Our laboratory is pleased to provide facilities to investigators of Boston University Medical Campus and Boston VA Medical Center in clinic application of proteomics with expert, accurate, and collaborative interactions. These include i) Fluorescence-assistant Autoimmune Display (FaAD) to identify autoimmune responses against tumors, infectious diseases and autoimmune diseases, ii) Differential Gel Electrophoresis (DiGE) to identify differentially expressed proteins in blood/tissue samples, iii) Fluorescence-assistant Multi-dimensional HPLC (FaMD-HPLC) to differentially profile protein expression in patient biopsies, iv) LC-MALDI-TOF and nanoLC-Q-TOF (LC-MS/MS) for protein identification, protein profiling, and marker discovery, and v) Liquid protein array (xMAP from Luminex) to validate protein markers in patients.
We are studying the biological function of dsRNA and dsRNA-binding proteins. These include the dsRNA editing enzyme ADAR1, ADAR2 and ADAR3. ADAR1 is ubiquitously expressed particularly in immune organs; ADAR2 is found in all tissues but dominant in neuronal system; and ADAR3 is exclusively in the brain. Currently our interest is focused on the function of ADAR1 in viral infection/stress, and ADAR2 in traumatic stress.
Another focus of our laboratory is to identify the tumor specific complement activator (tsCA). This factor is found to activate human complement to kill different tumor cells in vitro.
- VA Boston Healthcare System
- Université de Montréal, PhD
- Beijing University of Chemical Technology, MSc
- Sichuan University, BSc
- Published on 11/1/2006
Nie Y, Hammond GL, Yang JH. Double-stranded RNA deaminase ADAR1 increases host susceptibility to virus infection. J Virol. 2007 Jan; 81(2):917-23. PMID: 17079286.
- Published on 8/1/2005
Nie Y, Ding L, Kao PN, Braun R, Yang JH. ADAR1 interacts with NF90 through double-stranded RNA and regulates NF90-mediated gene expression independently of RNA editing. Mol Cell Biol. 2005 Aug; 25(16):6956-63. PMID: 16055709.
- Published on 11/2/2004
Xia S, Yang J, Su Y, Qian J, Ma E, Haddad GG. Identification of new targets of Drosophila pre-mRNA adenosine deaminase. Physiol Genomics. 2005 Jan 20; 20(2):195-202. PMID: 15522950.
- Published on 1/7/2004
Nie Y, Zhao Q, Su Y, Yang JH. Subcellular distribution of ADAR1 isoforms is synergistically determined by three nuclear discrimination signals and a regulatory motif. J Biol Chem. 2004 Mar 26; 279(13):13249-55. PMID: 14711814.
- Published on 9/3/2003
Yang JH, Nie Y, Zhao Q, Su Y, Pypaert M, Su H, Rabinovici R. Intracellular localization of differentially regulated RNA-specific adenosine deaminase isoforms in inflammation. J Biol Chem. 2003 Nov 14; 278(46):45833-42. PMID: 12954622.
- Published on 9/1/2003
Nie Y, Wu K, Yang J, Tian F, Li L, Chen B, Fan D. Induction of T lymphocytes specific to human gastric cancer using HLA-A matched allogeneic gastric tumor cells. J Immunother. 2003 Sep-Oct; 26(5):403-11. PMID: 12973029.
- Published on 5/1/2003
Yang JH, Luo X, Nie Y, Su Y, Zhao Q, Kabir K, Zhang D, Rabinovici R. Widespread inosine-containing mRNA in lymphocytes regulated by ADAR1 in response to inflammation. Immunology. 2003 May; 109(1):15-23. PMID: 12709013.
- Published on 1/1/2003
Wu J, Shi YQ, Wu KC, Zhang DX, Yang JH, Fan DM. Angiostatin up-regulation in gastric cancer cell SGC7901 inhibits tumorigenesis in nude mice. World J Gastroenterol. 2003 Jan; 9(1):59-64. PMID: 12508352.
- Published on 9/1/2002
Rabinovici R, Zhang D, Su Y, Luo X, Zhao Q, Yang JH. MOB-1 and TNF-alpha interact to induce microvascular lung injury. Shock. 2002 Sep; 18(3):261-4. PMID: 12353928.
- Published on 4/1/2002
Kabir K, Gelinas JP, Chen M, Chen D, Zhang D, Luo X, Yang JH, Carter D, Rabinovici R. Characterization of a murine model of endotoxin-induced acute lung injury. Shock. 2002 Apr; 17(4):300-3. PMID: 11954830.
View 8 more publications: View full profile at BUMC