{"id":9877,"date":"2021-10-20T15:16:19","date_gmt":"2021-10-20T19:16:19","guid":{"rendered":"https:\/\/www.bumc.bu.edu\/anatneuro\/?page_id=9877"},"modified":"2025-05-06T11:55:40","modified_gmt":"2025-05-06T15:55:40","slug":"ella-zeldich-lab","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/anatneuro\/ella-zeldich-lab\/","title":{"rendered":"Ella Zeldich Lab"},"content":{"rendered":"<\/div>\n<p><!-- ___________________Navbar___________________ --><\/p>\n<div id=\"navvy\"><\/div>\n<p><script type=\"text\/javascript\" src=\"\/anatneuro\/files\/2025\/05\/nav-5-2025.js\"><\/script><\/p>\n<p><!-- ___________________Banner___________________ --><\/p>\n<div class=\"mobile-title mobile-banner\">\n<p class=\"mobile-header\">Laboratory of Molecular Signaling in Neurological Disorders (Zeldich Lab)<\/p>\n<\/div>\n<style>.mobile-banner{background-image: url(\"\/anatneuro\/files\/2021\/10\/Image20-40xeuploid-4-weeks.jpg\"); background-size: cover;}<\/style>\n<div class=\"blur\">\n<div class=\"zeldich-parallax\">\n<div class=\"true-nav nav-border\"><\/div>\n<div class=\"title-padding\">\n<p style=\"padding-top: 0px;\" class=\"title-header\">Laboratory of Molecular Signaling in Neurological Disorders (Zeldich Lab)<\/p>\n<\/div>\n<\/div>\n<p><!-- ___________________Lab Details___________________ --><\/p>\n<div class=\"row\">\n<div class=\"col-md-7\">\n<div class=\"universal-pad\" style=\"margin-left: 2vw;\">\n<h2>Our Research<\/h2>\n<p>Our\u00a0lab\u00a0is focusing on studying the cellular and molecular machinery mediating the connection<br \/>\nbetween Down Syndrome and Alzheimer\u2019s disease.\u00a0 We are utilizing novel and powerful 3D<br \/>\ncellular models (organoids and assembloids) derived from human induced pluripotent stem cells<br \/>\n(iPSCs) to identify molecular determinants underlying the changes in a cell lineage commitment,<br \/>\ndevelopment, maturation, and functionality in Down Syndrome.\u00a0 The main goal of our research<br \/>\nis to understand the crucial alterations leading to cellular dysfunction in trisomy 21 to identify<br \/>\npotential therapeutic targets and develop ameliorative strategies.<\/p>\n<p>In addition, we are actively collaborating with Drs. Luebke, Medalla, and Moore, to investigate<br \/>\nthe molecular mechanisms\u00a0that are responsible for the differences in cellular and synaptic<br \/>\nproperties of cortical networks in physiological and age-related\u00a0processes.<\/p>\n<p>Our\u00a0lab\u00a0utilizes a combination of cutting-edge cellular and molecular approaches, such as<br \/>\nsingle-cell transcriptome profiling, ATAC-seq, Patch-seq, CRISPR, immuno-labeling, confocal<br \/>\nmicroscopy, and more.<\/p>\n<\/div>\n<\/div>\n<div class=\"col-md-5\">\n<img src=\"\/anatneuro\/files\/2021\/10\/A-picture-to-put-near-the-introduction-about-the-lab-copy.jpg\" style=\"max-width: 488px; max-height: 488px;\" class=\"info-img\">\n<\/div>\n<\/div>\n<p><!-- ___________________Buttons___________________ --><\/p>\n<div class=\"row fade\" style=\"margin: auto; padding-top: 30px; padding-bottom: 30px;\">\n<div class=\"col-lg-4\">\n<div class=\"button2\" style=\"margin: auto; margin-top: 20px; margin-bottom: 20px;\" onclick=\"document.body.classList.add('active')\">\n<p><a class=\"box-shade\" style=\"background-color: rgba(0,0,0,0);\"><\/a><br \/>\n<img style=\" width: 440px; height: 240px; object-fit: cover;\" src=\"\/anatneuro\/files\/2021\/10\/Background-for-panel-1.jpg\" alt=\"\"><br \/>\n<span style=\"z-index: 3; color: #ffffff; font-weight: normal; position: absolute;\" class=\"button-text\">Epigenetic and transcriptomic dysregulation as a common denominator in Down Syndome (DS) and DS-associated Alzheimer\u2019s Disease (DS-AD).<\/span><\/p>\n<div class=\"button-backgrounds\"><\/div>\n<\/div>\n<\/div>\n<div class=\"col-lg-4\">\n<div class=\"button2\" style=\"margin: auto; margin-top: 20px; margin-bottom: 20px;\" onclick=\"document.body.classList.add('activeC')\">\n<p><a class=\"box-shade\" style=\"background-color: rgba(0,0,0,0);\"><\/a><br \/>\n<img style=\" width: 440px; height: 240px; object-fit: cover;\" src=\"\/anatneuro\/files\/2023\/10\/Am-image-for-Panel-2.png\" alt=\"\"><br \/>\n<span style=\"color: #ffffff; position: absolute; z-index: 3; font-weight: normal; margin: 20px;\" class=\"button-text\">Understanding neuronal dysfunction in Down Syndrome using cortical organoids.<\/span><\/p>\n<div class=\"button-backgrounds2\"><\/div>\n<\/div>\n<\/div>\n<div class=\"col-lg-4\">\n<div class=\"button2\" style=\"margin: auto; margin-top: 20px; margin-bottom: 20px;\" onclick=\"document.body.classList.add('active2')\">\n<p><a class=\"box-shade\"  style=\"background-color: rgba(0,0,0,0);\"><\/a><br \/>\n<img style=\" width: 440px; height: 240px; object-fit: cover;\" src=\"\/anatneuro\/files\/2021\/10\/an-alertnative-background-for-panel-3.png\" alt=\"\"><br \/>\n<span style=\"color: #000000; position: absolute; z-index: 3; font-weight: normal; letter-spacing: 1px;\" class=\"button-text\">Single cell transcriptomic profiling of cortical neurons<br \/>\n<\/span><\/p>\n<div class=\"button-backgrounds2\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p><!-- ___________________Popup 1___________________ --><\/p>\n<div class=\"wrapper1\">\n<div class=\"popup\">\n<div class=\"popup-inside\">\n<div class=\"backgrounds\">\n<div class=\"background\"><\/div>\n<div class=\"background background2\"><\/div>\n<div class=\"background background3\"><\/div>\n<div class=\"background background4\"><\/div>\n<div class=\"background background5\"><\/div>\n<div class=\"background background6\"><\/div>\n<\/div>\n<\/div>\n<div class=\"content1\">\n<div class=\"content1-wrapper\">\n<p style=\"text-align: right; margin-top: -50px; margin-right: -50px;\" onclick=\"document.body.classList.remove('active')\"><i class=\"close fa fa-times fa-4x\"><\/i><\/p>\n<div class=\"p-content\">\n<h2 class=\"pop\"><a class=\"news\" href=\"#\">Epigenetic and transcriptomic dysregulation as a common denominator in Down<br \/>\nSyndome (DS) and DS-associated Alzheimer\u2019s Disease (DS-AD).<\/h2>\n<p><\/a><\/p>\n<p>DS is caused by the triplication of human chromosome 21 (HSA21) and results in<br \/>\nintellectual disability and early onset of AD. Studies in the DS brain uncovered abnormal fetal<br \/>\nbrain development, disruptions in myelin production, and reduced neuronal connectivity.<\/p>\n<p>Human three-dimensional, region-specific organoid systems derived from induced<br \/>\npluripotent stem cells (iPSCs) present a biologically relevant model for DS-AD and provide<br \/>\nunprecedented opportunities to investigate human brain pathological processes in vitro. Myelin<br \/>\ndisruption is an early pathological feature of Alzheimer\u2019s Disease (AD) that often precedes the<br \/>\nformation of amyloid beta (A\u03b2) plaques and neurofibrillary tangles within the cerebral cortex.