NEIDL Comparative Pathology Laboratory (NCPL)
Multispectral Whole Slide imaging (mWSI)
Managed by NEIDL Comparative Pathology Laboratory (NCPL) staff. The Vectra Polaris is located within the Integrated Biomedical Imaging Services (IBIS) space in the Housman (R) building, 8th floor, 72 East Concord St., Boston. A dedicated image analysis suite with four perpetual licenses of inFORM image analysis software are conveniently located adjacent to the instrument affording multispectral unmixing of whole slide images and quantitative analysis such as positive pixel analysis, cell phenotyping, and spatial analysis.
Akoya Biosciences Vectra Polaris Whole Slide Imaging System
The addition of a Vectra Polaris Quantitative Pathology Imaging System to the BU medical campus research community includes:
- Enhanced utilization of biological specimens derived from research projects by affording the capacity of high throughput whole slide image acquisition of both brightfield and high-plex fluorescent images-up to six colors plus DAPI using the rapid acquisition MoTIF workflow.
- Increased analytical sensitivity/enhanced signal to noise ratios through multispectral unmixing of acquired images including removal of autofluorescence signals.
- Enhanced precision and objective analysis through quantitative characterization of morphomolecular signatures with spatial context afforded through an integrated image acquisition and advanced image analysis pipeline.
- Generation of continuous pathomics datasets affording correlations with other relevant biological datasets (i.e., clinicopathologic, transcriptomics, etc.) enabling for more rigorous and integrated research approaches to further elucidate clinicopathologic correlates of disease.
Akoya Biosciences Vectra Polaris Specifications
|Slide Capacity||80+ (continuous loading)|
|Output File Format||.qptiff and .im3|
|Objectives||5, 10, 20, 40x|
|Image Acquisition||Brightfield and 9-channel fluorescence-30 minutes to an hour|
|InFORM Image Analysis Features||Tissue segmentation (machine learning), cell segmentation, phenotyping (machine learning), scoring, spatial analysis|
|Liquid Crystal Tunable Filter||Yes|
|Multispectral Imaging with Unmixing||Yes|
|Integrated Spectral Unmixing||Yes|
|Autofluorescence Removal||Yes: Quantitation and isolation using automatically selected AF filter|
|Synthetic Spectra for Unmixing||Yes|
|Multiband Epi-Fluorescence Filters||Yes|
|Flash LEDs for Photobleaching Protection||Yes|
|Local Support Team||Sales Specialist/Application Scientists (PhD)/Service Engineer (PhD)/Bioinformatics Scientist (PhD)|
Vectra Polaris Epi-fluorescence Filter Cube Characteristics
|Slot||Cube||Band||Excitation (nm)||Emission (nm)|
|Cut on||Cut off||Cut on||Cut off|
|4||Texas Red||Texas Red||567||589||604||710|
|9||Sample AF||Sample AF|
Recommended Fluorescent Dyes
|Akoya Opal Dyes||Alexa/ATTO Dyes||Biotium CF® Dyes||Cyanine Dyes||Other|
|Opal 620||AF594||CF594||Cy3,5||Texas Red|
|Highlighted dyes are part of the MOTiF rapid 7-color acquisition protocol and recommended for maximum efficiency and quality of whole-slide imaging.|
Services provided by staff include:
- Core competency training on the operation of the Vectra Polaris image acquisition.
- Assisted or independent use of the Vectra Polaris whole slide scanner, with the latter only available to those approved by NCPL staff.
- Guidance in generating multispectral libraries to remove auto-fluorescence and maximize signal to noise ratios of images using inForm.
- Assistance and training in the generation of quantitative pathology outputs using inForm.
||BU Internal Rates||External Rates|
How to Schedule
Usage and Training
Training is mandatory for all new users and requires:
- Scheduling of two consecutive 1-hour sessions of “Assisted Use” for practical in-person training.
- Users are expected to bring their own slides for scanning during the training session.
- A combination of both brightfield and fluorescent slides is recommended to familiarize users with developing scan profiles for each of these formats.
Please contact us to schedule training or assisted use. Once users have been trained, time on the instruments may be scheduled.
New users should watch these short videos prior to attending in-person training held by core staff:
- How to power cycle the instrument.
- How to load slides into carries.
- How to load carriers into the instrument.
- Polaris software overview which shows you how to scan slides, take references and acquire spectra from library slides.
- inForm analysis covers the basic of image analysis using inForm.
We require that all users cancel sessions 24 hours before the scheduled start time. Failure to cancel will result in the user (or PI) being billed for the entire scheduled duration.
The Vectra Polaris was funded by the NIH Award Number S10OD030269. We greatly appreciate acknowledgement of NIH S10OD030269 in publications when authors use our equipment and/or assistance in their research.
Nicholas Crossland, DVM, DACVP
(617) 358-9285 | firstname.lastname@example.org
Hans Gertje, BS, HTL(ASCP)CMQIHCCM
NCPL Lab Supervisor
(617) 358-9139 | email@example.com
Boston University Chobanian & Avedisian School of Medicine
Housman Medical Research Center
72 East Concord Street, R Building, R-824A
Boston, MA 02118