article&p=134165"},"modified":"2025-05-20T15:50:30","modified_gmt":"2025-05-20T19:50:30","slug":"study-examines-how-a-protein-called-pax3-controls-genes-in-melanoma","status":"publish","type":"bu-article","link":"https:\/\/www.bumc.bu.edu\/camed\/news-events\/articles\/2025\/study-examines-how-a-protein-called-pax3-controls-genes-in-melanoma\/","title":{"rendered":"Study Examines How a Protein Called PAX3 Controls Genes in Melanoma"},"content":{"rendered":"\t
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\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\tStudy Examines How a Protein Called PAX3 Controls Genes in Melanoma<\/strong>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/h1>\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t<\/div>\n\n\t\t\t\t<\/div>\n\n\t\t\t\n\t\t<\/div>\n\n\t\t\n\t<\/div>\n\n\t\n
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May 19, 2025<\/div>\n\t\t\n\t\t\t\t
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PAX3 is a transcription factor (proteins involved in converting DNA into RNA) that drives melanoma progression by promoting cell growth, migration and survival, while inhibiting cellular terminal differentiation, which is the final stage where a cell becomes specialized and cell division ends. However, known PAX3 target genes are limited and cannot fully explain the wide impact of PAX3 function, suggesting that there are most likely many other genes that PAX3 controls that are undiscovered.<\/p>\n\n\n\n

The PAX3 protein can regulate DNA through two separate binding domains, the paired domain (PD) and homeodomain (HD), which bind different DNA motifs<\/a>, short recurring sequences within a DNA strand that regulate gene expression. It is not clear if these two domains bind and work together to regulate genes and if they promote all or only a subset of downstream cellular events.<\/p>\n\n\n\n

A new study<\/a> by researchers at Boston University Chobanian & Avedisian School of Medicine has discovered that PAX3 mainly uses the PD to bind to the DNA, and that it mostly turns on genes\u2014many of which help cells grow and make other proteins \u2013 activities that support cancer growth.<\/p>\n\n\n\n

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Deborah Lang, PhD<\/figcaption><\/figure><\/div>\n\n\n\n

\u201cPAX3 is normally important for the development and growth of pigment cells called melanocytes, but in melanoma it is too active and helps cancer grow. What\u2019s new about our study is that we used a broad and detailed approach to see exactly where PAX3 sits on the DNA in melanoma cells \u2013 something that hasn\u2019t been done before in this way,\u201d explains corresponding author Deborah Lang<\/a>, PhD, associate professor of dermatology.<\/p>\n\n\n\n

In an effort to see where PAX3 attaches to DNA in melanoma cells, the researchers created a computer program to predict how PAX3 touches the DNA \u2013 specifically, which part of the PAX3 protein is involved. They also checked whether the genes that PAX3 touched were turned on (active) or off (repressed).<\/p>\n\n\n\n

The researchers believe that PAX3 could be a good target for treating melanoma, but currently, there are no drugs that block the function of this protein. \u201cSince we found that PAX3 works mostly through its paired domain, designing drugs that block this part of the protein could be an effective way to treat melanoma,\u201d adds Lang.<\/p>\n\n\n\n

These findings appear online<\/a> in the journal Genes.<\/p>\n","protected":false},"excerpt":{"rendered":"

Research combines bench science of protein biology, genomics with bioinformatics, and novel programs to make predictions on how a protein regulates gene expression.<\/p>\n","protected":false},"author":903,"featured_media":134213,"comment_status":"closed","ping_status":"closed","template":"","meta":{"bu_prepress_billboard":"","_bu_prepress_primary_term":"research","_bu_prepress_primary_term_manual":"Research"},"tags":[94],"bu-publication":[367],"medicine-article-category":[],"medicine-topic":[],"news-article-category":[385],"news-topic":[],"bu_edition":[390],"media_type":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/bu-article\/134165"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/bu-article"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/types\/bu-article"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/users\/903"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/comments?post=134165"}],"version-history":[{"count":7,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/bu-article\/134165\/revisions"}],"predecessor-version":[{"id":134235,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/bu-article\/134165\/revisions\/134235"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/media\/134213"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/media?parent=134165"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/tags?post=134165"},{"taxonomy":"bu-publication","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/bu-publication?post=134165"},{"taxonomy":"medicine-article-category","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/medicine-article-category?post=134165"},{"taxonomy":"medicine-topic","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/medicine-topic?post=134165"},{"taxonomy":"news-article-category","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/news-article-category?post=134165"},{"taxonomy":"news-topic","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/news-topic?post=134165"},{"taxonomy":"bu_edition","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/bu_edition?post=134165"},{"taxonomy":"media_type","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/camed\/wp-json\/wp\/v2\/media_type?post=134165"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}