<br \/>\nDelayed oligodendrocyte maturation, and disruptions in myelin production have been found in<br \/>\nindividuals with DS and DS-mouse models, contributing potentially to the predisposition of DS<br \/>\nbrain to AD pathology and altering neuronal connectivity. While the triplicated HSA21 genes,<br \/>\nsuch as Olig1, Olig2, and S100B have been implicated in abnormal glial biology, the gene<br \/>\ndosage effect of other HSA21 genes, such as epigenetic modulators that can contribute further<br \/>\nto the abnormal development of oligodendrocytes, deficient myelination, and altered<br \/>\ncommunication between neuronal and glia cells.<\/p>\n<p><img src=\"\/anatneuro\/files\/2023\/10\/P1I1.jpg\" alt=\"\"\/><\/p>\n<p>&#8211; Using DS-derived COs, enriched with oligodendrocytes we demonstrated an abnormal<br \/>\ncommitment and differentiation of oligodendrocyte lineage in<br \/>\ntrisomy and detected a deficient expression of myelin-related<br \/>\nmarkers, such as MBP and BCAS1, observed in actively<br \/>\nmyelinating oligodendrocytes in trisomic CO (Fig.1). Using this<br \/>\nsystem, we are now able to fully characterize the effect of trisomy<br \/>\n21 on oligodendrocyte development across different stages of differentiation.<br \/>\nFurthermore, we are currently investigating the correlation<br \/>\nbetween alterations in the epigenetic landscape and changes in gene expression leading to the<br \/>\nabnormal temporal development of oligodendrocytes and neurons in COs using a newly<br \/>\ndeveloped Multiome ATAC + Gene Expression platform<\/p>\n<p>&#8211; In our previous single-cell RNA sequencing (scRNA-seq) analysis of euploid and<br \/>\ntrisomic COs, we identified a cluster of X-linked genes containing brain-expressed X-<br \/>\nlinked\/transcription elongation factor A (SII) (BEX\/TCEAL) family members, that is<br \/>\ndownregulated in trisomy. At the same time, trisomic cells showed a significantly increased<br \/>\nexpression of non-coding X-inactive specific transcript (XIST), responsible for the X-<br \/>\nchromosome inactivation. Noticeably, other recent studies, including those performed in DS cell<br \/>\nlines and human postmortem brains, showed downregulated expression of different genes in<br \/>\nthis cluster accompanied by enhanced XIST levels. However, the aberrant expression of these<br \/>\ntranscription factors and their potential connection to brain pathology in trisomy has been<br \/>\nneglected. Herein, our current studies are aimed to causatively link the dysregulation of XIST<br \/>\nand X chromosome-linked genes to abnormal brain development and AD pathology in DS.<br \/>\n<br \/>Our next goals:<\/p>\n<p style=\"margin-left: 8px;\">&#8211; To understand the role of HMGN1, the HSA21 gene, a nucleosome remodeler in the<br \/>\nspecific transcriptomic changes in gene expression leading to the abnormal lineage<br \/>\ndevelopment and asynchronous maturation of neuronal and glia cells.<\/p>\n<p>In our previous studies using regionally-patterned cortical organoids (COs) resembling<br \/>\ndorsal forebrain generated from patient-derived isogenic iPSC lines, we identified phenotypic<br \/>\nand transcriptomic changes in excitatory neuronal populations and detected increased amyloid<br \/>\n\u03b2 deposition and hyperphosphorylated tau in trisomic COs. We expanded our experimental<br \/>\nmodel to include both excitatory and inhibitory neurons as well as oligodendrocytes and<br \/>\nincorporated ventrally derived COs (vCOs), resembling medial ganglionic eminence (MGE)<br \/>\ngiving rise to inhibitory GABAergic neurons and oligodendrocytes. Euploid and trisomic dCOs<br \/>\nand vCOs can be separately profiled at cellular and functional levels and can be fused in vitro to<br \/>\ngenerate forebrain assembloids to recapitulate the migration of oligodendrocytes and<br \/>\ninterneurons as well as the interactions of GABAergic and glutamatergic neurons.<\/p>\n<p>Utilizing human isogenic iPSC lines enables us to isolate the effects driven by trisomy and find<br \/>\ncommon dysregulation patterns that reflect more general neuronal deficits underlying IDs and<br \/>\nDS-AD pathology.<\/p>\n<p>Neuronal network activity is derived from concerted firing of interconnected excitatory<br \/>\nand inhibitory neurons, which arise from distinct neurogenic pools that are not fully represented<br \/>\nin our dorsally-derived COs (dCOs), which contain mostly excitatory neurons. Thus, we<br \/>\nexpanded our experimental model to include both excitatory and inhibitory neurons and<br \/>\nincorporated ventrally derived COs (vCOs), resembling medial ganglionic eminence (MGE)<br \/>\ngiving rise to inhibitory GABAergic neurons 7,8 . Euploid and trisomic dCOs and vCOs can be<br \/>\nseparately profiled at cellular and functional levels and can be fused in vitro to generate<br \/>\nforebrain assembloids (FAs) to recapitulate cell migration and interactions of GABAergic and<br \/>\nglutamatergic neurons and network assembly observed in vivo<\/p>\n<p>Myelin disruption is an early pathological feature of Alzheimer\u2019s Disease (AD) that often<br \/>\nprecedes the formation of amyloid beta (A\u03b2) plaques and neurofibrillary tangles within the<br \/>\ncerebral cortex. Delayed oligodendrocyte maturation, and disruptions in myelin production have<br \/>\nbeen found in individuals with DS and DS-mouse models, contributing potentially to the<br \/>\npredisposition of DS brain to AD pathology and altering neuronal connectivity. While the<br \/>\ntriplicated HSA21 genes, such as Olig1, Olig2, and S100B have been implicated in abnormal<br \/>\nglial biology, the gene dosage effect of other HSA21 genes, such as epigenetic modulators that<br \/>\ncan contribute further to the abnormal development of oligodendrocytes, deficient myelination,<br \/>\nand altered communication between neuronal and glia cells.<\/p>\n<p>&#8211; Using DS-derived COs, enriched with oligodendrocytes we demonstrated an abnormal<br \/>\ncommitment and differentiation of oligodendrocyte lineage in trisomy and detected a deficient<br \/>\nexpression of myelin-related markers, such as myelin basic protein (MBP) and Brain Enriched<br \/>\nMyelin Associated Protein 1 (BCAS1), observed in actively myelinating oligodendrocytes in<br \/>\ntrisomic CO (<b>Fig.1<\/b>). Using this system, we are now able to fully characterize the effect of<br \/>\ntrisomy 21 on oligodendrocyte development across different stages of differentiation.<br \/>\nFurthermore, we are currently investigating the correlation between alterations in the epigenetic<br \/>\nlandscape and changes in gene expression leading to the abnormal temporal development of<br \/>\noligodendrocytes and neurons in COs using a newly developed Multiome ATAC + Gene<br \/>\nExpression platform (10x Genomics).