{"id":17260,"date":"2021-12-09T14:34:21","date_gmt":"2021-12-09T19:34:21","guid":{"rendered":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/?post_type=profile&p=17260"},"modified":"2023-05-15T13:48:10","modified_gmt":"2023-05-15T17:48:10","slug":"dmitry-kretov","status":"publish","type":"profile","link":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/profiles\/dmitry-kretov\/","title":{"rendered":"Dmitry Kretov"},"content":{"rendered":"

In the laboratory of Dr. Daniel Cifuentes, I am trying to uncover the mechanisms of post-transcriptional regulation of gene expression during vertebrate development. Specifically, my efforts are devoted to elucidating how microRNAs are produced, which set of targets they regulate, and what impact this regulation has on the cell differentiation process.<\/span><\/p>\n

I initially discovered an interest in post-transcriptional control while working on my Master\u2019s thesis project at the Institute of Protein Research of the Russian Academy of Sciences in the laboratory of Dr. Lev Ovchinnikov whose focus was on understanding how RNA-binding proteins (RBPs) control the translation of mRNA. I continued to pursue my passion for RBPs during my Ph.D. at the University of Evry in France (part of the University of Paris-Saclay) under the mentorship of Drs. Patrick Curmi and David Pastr\u00e9 with continued support by Dr. Ovchinnikov. During this time, I applied methods of biochemistry and structural biology to investigate the organization of messenger ribonucleoprotein complexes (mRNPs) formed by different RBPs and mRNAs in vitro.<\/span><\/p>\n

In the Cifuentes laboratory, I use zebrafish (Danio rerio) as a model organism to understand the regulatory mechanisms responsible for miRNA processing and accumulation during erythropoiesis and the contribution of microRNAs to cell fate commitment during the formation of blood cells. In a more recent project, I am exploring the principles governing the recognition of mRNA targets by RBPs during early zebrafish development and how these interactions contribute to the dynamic changes in gene expression taking place during the maternal-to-zygotic transition.<\/span><\/p>\n

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Publications<\/h4>
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1. Hekman RM*, Hume AJ*, Goel RK*, Abo KM*, Huang J*, Blum BC*, Werder RB*, Suder EL*, Paul I*, Phanse S, Youssef A, Alysandratos KD, Padhorny D, Ojha S, Mora-Martin A, Kretov D, Ash PEA, Verma M, Zhao J, Patten JJ, Villacorta-Martin C, Bolzan D, Perea-Resa C, Bullitt E, Hinds A, Tilston-Lunel A, Varelas X, Farhangmehr S, Braunschweig U, Kwan JH, McComb M, Basu A, Saeed M, Perissi V, Burks EJ, Layne MD, Connor JH, Davey R, Cheng JX, Wolozin BL, Blencowe BJ, Wuchty S, Lyons SM, Kozakov D, Cifuentes D, Blower M, Kotton DN, Wilson AA, M\u00fchlberger E, Emili A. Actionable Cytopathogenic Host Responses of Human Alveolar Type 2 Cells to SARS-CoV-2. Molecular Cell. 2021 Jan 07; 81(1):212.<\/span>
\n2. Kretov DA, Walawalkar IA, Mora-Martin A, Shafik AM, Moxon S, Cifuentes D. (2020) Ago2-Dependent Processing Allows miR-451 to Evade the Global MicroRNA Turnover Elicited During Erythropoiesis. Molecular Cell, 78(2):317-328.e6.<\/span>
\n3. Kretov DA*, Mordovkina DA*, Eliseeva IA, Lyabin DN, Polyakov DN, Joshi V, Desforges B, Hamon L, Lavrik OI, Pastr\u00e9 D, Curmi PA, & Ovchinnikov LP. (2019) Inhibition of Transcription Induces Phosphorylation of YB-1 at Ser102 and Its Accumulation in the Nucleus. Cells, 9(1).<\/span>
\n4. Kretov DA*, Cl\u00e9ment MJ*, Lambert G*, Durand D, Lyabin DN, Bollot G, Bauvais C, Samsonova A, Budkina K, Maroun RC, Hamon L, Bouhss A, Lescop E, Toma F, Curmi PA, Maucuer A, Ovchinnikov LP, Pastr\u00e9 D. (2019) YB-1, an abundant core mRNA-binding protein has the capacity to form an RNA nucleoprotein filament: a structural analysis. Nucleic Acids Research, 47(6):3127-3141.<\/span>
\n5. Marivin A, Morozova V, Walawalkar I, Leyme A, Kretov DA, Cifuentes D, Dominguez I, Garcia-Marcos M. (2019) GPCR-independent activation of G proteins promotes apical cell constriction in vivo. Journal of Cell Biology, 218(5):1743-1763.<\/span>
\n6. Maucuer A, Desforges B, Joshi V, Boca M, Kretov DA, Hamon L, Bouhss A, Curmi PA, Pastr\u00e9 D. (2018) Microtubules as platforms for probing liquid-liquid phase separation in cells – application to RNA-binding proteins. Journal of Cell Science, 131(11).<\/span>
\n7. Kretov DA, Shafik AM, Cifuentes D. (2018) Assessing miR-451 Activity and Its Role in Erythropoiesis. Methods in Molecular Biology, 1680:179-190.<\/span>
\n8. Abrakhi S, Kretov DA, Desforges B, Dobra I, Bouhss A, Pastr\u00e9 D, Hamon L. (2017) Nanoscale Analysis Reveals the Maturation of Neurodegeneration-Associated Protein Aggregates: Grown in mRNA Granules Then Released by Stress Granule Proteins. ACS Nano, 11(7):7189-7200.<\/span>
\n9. Kretov DA, Curmi PA, Hamon L, Abrakhi S, Desforges B, Ovchinnikov LP, Pastr\u00e9 D. (2015) mRNA and DNA selection via protein multimerization: YB-1 as a case study. Nucleic Acids Research, 43(19):9457-73.<\/span>
\n10. Boca M, Kretov DA, Desforges B, Mephon-Gaspard A, Curmi PA, Pastr\u00e9 D. (2015) Probing protein interactions in living mammalian cells on a microtubule bench. Scientific Reports, 5:17304.<\/span><\/p>\n

*denotes equal contribution<\/span><\/p>\n

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