{"id":18238,"date":"2023-05-04T14:22:26","date_gmt":"2023-05-04T18:22:26","guid":{"rendered":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/?p=18238"},"modified":"2026-01-07T15:12:39","modified_gmt":"2026-01-07T20:12:39","slug":"new-publication-the-saeed-lab-publishes-a-paper-reporting-cell-culture-systems-for-isolation-of-sars-cov-2-from-clinical-isolates-and-production-of-recombinant-virus","status":"publish","type":"post","link":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/2023\/05\/04\/new-publication-the-saeed-lab-publishes-a-paper-reporting-cell-culture-systems-for-isolation-of-sars-cov-2-from-clinical-isolates-and-production-of-recombinant-virus\/","title":{"rendered":"New Publication: The Saeed lab publishes a paper reporting cell culture systems for isolation of SARS-CoV-2 from clinical isolates and production of recombinant virus"},"content":{"rendered":"

The Saeed lab publishes a paper reporting cell culture systems for isolation of SARS-CoV-2 from clinical isolates and production of recombinant virus.<\/p>\n

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Summary:<\/strong> A simple and robust cell culture system is essential for generating authentic SARS-CoV-2 stocks for evaluation of viral pathogenicity, screening of antiviral compounds, and preparation of inactivated vaccines. Evidence suggests that Vero E6, a cell line commonly used in the field to grow SARS-CoV-2, does not support efficient propagation of new viral variants and triggers rapid cell culture adaptation of the virus. We generated a panel of 17 human cell lines overexpressing SARS-CoV-2 entry factors and tested their ability to support viral infection. Two cell lines, Caco-2\/AT and HuH-6\/AT, demonstrated exceptional susceptibility, yielding highly concentrated virus stocks. Notably, these cell lines were more sensitive than Vero E6 cells in recovering SARS-CoV-2 from clinical specimens. Further, Caco-2\/AT cells provided a robust platform for producing genetically reliable recombinant SARS-CoV-2 through a reverse genetics system. These cellular models are a valuable tool for the study of SARS-CoV-2 and its continuously emerging variants.<\/span><\/p>\n

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Find the full article here.<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

The Saeed lab publishes a paper reporting cell culture systems for isolation of SARS-CoV-2 from clinical isolates and production of […]<\/p>\n","protected":false},"author":8106,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[104,4660],"tags":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/posts\/18238"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/users\/8106"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/comments?post=18238"}],"version-history":[{"count":2,"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/posts\/18238\/revisions"}],"predecessor-version":[{"id":18242,"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/posts\/18238\/revisions\/18242"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/media?parent=18238"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/categories?post=18238"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/tags?post=18238"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}