<\/p>\n<p>In our previous single-cell RNA sequencing (scRNA-seq) analysis of euploid and<br \/>\ntrisomic COs, we identified a cluster of X-linked genes containing brain-expressed X-<br \/>\nlinked\/transcription elongation factor A (SII) (BEX\/TCEAL) family members, that is<br \/>\ndownregulated in trisomy. At the same time, trisomic cells showed a significantly increased<br \/>\nexpression of non-coding X-inactive specific transcript (XIST), responsible for the X-<br \/>\nchromosome inactivation. Noticeably, other recent studies, including those performed in DS cell<br \/>\nlines and human postmortem brains, showed downregulated expression of different genes in<br \/>\nthis cluster accompanied by enhanced XIST levels. However, the aberrant expression of these<br \/>\ntranscription factors and their potential connection to brain pathology in trisomy has been<br \/>\nneglected. Herein, our current studies are aimed to causatively link the dysregulation of XIST<br \/>\nand X chromosome-linked genes to abnormal brain development and AD pathology in DS.<br \/>\n<br \/>Our next goals:<\/p>\n<p style=\"margin-left: 8px;\">&#8211; To understand the role of HMGN1, the HSA21 gene, a nucleosome remodeler in the<br \/>\nspecific transcriptomic changes in gene expression leading to the abnormal lineage<br \/>\ndevelopment and asynchronous maturation of neuronal and glia cells.<\/p>\n<p style=\"text-align: center;\" class=\"try-again\" onclick=\"document.body.classList.remove('active')\">Back<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p><!-- ___________________Popup 3___________________ --><\/p>\n<div class=\"popup2\">\n<div class=\"popup-inside2\">\n<div class=\"backgrounds2\">\n<div class=\"background2\"><\/div>\n<div class=\"background2 background22\"><\/div>\n<div class=\"background2 background32\"><\/div>\n<div class=\"background2 background42\"><\/div>\n<div class=\"background2 background52\"><\/div>\n<div class=\"background2 background62\"><\/div>\n<\/div>\n<\/div>\n<div class=\"content2\">\n<div class=\"content2-wrapper\">\n<p style=\"text-align: right; margin-top: -50px; margin-right: -50px;\" onclick=\"document.body.classList.remove('active2')\"><i class=\"close fa fa-times fa-4x\"><\/i><\/p>\n<div class=\"p-content\">\n<h2 class=\"pop\"><a class=\"news\" href=\"#\">Single cell transcriptomic profiling of cortical neurons underlying their regional diversity and selective vulnerability-collaborative studies with Dr. Luebke and Dr. Medalla.<\/h2>\n<p><\/a><\/p>\n<p>Through the collaboration with Drs. Luebke and Medalla we have established the extremely novel and technically-challenging Patch-seq approach to correlate functional properties of different populations of neurons in cerebral cortex with their molecular signature. Our lab leads the molecular part of the experiments, generating cDNA from individually isolated neurons and subsequent library preparation and sequencing (Fig. 1). One of the efforts is directed to obtain transcriptomic and functional profile of L3 cortical pyramidal cells in the LPFC, ACC and V1 and to assess the differential vulnerability of these neurons to age-related changes in monkeys. Another effort is dedicated to the assessment of the molecular signature that underlies the functional diversity of lamina-specific anterior cingulate cortex (ACC) cell types and ACC projection neurons directed to the amygdala, which are implicated in regulating emotional stress. <\/p>\n<p>In addition, we are currently in the process of analyzing single nuclei RNA-seq profiling of three regions of primate brain in order to elucidate spatial and laminar differences in gene expression within and between young and old animals.<\/p>\n<p><img src=\"\/anatneuro\/files\/2021\/10\/P2I1.png\" alt=\"\"\/><\/p>\n<p style=\"text-align: center;\" class=\"try-again\" onclick=\"document.body.classList.remove('active2')\"\">Back<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p><!-- __________________Popup 2___________________ --><\/p>\n<div class=\"popupC\">\n<div class=\"popup-insideC\">\n<div class=\"backgroundsC\">\n<div class=\"backgroundC\"><\/div>\n<div class=\"backgroundC background2C\"><\/div>\n<div class=\"backgroundC background3C\"><\/div>\n<div class=\"backgroundC background4C\"><\/div>\n<div class=\"backgroundC background5C\"><\/div>\n<div class=\"backgroundC background6C\"><\/div>\n<\/div>\n<\/div>\n<div class=\"contentC\">\n<div class=\"contentC-wrapper\">\n<p style=\"text-align: right; margin-top: -50px; margin-right: -50px;\" onclick=\"document.body.classList.remove('activeC')\"><i class=\"close fa fa-times fa-4x\"><\/i><\/p>\n<div class=\"p-content\">\n<h2 class=\"pop\"><a class=\"news\" href=\"#\">Understanding neuronal dysfunction in Down Syndrome using cortical organoids.<\/h2>\n<p><\/a><\/p>\n<p>Intellectual deficits and early onset of AD are the hallmarks<br \/>\nof the Down Syndrome brain pathology. We have previously<br \/>\ngenerated cortical organoids (COs) derived from isogenic<br \/>\niPS cell lines. We showed that our COs display global gene<br \/>\ndysregulation, abnormal neuronal development, increased<br \/>\ncell death, and AD-related depositions. Using single cell<br \/>\nRNA-seq studies we showed that among 16 different cell<br \/>\ntypes identified within the COs, excitatory neurons cluster 4<br \/>\n(ExN4) corresponding transcriptomically to layer IV cortical<br \/>\nneurons is most profoundly affected by trisomy. We also<br \/>\ndemonstrated diminished production of the deep and<br \/>\nsuperficial layer neurons in trisomic COs. However, we still<br \/>\ndon\u2019t know how these transcriptomic and cellular aberration<br \/>\ncontribute to neuronal disfunction in trisomy.<\/p>\n<p>Thus, we further expanded our studies to the functional<br \/>\nassessments using calcium imaging of GCaMP7s-<br \/>\nexpressing neurons and uncovered that neurons in trisomic<br \/>\nCOs had more synchronized oscillation pattern of calcium<br \/>\nsignals (Fig. 1A-E). Specifically, trisomic CO neurons<br \/>\nexhibited greater synchrony (Fig. 1B, C) and significantly<br \/>\nhigher correlation of lower frequency calcium transients (Fig.<br \/>\n1C). We also found that euploid neurons display consistent,<br \/>\nshorter Ca 2+ events, while trisomic neurons display less<br \/>\nconsistent, much longer Ca 2+ events, indicating defects in<br \/>\nactivity-dependent internal calcium dynamics that can affect<br \/>\npathological downstream cascades in DS (Fig. 1D, Di).<br \/>\nThese data are in line with previous work showing<br \/>\nhypersynchrony of adjacent brain regions in people with DS<br \/>\nand in the Ts65Dn mouse model of DS linked with deficits in<br \/>\nneuronal network maturation and neuronal processing. Our<br \/>\nability to recapitulate phenotypic, transcriptomic, and<br \/>\nfunctional dysregulation in neuronal populations in trisomy<br \/>\nallows us to further investigate the pathomechanisms<br \/>\nleading to abnormal connectivity in DS brain and resulting<br \/>\npotentially in intellectual deficits.