{"id":15838,"date":"2020-07-07T13:26:36","date_gmt":"2020-07-07T17:26:36","guid":{"rendered":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/?p=15838"},"modified":"2026-01-07T15:13:55","modified_gmt":"2026-01-07T20:13:55","slug":"research-discoveries-seeing-g-protein-signaling","status":"publish","type":"post","link":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/2020\/07\/07\/research-discoveries-seeing-g-protein-signaling\/","title":{"rendered":"Research discoveries: seeing G-protein signaling"},"content":{"rendered":"<div>The Garcia-Marcos lab has developed a suite of modular optical biosensors that enables detection of hetrotrimeric G-protein activity in live cells in contexts ranging from tumor cells to primary neurons.<a href=\"https:\/\/www.bumc.bu.edu\/biochemcellbio\/2020\/07\/07\/research-discoveries-seeing-g-protein-signaling\/graphical-abstract-website\/\" rel=\"attachment wp-att-15846\"><img loading=\"lazy\" src=\"\/biochemcellbio\/files\/2020\/07\/Graphical-abstract-website.tif\" alt=\"\" width=\"1\" height=\"1\" class=\"alignright size-thumbnail wp-image-15846\" \/><\/a><\/div>\n<div><span><a href=\"https:\/\/www.bumc.bu.edu\/biochemcellbio\/2020\/07\/07\/research-discoveries-seeing-g-protein-signaling\/screen-shot-2020-07-07-at-1-53-20-pm\/\" rel=\"attachment wp-att-15847\"><img loading=\"lazy\" src=\"\/biochemcellbio\/files\/2020\/07\/Screen-Shot-2020-07-07-at-1.53.20-PM-328x300.png\" alt=\"\" width=\"328\" height=\"300\" class=\"alignright size-medium wp-image-15847\" srcset=\"https:\/\/www.bumc.bu.edu\/biochemcellbio\/files\/2020\/07\/Screen-Shot-2020-07-07-at-1.53.20-PM-328x300.png 328w, https:\/\/www.bumc.bu.edu\/biochemcellbio\/files\/2020\/07\/Screen-Shot-2020-07-07-at-1.53.20-PM-768x702.png 768w, https:\/\/www.bumc.bu.edu\/biochemcellbio\/files\/2020\/07\/Screen-Shot-2020-07-07-at-1.53.20-PM.png 856w\" sizes=\"(max-width: 328px) 100vw, 328px\" \/><\/a>\u00a0<\/span><\/div>\n<div><span>Heterotrimeric G-proteins are the main transducers of signals from GPCRs, mediating the action of\u00a0<\/span>countless natural stimuli and therapeutic agents (about one-third of FDA-approved drugs). The new biosensors described in the paper published in <a href=\"https:\/\/www.cell.com\/cell\/fulltext\/S0092-8674(20)30752-2\"><i>Cell<\/i><\/a> allow the direct detection of\u00a0endogenous active G-proteins, something that had not been possible to date. This work showcases several biosensors for different G-protein subtypes and applications\u00a0in multiple cellular models and research areas, from characterizing cancer-associated G-protein mutants to neurotransmitter signaling in primary neurons.\u00a0This new resource will be valuable in characterizing\u00a0G-protein signaling in live cells with\u00a0high fidelity, temporal resolution, and convenience, propelling both fundamental research and drug discovery applications.<\/div>\n","protected":false},"excerpt":{"rendered":"<p>The Garcia-Marcos lab has developed a suite of modular optical biosensors that enables detection of hetrotrimeric G-protein activity in live [&hellip;]<\/p>\n","protected":false},"author":1191,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[104,4654,100],"tags":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/posts\/15838"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/users\/1191"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/comments?post=15838"}],"version-history":[{"count":3,"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/posts\/15838\/revisions"}],"predecessor-version":[{"id":15848,"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/posts\/15838\/revisions\/15848"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/media?parent=15838"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/categories?post=15838"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/biochemcellbio\/wp-json\/wp\/v2\/tags?post=15838"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}