Expertise includes: Molecular biology in general including cloning, genomics, RTPCR, geneomic PCR, vector construction including Lentiviral vectors, RNA and DNA isolation animal work, creation of mouse IPSC cells and infection with Lentiviral vectors, transgenic mice, teratoma creation and mouse tumors. Antibody background and antibody genomic families. Previously have worked on breast cancer involving the gene BCAR3 and related family members as related to estrogen treatment resistance, differentiation patterns, cell migration, cell matrix interactions and Cancer Stem cells and metastases. I also created a BCAR KO mouse strain with unusual characteristics. Previous studied also were applied to signal transduction in cancer; Had interest in Zinc finger proteins and RNA zipcodes.
More recently in conjunction with the company ANTIGEN, we have interests in AIDs with a SBIR grant. Further we have interests in immunology related to Antibody Dependent Cell Cytotoxicity ADCC. We have further interests in Antibody FCR (receptors) and have recent research in COVID. We have created unusual cloning vectors and cell strains that do not fucosilate proteins which may
allow antibodies greater stability (as well as other proteins). We also work with single-chain antibodies and express high amounts of proteins in variants of CHO cell lines.
- Research Assistant Professor, Hematology & Medical Oncology, Medicine, Boston University School of Medicine
- Member, BU-BMC Cancer Center, Boston University
- Member, Genome Science Institute, Boston University
- University of Washington, PhD
- University of Washington, MS
- University of California, Irvine, BS
- University of California, Irvine, BA
- Published on 10/10/2016
Changchuin Mao, Richard Near and Wenda Gao. Identification of a Guinea Pig Fc?Receptor that Exhibits Enhanced Binding to Afucosylated Human and Mouse IgG. . J Infect Dis Med1: 102-105. . 2016; 102-105. .
- Published on 6/18/2012
Makkinje A, Vanden Borre P, Near RI, Patel PS, Lerner A. Breast cancer anti-estrogen resistance 3 (BCAR3) protein augments binding of the c-Src SH3 domain to Crk-associated substrate (p130cas). J Biol Chem. 2012 Aug 10; 287(33):27703-14. PMID: 22711540.
- Published on 1/22/2011
Vanden Borre P, Near RI, Makkinje A, Mostoslavsky G, Lerner A. BCAR3/AND-34 can signal independent of complex formation with CAS family members or the presence of p130Cas. Cell Signal. 2011 Jun; 23(6):1030-40. PMID: 21262352.
- Published on 5/18/2009
Makkinje A, Near RI, Infusini G, Vanden Borre P, Bloom A, Cai D, Costello CE, Lerner A. AND-34/BCAR3 regulates adhesion-dependent p130Cas serine phosphorylation and breast cancer cell growth pattern. Cell Signal. 2009 Sep; 21(9):1423-35. PMID: 19454314.
- Published on 4/3/2009
Near RI, Smith RS, Toselli PA, Freddo TF, Bloom AB, Vanden Borre P, Seldin DC, Lerner A. Loss of AND-34/BCAR3 expression in mice results in rupture of the adult lens. Mol Vis. 2009; 15:685-99. PMID: 19365570.
- Published on 9/1/2007
Near RI, Zhang Y, Makkinje A, Vanden Borre P, Lerner A. AND-34/BCAR3 differs from other NSP homologs in induction of anti-estrogen resistance, cyclin D1 promoter activation and altered breast cancer cell morphology. J Cell Physiol. 2007 Sep; 212(3):655-65. PMID: 17427198.
- Published on 1/1/2005
Felekkis KN, Narsimhan RP, Near R, Castro AF, Zheng Y, Quilliam LA, Lerner A. AND-34 activates phosphatidylinositol 3-kinase and induces anti-estrogen resistance in a SH2 and GDP exchange factor-like domain-dependent manner. Mol Cancer Res. 2005 Jan; 3(1):32-41. PMID: 15671247.
- Published on 10/15/2003
Cai D, Iyer A, Felekkis KN, Near RI, Luo Z, Chernoff J, Albanese C, Pestell RG, Lerner A. AND-34/BCAR3, a GDP exchange factor whose overexpression confers antiestrogen resistance, activates Rac, PAK1, and the cyclin D1 promoter. Cancer Res. 2003 Oct 15; 63(20):6802-8. PMID: 14583477.
- Published on 1/15/2003
Cai D, Felekkis KN, Near RI, O'Neill GM, van Seventer JM, Golemis EA, Lerner A. The GDP exchange factor AND-34 is expressed in B cells, associates with HEF1, and activates Cdc42. J Immunol. 2003 Jan 15; 170(2):969-78. PMID: 12517963.
- Published on 2/1/2002
Moon E, Lee R, Near R, Weintraub L, Wolda S, Lerner A. Inhibition of PDE3B augments PDE4 inhibitor-induced apoptosis in a subset of patients with chronic lymphocytic leukemia. Clin Cancer Res. 2002 Feb; 8(2):589-95. PMID: 11839681.
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