<\/p>\n<p><img src=\"\/anatneuro\/files\/2023\/10\/P2I1.jpg\" alt=\"\"\/><\/p>\n<p>Our next goals:<br \/>\n        &#8211; To understand how the incorporation of euploid and trisomic microglia affects neuronal dysfunction in trisomy<br \/>\n        &#8211; To understand whether and how the protein and miRNA cargo of extracellular vesicles derived from diverse populations of brain cells contributes to the aberrant cellular phenotypes observed in DS.<br \/>\n        &#8211; To uncover different migration-related phenotypes that can promote altered neuronal properties in DS<br \/>\n        &#8211; To elucidate the common transcriptomic and functional features that are common to DS and DS-AD and perform the correlation between neuronal dysfunction and AD-related pathological depositions\n<\/p>\n<p>Mesenchymal stem cells (MSCs) have been shown to reverse or slow neurodegenerative,<br \/>\nage and injury-related pathology, suppress inflammation and facilitate tissue repair, plasticity<br \/>\nand remyelination in animal models. Our collaborators, Dr. Tara Moore and Dr. Maya Medalla<br \/>\nshowed that MSC-derived extracellular vesicles (EVs), the putative active product of MSCs,<br \/>\npromote recovery of motor function in monkeys and rodents after ischemic injury, facilitate<br \/>\nmicroglial shift towards restorative functions as well as promote myelin plasticity and<br \/>\nremyelination. While the animal models are indispensable for initial design and testing of<br \/>\ntherapeutics, human stem cell derived in vitro models provide a unique opportunity for target<br \/>\nvalidation prior to clinical testing.<\/p>\n<p>In our studies, we generated oligocortical spheroids (OLS) containing neurons, astrocytes, and<br \/>\noligodendrocytes to elucidate molecular mechanisms related to injury and recovery in human<br \/>\ncells. Furthermore, our OLS generated from embryonic stem cell line are engineered to express<br \/>\ntdTomato under the control of the endogenous PDGFR\u03b1 receptor. Using this system, we are<br \/>\nable to assess the pro-myelinating effect of EVs demonstrated in vivo and to decipher the<br \/>\ntherapeutic link between EVs and the myelin producing cells across the range of developmental<br \/>\nstages.<\/p>\n<p>In addition, using our OLS system we are capable to mimic the stroke conditions through the<br \/>\ninduction of oxygen-glucose deprivation (OGD) and interrogate the effect of potential<br \/>\ntherapeutics on brain cell viability, oxidative damage and myelin biology following ischemic<br \/>\nconditions.<\/p>\n<p><img src=\"\/anatneuro\/files\/2023\/10\/P2I2.jpg\" alt=\"\"\/><\/p>\n<p style=\"text-align: center;\" class=\"try-again\" onclick=\"document.body.classList.remove('activeC')\"\">Back<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p><!-- ___________________Lab Members___________________ --><\/p>\n<div class=\"blur\">\n<article class=\"content__inner\" style=\"margin-top: 50px;\">\n<h1 class=\"content__title\">Lab Members<\/h1>\n<h3 class=\"content__author\">As of 3\/7\/25<\/h3>\n<\/article>\n<div class=\"row\">\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2021\/10\/Ella-picture-scaled.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Ella Zeldich, PhD<\/h5>\n<p>Principal Investigator<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2025\/03\/Maya-Weidman-Research-Specialist-1.jpeg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Maya Weidman, MS<\/h5>\n<p>Research Specialist<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2021\/10\/headshot-Natalie-scaled.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Natalie Campbell<\/h5>\n<p>PhD Student<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2023\/10\/ElizabethKharitonovaPhD-scaled-e1696351354950.jpeg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Elizabeth Kharitonova<\/h5>\n<p>PhD Student<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2025\/03\/Samra_Beyene-MD.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Samra Beyene<\/h5>\n<p>MD Student<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2025\/03\/Marie-Shi-MDPhD-1.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Marie Shi<\/h5>\n<p>MD-PhD Student<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2023\/03\/clara-e1678136243786.png');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Clara Chung<\/h5>\n<p>Undergraduate Student<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2025\/03\/Samantha-Chung-Undergrad-1.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Samantha Chung<\/h5>\n<p>Undergraduate Student<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2025\/03\/Laura-Davies-Undergrad-2.png');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Laura Davies<\/h5>\n<p>Undergraduate Student<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2023\/10\/Grace-Field-scaled.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Grace Field<\/h5>\n<p>Undergraduate Student<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2025\/03\/Michelle-Kim.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Michelle Kim<\/h5>\n<p>Undergraduate Student<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2025\/03\/Jimmy-Zheng-Undergrad.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Jimmy Zheng<\/h5>\n<p>Undergraduate Student<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<\/div>\n<p><!-- ___________________Alumni___________________ --><\/p>\n<div class=\"blur\">\n<article class=\"content__inner\" style=\"margin-top: 50px;\">\n<h1 class=\"content__title\">Alumni<\/h1>\n<h3 class=\"content__author\">As of 3\/6\/23<\/h3>\n<\/article>\n<div class=\"row\">\n<div class=\"col-lg-4\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2023\/12\/sandeep.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Sandeep Rajkumar<\/h5>\n<p>Alumni<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-4\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2021\/10\/Ronni-Kurzion-picture-scaled.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Ronni Kurzion<\/h5>\n<p>Alumni<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-4\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2021\/10\/ProfilePic-Sanjeev.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Sanjeev Rampam<\/h5>\n<p>Alumni<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-4\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2021\/10\/Patel_Yesha_Headshot-copy.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Yesha Patel<\/h5>\n<p>Alumni<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-4\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2021\/10\/Cecila-Downs.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Cecila Downs<\/h5>\n<p>Alumni<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-4\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2023\/03\/sean.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Sean Farley<\/h5>\n<p>Alumni<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<\/div>\n<p><!-- ___________________Announcements___________________ --><\/p>\n<div class=\"blur\" style=\"display: none;\">\n<article class=\"content__inner\" style=\"margin-top: 50px;\">\n<h1 class=\"content__title\">Announcements<\/h1>\n<h3 class=\"content__author\"><\/h3>\n<\/article>\n<div class=\"row\" style=\"margin: auto; padding-top: 30px; padding-bottom: 30px;\">\n<p><!-- ___________________Announcements Block___________________ --><\/p>\n<div class=\"col-lg-12\" style=\"margin-top: 300px; margin-bottom: 300px; display: none;\">\n<div class=\"wrapper2\">\n<div class=\"popup4\">\n<div class=\"popup-inside4\"><\/div>\n<div class=\"content4\">\n<div class=\"content4-wrapper\">\n<div class=\"news-padding\">\n<article class=\"post\">\n<div class=\"newsscrollbar\">\n<div class=\"overflow\">\n<h1 class=\"news-headline\">Postdoctoral Position:<\/h1>\n<p class=\"news-headline\" style=\"text-align: left;\"><b>Job description:<\/b> We are looking for an energetic and highly motivated molecular and cellular biologist or stem cell biologist to join our team. Research in the laboratory aims to understand the molecular and epigenetic mechanisms mediating brain cell abnormalities in Down Syndrome and its comorbidity with Alzheimer\u2019s disease. Our studies are based on novel cutting-edge approaches utilizing human induced pluripotent stem cells (hiPSCs)-derived 3D in vitro models, including organoids and assembloids, CRISPR\/Cas9 genome editing, as well as single-cell transcriptomic and multiome profiling. We are also in the process of establishing a platform for the assessment of the electrophysiological properties of cortical organoids for translational research.<\/p>\n<p class=\"news-headline\" style=\"text-align: left;\">The candidate will join a multidisciplinary and highly collaborative research team that includes computational geneticists, neuroscientists, and electrophysiologists in the department and within BU School of Medicine. The ideal candidate will be able to lead a project, take a part in the development of research proposals, and mentoring of graduate and undergraduate students. Bioinformatics experience is a plus.<\/p>\n<p class=\"news-headline\" style=\"text-align: left;\">In addition, those interested would have plentiful opportunities to teach graduate courses in neuroscience and anatomy.<br \/>\nThe position is based on the recently-funded NIH grants and is available immediately.\n<\/p>\n<p class=\"news-date\">As of March 6th, 2023<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/article>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p><!-- ___________________News___________________ --><\/p>\n<div class=\"blur\">\n<article class=\"content__inner\" style=\"margin-top: 50px;\">\n<h1 class=\"content__title\">Zeldich Lab News<\/h1>\n<h3 class=\"content__author\"><\/h3>\n<\/article>\n<div class=\"row\" style=\"margin: auto; padding-top: 30px; padding-bottom: 30px;\">\n<p><!-- ___________________News Block___________________ --><\/p>\n<div class=\"col-lg-12\" style=\"margin-top: 300px; margin-bottom: 300px;\">\n<div class=\"wrapper2\">\n<div class=\"popup3\">\n<div class=\"popup-inside3\"><\/div>\n<div class=\"content3\">\n<div class=\"content3-wrapper\">\n<div class=\"news-padding\">\n<article class=\"post\">\n<div class=\"newsscrollbar\">\n<div class=\"overflow\">\n<p class=\"news-headline\">R01: Understanding neuronal dysfunction in Down Syndrome using assembloids and xenotransplanted cortical organoids<\/p>\n<p class=\"news-date\">October, 2024<\/p>\n<hr class=\"solid\">\n<p class=\"news-headline\">R21: The contribution of X-chromosome-linked genes to cellular phenotypes and AD-related pathology in Down Syndrome<\/p>\n<p class=\"news-date\">December, 2022<\/p>\n<hr class=\"solid\">\n<p class=\"news-headline\">R21:  Epigenetic determinants in oligodendrocyte maturation in Down Syndrome.<\/p>\n<p class=\"news-date\">July, 2022<\/p>\n<hr class=\"solid\">\n<p class=\"news-headline\">The Zeldich lab receives a new RO3 grant: Studying epigenetic dysregulation in Down Syndrome using fluorescent reporter cell lines.<\/p>\n<p class=\"news-date\">April 14, 2022<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/article>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p><!-- ___________________Apps___________________ --><\/p>\n<div class=\"blur\">\n<article class=\"content__inner\" style=\"margin-top: 50px;\">\n<h1 class=\"content__title\">Zeldich Lab Applications<\/h1>\n<h3 class=\"content__author\"><\/h3>\n<\/article>\n<div class=\"row\" style=\"margin: auto; padding-top: 30px; padding-bottom: 30px;\">\n<div class=\"col-lg-12\">\n<div class=\"button2\" style=\"margin: auto; margin-top: 20px; margin-bottom: 20px;\">\n<p><a class=\"box-shade\" href=\"https:\/\/www.dropbox.com\/sh\/3t56wwzmoxx6k11\/AACJe5LqiWsDqONQEWd-iGo2a?dl=0\" style=\"background-color: rgba(0,0,0,0);\"><\/a><br \/>\n<img style=\" width: 440px; height: 240px; object-fit: cover;\" src=\"\/anatneuro\/files\/2021\/10\/Background-for-panel-2.jpg\" alt=\"\"><br \/>\n<span style=\"color: #ffffff; position: absolute; z-index: 3; font-weight: normal; letter-spacing: 1px;\" class=\"button-text\"><a style=\"color: #ffffff; text-decoration: none;\" href=\"https:\/\/www.dropbox.com\/sh\/3t56wwzmoxx6k11\/AACJe5LqiWsDqONQEWd-iGo2a?dl=0\">ACEq Counting App<\/a><\/span><\/p>\n<div class=\"button-backgrounds2\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"r-body row\">\n<div class=\"heading\">\n<h1 style=\"color: #ffffff; padding-top: 10vh;\">Recent Publications<\/h1>\n<\/div>\n<div class=\"container22\" style=\"overflow: auto; height: 500px;\">\n<div class=\"list\">\n<div class=\"num\">\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\"> Zeldich E and Rajkumar S. Identity and Maturity of iPSC-Derived Oligodendrocytes in 2D and Organoid Systems. Cells. 2024 Apr 13;13(8):674. doi: 10.3390\/cells13080674<\/h3>\n<\/p><\/div>\n<div class=\"num\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36834891\/\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Campbell NB, Patel Y, Moore TL, Medalla M, Zeldich E.  Extracellular Vesicle Treatment Alleviates Neurodevelopmental and Neurodegenerative Pathology in Cortical Spheroid Model of Down Syndrome. Int J Mol Sci; 2023 Feb 9;24(4):3477. doi: 10.3390\/ijms24043477 (PMID:36834891)<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36463299\/\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Farley, SJ, Grishok A, Zeldich E.  Shaking up the silence: consequences of HMGN1 antagonizing PRC2 in the Down syndrome brain. Epigenetics Chromatin. 2022; 15(1):39. doi: 10.1186\/s13072-022-00471-6. (PMID:36463299)<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36161168\/\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Li Z, Klein JA, Rampam S, Kurzion R, Campbell NB, Patel Y, Haydar TF, Zeldich E.  Asynchronous excitatory neuron development in an isogenic cortical spheroid model of Down syndrome . Frontiers Neurosci., 2022, 7;16:932384. doi: 10.3389\/fnins.2022.932384 (PMID:36161168)<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/35058753\/\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Klein JA, Li Z, Rampam S, Cardini J, Ayoub A, Shaw, P, Rachubinski AL, Espinosa JM, Zeldich E, Haydar TF. Sonic Hedgehog Pathway Modulation Normalizes Expression of Olig2 in Rostrally Patterned NPCs with Trisomy 21. Frontiers Cellular Neurosci. 15:794675. doi:10.3389\/fncel.2021.794675. (PMID: 35058753)<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2021.10.19.465029v1\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Klein JA, Li Z, Rampam S, Cardini J, Ayoub A, Shaw P, Rachubinski L, Espinosa J, Zeldich E, Haydar T.  Sonic hedgehog pathway modulation normalized expression of olig2 in rostrally patterned NPCs with trisomy 21. BioRxiv. 2021; doi: https:\/\/doi.org\/10.1101\/2021.10.19.465029<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8431824\/ \"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Shelton SM, Soucy AR, Kurzion R, Zeldich E, Connor JH, Haydar TF. Forebrain neural precursor cells are differentially vulnerable to zika virus infection. eNeuro. 2021;8(5). doi:10.1523\/ENEURO.0108-21.2021<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8431824\/\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Shelton SM, Soucy AR, Kurzion R, Zeldich E, Connor JH, Haydar TF. Forebrain Neural Precursor Cells Are Differentially Vulnerable to Zika Virus Infection. eNeuro. 2021;8(5):ENEURO.0108-21.2021. Published 2021 Sep 9. doi:10.1523\/ENEURO.0108-21.2021<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32347987\/\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Chen C di, Rudy MA, Zeldich E, Abraham CR. A method to specifically activate the Klotho promoter by using zinc finger proteins constructed from modular building blocks and from naturally engineered Egr1 transcription factor backbone. FASEB Journal. 2020;34(6). doi:10.1096\/fj.202000171R<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/33158872\/\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Li Z, Tyler WA, Zeldich E, et al. Transcriptional priming as a conserved mechanism of lineage diversification in the developing mouse and human neocortex. Science Advances. 2020;6(45). doi:10.1126\/SCIADV.ABD2068<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31929539\/ \"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Chen CD, Li Y, Chen AK, Rudy MA, Nasse J S, Zeldich E, Polanco TJ, Abraham C R. Identification of the cleavage sites leading to the shed forms of human and mouse anti-aging and cognition-enhancing protein Klotho. PloS one, 2020;15(1), e0226382. https:\/\/doi.org\/10.1371\/journal.pone.0226382<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/30776010\/ \"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Chen C di, Zeldich E, Khodr C, et al. Small Molecule Amyloid-\u03b2 Protein Precursor Processing Modulators Lower Amyloid-\u03b2 Peptide Levels via cKit Signaling. Journal of Alzheimer\u2019s Disease. 2019;67(3). doi:10.3233\/JAD-180923<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/29352444\/ \"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Chen C di, Zeldich E, Li Y, Yuste A, Abraham CR. Activation of the Anti-Aging and Cognition-Enhancing Gene Klotho by CRISPR-dCas9 Transcriptional Effector Complex. Journal of Molecular Neuroscience. 2018;64(2). doi:10.1007\/s12031-017-1011-0<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/29516269\/ \"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Ikezu T, Chen C, DeLeo AM, et al. Tau Phosphorylation is Impacted by Rare AKAP9 Mutations Associated with Alzheimer Disease in African Americans. Journal of Neuroimmune Pharmacology. 2018;13(2). doi:10.1007\/s11481-018-9781-x<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27125744\/ \"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Abraham, C. R., Mullen, P. C., Tucker-Zhou, T., Chen, C. D., &#038; Zeldich, E. (2016). Klotho Is a Neuroprotective and Cognition-Enhancing Protein. Vitamins and hormones, 101, 215\u2013238. https:\/\/doi.org\/10.1016\/bs.vh.2016.02.004<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23365232\/ \"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Zeldich E, Chen C di, Avila R, Medicetty S, Abraham CR. The Anti-Aging Protein Klotho Enhances Remyelination Following Cuprizone-Induced Demyelination. Journal of Molecular Neuroscience. 2015;57(2). doi:10.1007\/s12031-015-0598-2<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/25037225\/ \"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Zeldich E, Chen C di, Colvin TA, et al. The neuroprotective effect of Klotho is mediated via regulation of members of the redox system. Journal of Biological Chemistry. 2014;289(35). doi:10.1074\/jbc.M114.567321<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/25043464\/ \"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Jun G, Asai H, Zeldich E, et al. PLXNA4 is associated with Alzheimer disease and modulates tau phosphorylation. Annals of Neurology. 2014;76(3). doi:10.1002\/ana.24219<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23365232\/ \"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Chen C di, Sloane JA, Li H, et al. The antiaging protein klotho enhances oligodendrocyte maturation and myelination of the CNS. Journal of Neuroscience. 2013;33(5). doi:10.1523\/JNEUROSCI.2080-12.2013<\/h3>\n<p>    <\/a><\/div>\n<\/p><\/div>\n<\/div>\n<\/div>\n<div class=\"row\">\n<div class=\"col-md-6\">\n<div id=\"tv\">\n<div class=\"universal-padding\">\n<h2 class=\"tv-title\">Contact Us<\/h2>\n<p class=\"contacts\">Zeldich Lab<\/p>\n<p class=\"contacts\">780 Harrison Ave., R-903<\/p>\n<p class=\"contacts\">Boston Massachusetts, 02118<\/p>\n<p class=\"contacts\">Phone: 617-358-0811<\/p>\n<p class=\"contacts\">Email: ezeldich@bu.edu<\/p>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"col-md-6\">\n<img loading=\"lazy\" src=\"\/anatneuro\/files\/2023\/10\/Image-for-the-lab.jpeg\" width=\"400\" height=\"175\" class=\"info-img\">\n<\/div>\n<\/div>\n<\/div>\n<p><!-- ___________________Page CSS___________________ --><\/p>\n<style>\n<p>\/* ----------------News Section---------------- *\/\n.newsscrollbar::-webkit-scrollbar {\n\tbackground-color: #f3f4f5;\n\twidth: 16px;\n}<\/p>\n<p>.newsscrollbar::-webkit-scrollbar-track 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#262262;\n\tpadding-bottom: 5px;\n}<\/p>\n<p>.news-headline {\n  margin: 0;\n  font-size: 16px;\n  text-align: center;\n  color: #262262;\n}<\/p>\n<p>.headline2 {\n  margin: 0;\n  font-size: 16px;\n  text-align: center;\n  color: #262262;\n}<\/p>\n<p>.news-date {\n  font-weight: 700;\n  text-align: center;\n  color: #9f9f9f;\n}<\/p>\n<p>.news-border {\n   border-bottom: 2px solid #e7078b;\n}<\/p>\n<p>hr.solid {\n  border-top: 1px solid #e4e4e5;\n  margin-bottom: 15px;\n}<\/p>\n<p>h3.news-title1 {\n\tfont-weight: 800;\n\tfont-size: 3em;\n\ttext-align: center;\n\tline-height: .9em;\n}<\/p>\n<p>\/* ----------------Blocks---------------- *\/<\/p>\n<p>.popup3{\n    opacity: 1;\n    visibility: visible;\n    height: 600px;\n    width: 500px;\n    flex-shrink: 0;\n    border-radius: 3px;\n    position: absolute;\n    display: flex;\n    align-items: center;\n    z-index: 2;\n}<\/p>\n<p>.popup-inside3{\n    opacity: 1;\n    position: absolute;\n    left: 0;\n    top: 0;\n    height: 100%;\n    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100%;\n    width: 100%;\n    overflow: hidden;\n    border-radius: 0;\n    box-shadow: -50px 0 100px -50px #808080, 50px 0 100px -50px #808080;\n    transition:\n            box-shadow 0.5s ease 0.7s,\n            border-radius 0.35s ease 0.7s;\n}<\/p>\n<p>.content4{\n    --offset: 0;\n    position: absolute;\n    left: var(--offset);\n    right: var(--offset);\n    bottom: var(--offset);\n    top: var(--offset);\n    display: flex;\n    align-items: center;\n    justify-content: center;\n    opacity: 1;\n    transform: none;\n    z-index: 10;<\/p>\n<p>}<\/p>\n<p>.content4-wrapper{\n    text-align: center;\n    padding-left: 20px;\n    padding-right: 20px;\n}<\/p>\n<p>.wrapper2{\n    left: 0;\n    top: 0;\n    width: 100%;\n    height: 100%;\n    display: flex;\n    align-items: center;\n    justify-content: center;\n    z-index: 5;\n}<\/p>\n<p>html, body {\n    width: 100%;\n    height: 100%;<\/p>\n<p>}<\/p>\n<p>.main1 {\n  margin: auto;\n  position: absolute;\n  padding: 10px 5px;\n  background: rgba(255, 255, 255, 1);\n  border-radius: 10px;\n  overflow: hidden;\n  z-index: 2;\n  height: 50%;\n  width: 95%;\n  filter: blur(3px);\n}<\/p>\n<p>.blur {\n}<\/p>\n<p>body.active .blur {\nfilter: blur(2px);\n}<\/p>\n<p>body.active2 .blur {\nfilter: blur(2px);\nz-index: 3;\n}<\/p>\n<p>body.activeC .blur {\nfilter: blur(2px);\nz-index: 3;\n}<\/p>\n<p>.universal-pad {\nmargin: 2vw;\t\n}<\/p>\n<p>.close:hover {\n  color: #00aeef;\n}<\/p>\n<p>.fade {\n   color: #ffffff;\n   left: 0;\n   right: 0;\n   top: 0;\n   margin-bottom: 50px;\n   background: linear-gradient(-45deg, #7d7d7d, #949494, #b0b0b0, #c7c7c7);\n\tbackground-size: 400% 400%;\n\tanimation: gradient 15s ease infinite;\n}<\/p>\n<p>@keyframes gradient {\n\t0% {\n\t\tbackground-position: 0% 50%;\n\t}\n\t50% {\n\t\tbackground-position: 100% 50%;\n\t}\n\t100% {\n\t\tbackground-position: 0% 50%;\n\t}\n}<\/p>\n<p>p.title {\n  color: #ffffff;\n  font-size: 35px;\n  line-height: 40px;\n  text-align: left;\n}<\/p>\n<p>.content__inner {\n  margin: 0 auto;\n  max-width: 700px;\n}\n.content__inner > * + * {\n  margin-top: 1.5rem;\n}\n.content__inner > blockquote {\n  margin: 3rem 0;\n}<\/p>\n<p>.content__title {\n\tfont-family: 'Open Sans', sans-serif;\n  font-size: 3rem;\n  line-height: 1.25;\n  letter-spacing: -0.125rem;\n  text-align: center;<\/p>\n<p>}\n@media (min-width: 600px) {\n  .content__title {\n    font-size: 4rem;\n  }\n}<\/p>\n<p>.content__author {\n  display: flex;\n  align-items: center;\n  justify-content: space-between;\n  margin-bottom: 4rem;\n  width: 100%;\n\tfont-family: 'Open Sans', sans-serif;\n  font-size: 1.5rem;\n  letter-spacing: -0.125rem;\n  text-align: center;\n}\n.content__author:before, .content__author:after {\n  content: \"\";\n  flex: 1;\n  height: 2px;\n  background-color: #262262;\n}\n.content__author:before {\n  margin-right: 1rem;\n}\n.content__author:after {\n  margin-left: 1rem;\n}<\/p>\n<p>\/* ----------------Info Image---------------- *\/<\/p>\n<p>.info-img {\n  margin: 3vw;\n  box-shadow: 0 4px 8px 0 rgba(0, 0, 0, 0.2), 0 6px 20px 0 rgba(0, 0, 0, 0.19);\n  border-radius: 8px;\n}<\/p>\n<p>\/* ----------------People---------------- *\/<\/p>\n<p>.property-card h5\n{\n  margin:0px;\n  font-size:1.4em;\n  font-weight:700;\n}<\/p>\n<p>.property-card p\n{\n  font-size:12px;\n  text-align: center;\n}<\/p>\n<p>.center\n{\nwidth: 50%;\n}<\/p>\n<p>\/* End Non-Essential  *\/<\/p>\n<p>.property-card\n{\n  margin: auto;\n  margin-bottom: 20px;\n  height:375px;\n  width:280px;\n  display:-webkit-box;\n  display:-ms-flexbox;\n  display:flex;\n  -webkit-box-orient:vertical;\n  -webkit-box-direction:normal;\n  -ms-flex-direction:column;\n  flex-direction:column;\n  position:relative;<\/p>\n<p>  border-radius:16px;\n  overflow:hidden;\n  -webkit-box-shadow:  15px 15px 27px #e1e1e3, -15px -15px 27px #ffffff;\n  box-shadow:  15px 15px 27px #e1e1e3, -15px -15px 27px #ffffff;\n}\n\/* ^-- The margin bottom is necessary for the drop shadow otherwise it gets clipped in certain cases. *\/<\/p>\n<p>\/* Top Half of card, image. *\/<\/p>\n<p>.property-image\n{\n  height:313px;\n  width:280px;\n  padding:1em 2em;\n  position:absolute;\n  top:0px;<\/p>\n<p>  background-size:cover;\n  background-repeat:no-repeat;\n}<\/p>\n<p>\/* Bottom Card Section *\/<\/p>\n<p>.property-description\n{\n  background-color: #FAFAFC;\n  height:62px;\n  width:280px;\n  position:absolute;\n  bottom:0em;<\/p>\n<p>  padding: 0.5em 1em;\n  text-align:center;\n}<\/p>\n<p>\/* Social Icons *\/<\/p>\n<p>.property-social-icons\n{\n  width:1em;\n  height:1em;\n  background-color:black;\n  position:absolute;\n  bottom:1em;\n  left:1em;\n  -webkit-transition:all 0.4s cubic-bezier(0.645, 0.045, 0.355, 1);\n  -o-transition:all 0.4s cubic-bezier(0.645, 0.045, 0.355, 1);\n  transition:all 0.4s cubic-bezier(0.645, 0.045, 0.355, 1);\n}<\/p>\n<p>\/* Property Cards Hover States *\/<\/p>\n<p>.property-card:hover .property-social-icons\n{\n  background-color:white;\n}<\/p>\n<p>.property-card:hover .property-social-icons:hover\n{\n  background-color:blue;\n  cursor:pointer;\n}<\/p>\n<p>\/* ----------------Border---------------- *\/<\/p>\n<p>#tv {\n  position: relative;\n  width: 350px;\n  height: 350px;\n  background: white;\n  border-radius: 0% 0% 0% 0% \/ 0% 0% 0% 0% ;\n  box-shadow: 20px 20px rgba(0,0,0,.15);\n  transition: all .4s ease;\n  margin: auto;\n  margin-top: 5vh;\n  margin-bottom: 5vh;\n  padding: 25px;\n}\n#tv:hover {\n  border-radius: 0% 0% 50% 50% \/ 0% 0% 5% 5% ;\n  box-shadow: 10px 10px rgba(0,0,0,.25);\n}<\/p>\n<p>.contacts {\n  margin: 10px;\n}<\/p>\n<p>.tv-title {\n  position: relative;\n}<\/p>\n<p>.tv-title::after {\n  content: \"\";\n  position: absolute;\n  height: 4px;\n  left: 0;\n  bottom: -10px;\n  width: 20%;\n  background: #262262\n}<\/p>\n<p>\/* ----------------Links---------------- *\/<\/p>\n<p>.r-body {\n  margin: 0;\n background: linear-gradient(-45deg, #7d7d7d, #949494, #b0b0b0, #c7c7c7);\n\tbackground-size: 400% 400%;\n\tanimation: gradient 15s ease infinite;\n}\n.heading {\n  color: #ffffff;\n  text-align: center;\nmargin-top: 10vw;\n}\n.heading h1 {\n  margin-bottom: 3rem;\n  font-weight: 300;\n  position: relative;\n}\n.heading h1:after {\n  content: '';\n  height: 2px;\n  width: 0rem;\n  position: absolute;\n  left: 50%;\n  bottom: -1rem;\n  transform: translateX(-50%);\n  background-color: #fff;\n}\n.container22 {\n  background-color: #fff;\n  padding: 2rem;\n  margin-right: 10vw;\n  margin-left: 10vw;\n  margin-bottom: 10vw;\n  box-shadow: 4px 4px 8px rgba(0,0,0,0.1);\n}\n.container22 .nav {\n  background-color: #fafafa;\n  display: flex;\n  align-items: center;\n  justify-content: center;\n  padding: 1rem;\n  margin: 0 -3rem 2rem;\n  box-shadow: 2px 2px 4px rgba(0,0,0,0.1);\n  position: relative;\n}\n.container22 .nav:before,\n.container22 .nav:after {\n  content: '';\n  height: 0;\n  width: 0;\n  position: absolute;\n  top: 0;\n  border: 0.75rem solid transparent;\n  border-bottom: 0.75rem solid #f2f0f0;\n  transform-origin: center;\n  box-shadow: 2px 2px 4px rgba(0,0,0,0.1);\n  z-index: -1;\n}\n.container22 .nav:before {\n  left: 0;\n  transform: translateY(-0.45rem) rotate(135deg) translateX(-0.4rem);\n}\n.container22 .nav:after {\n  right: 0;\n  transform: translateY(-0.45rem) rotate(-135deg) translateX(0.4rem);\n}\n.container22 .nav a {\n  display: inline-block;\n  margin: 0 3rem;\n  font-size: 2rem;\n  color: #2980b9;\n  opacity: 0.7;\n  transition: 0.25s;\n}\n.container22 .nav a:hover {\n  opacity: 1;\n}\n.container22 .list .num {\n  padding: 0.5rem 2rem;\n  display: flex;\n  align-items: center;\n  justify-content: flex-start;\n  transition: 0.25s;\n}\n.container22 .list .num:nth-child(0):before {\n  content: '0';\n  font-size: 4rem;\n  font-weight: normal;\n  color: #000;\n  width: 2rem;\n  opacity: 0.05;\n  transition: 0.25s;\n}\n.container22 .list .num:nth-child(1):before {\n  content: '';\n  font-size: 4rem;\n  font-weight: 100;\n  color: #000;\n  width: 2rem;\n  opacity: 0.05;\n  transition: 0.25s;\n}\n.container22 .list .num:nth-child(2):before {\n  content: '';\n  font-size: 4rem;\n  font-weight: normal;\n  color: #000;\n  width: 2rem;\n  opacity: 0.05;\n  transition: 0.25s;\n}\n.container22 .list .num:nth-child(3):before {\n  content: '';\n  font-size: 4rem;\n  font-weight: normal;\n  color: #000;\n  width: 2rem;\n  opacity: 0.05;\n  transition: 0.25s;\n}\n.container22 .list .num:nth-child(4):before {\n  content: '';\n  font-size: 4rem;\n  font-weight: normal;\n  color: #000;\n  width: 2rem;\n  opacity: 0.05;\n  transition: 0.25s;\n}\n.container22 .list .num:nth-child(5):before {\n  content: '';\n  font-size: 4rem;\n  font-weight: normal;\n  color: #000;\n  width: 2rem;\n  opacity: 0.05;\n  transition: 0.25s;\n}\n.container22 .list .num:nth-child(6):before {\n  content: '';\n  font-size: 4rem;\n  font-weight: normal;\n  color: #000;\n  width: 2rem;\n  opacity: 0.05;\n  transition: 0.25s;\n}\n.container22 .list .num:nth-child(7):before {\n  content: '';\n  font-size: 4rem;\n  font-weight: normal;\n  color: #000;\n  width: 2rem;\n  opacity: 0.05;\n  transition: 0.25s;\n}\n.container22 .list .num:nth-child(8):before {\n  content: '';\n  font-size: 4rem;\n  font-weight: normal;\n  color: #000;\n  width: 2rem;\n  opacity: 0.05;\n  transition: 0.25s;\n}\n.container22 .list .num:nth-child(9):before {\n  content: '';\n  font-size: 4rem;\n  font-weight: normal;\n  color: #000;\n  width: 2rem;\n  opacity: 0.05;\n  transition: 0.25s;\n}\n.container22 .list .num:nth-child(10):before {\n  content: '';\n  font-size: 4rem;\n  font-weight: normal;\n  color: #000;\n  width: 2rem;\n  opacity: 0.05;\n  transition: 0.25s;\n}\n.container22 .list .num:nth-child(11):before {\n  content: '';\n  font-size: 4rem;\n  font-weight: normal;\n  color: #000;\n  width: 2rem;\n  opacity: 0.05;\n  transition: 0.25s;\n}\n.container22 .list .num:nth-child(12):before {\n  content: '';\n  font-size: 4rem;\n  font-weight: normal;\n  color: #000;\n  width: 2rem;\n  opacity: 0.05;\n  transition: 0.25s;\n}\n.container22 .list .num:nth-child(13):before {\n  content: '';\n  font-size: 4rem;\n  font-weight: normal;\n  color: #000;\n  width: 2rem;\n  opacity: 0.05;\n  transition: 0.25s;\n}\n.container22 .list .num:nth-child(14):before {\n  content: '';\n  font-size: 4rem;\n  font-weight: normal;\n  color: #000;\n  width: 2rem;\n  opacity: 0.05;\n  transition: 0.25s;\n}\n.container22 .list .num:nth-child(15):before {\n  content: '';\n  font-size: 4rem;\n  font-weight: normal;\n  color: #000;\n  width: 2rem;\n  opacity: 0.05;\n  transition: 0.25s;\n}\n.container22 .list .num:nth-child(16):before {\n  content: '';\n  font-size: 4rem;\n  font-weight: normal;\n  color: #000;\n  width: 2rem;\n  opacity: 0.05;\n  transition: 0.25s;\n}<\/p>\n<p>.container22 .list .num h3 {\n  position: relative;\n  left: -1.5rem;\n  color: #3d3d3d;\n  font-size: 13px;\n  transition: 0.25s;\n}\n.container22 .list .num:hover {\n  background-color: #fafafa;\n  cursor: pointer;\n}\n.container22 .list .num:hover:before {\n  opacity: 0.2;\n}\n.container22 .list .num:hover h3 {\n  left: 1rem;\n}<\/p>\n<p>.list a {\n  text-decoration: none;\n}<\/p>\n<p>\/* ----------------Table---------------- *\/<\/p>\n<p>.table-wrapper{\n    margin: auto;\n    width: 350px;\n    box-shadow: 0px 35px 50px rgba( 0, 0, 0, 0.2 );\n}<\/p>\n<p>.table-wrapper2{\n    margin: auto;\n    width: 85%;\n    box-shadow: 0px 35px 50px rgba( 0, 0, 0, 0.2 );\n}<\/p>\n<p>.fl-table {\n    border-radius: 5px;\n    font-size: 14px;\n    font-weight: normal;\n    border: none;\n    border-collapse: collapse;\n    width: 100%;\n    max-width: 100%;\n    white-space: normal;\n    background-color: white;\n}<\/p>\n<p>.fl-table td, .fl-table th {\n    text-align: center;\n    padding: 8px;\n}<\/p>\n<p>.fl-table td {\n    border-right: 1px solid #f8f8f8;\n    font-size: 14px;\n}<\/p>\n<p>.fl-table thead th {\n    color: #ffffff;\n    background: #00aeef;\n    font-size: 20px;\n}<\/p>\n<p>.fl-table thead th:nth-child(odd) {\n    color: #ffffff;\n    background: #1e326b;\n}<\/p>\n<p>.fl-table tr:nth-child(even) {\n    background: #f0f0f0;\n}<\/p>\n<p>\/* ----------------Popup 1---------------- *\/<\/p>\n<p>.wrapper1{\n    position: fixed;\n    left: 0;\n    top: 0;\n    width: 100%;\n    height: 100%;\n    display: flex;\n    align-items: center;\n    justify-content: center;\n     z-index: -1;<\/p>\n<p>}<\/p>\n<p>body.active .wrapper1{\n    z-index: 10;\n}<\/p>\n<p>.wrapper1 p{\n    padding: 2px;\n}<\/p>\n<p>.wrapper1 ul {\n  list-style-type: circle;\n}<\/p>\n<p>.wrapper1 p:last-of-type{\n    padding-bottom: 0;\n}<\/p>\n<p>.popup{\n    opacity: 0;\n    visibility: hidden;\n    height: 90vh;\n    width: 80vw;\n    flex-shrink: 0;\n    border-radius: 3px;\n    position: absolute;\n    z-index: 3;\n    display: flex;\n    align-items: center;\n    transition: all 0.2s ease\n    overflow: scroll;\n}<\/p>\n<p>.p-content {\n   overflow: auto;\n   height: 75vh; \n   width: 65vw;\"\n}<\/p>\n<p>.popup-inside{\n    position: absolute;\n    left: 0;\n    top: 0;\n    height: 100%;\n    width: 100%;\n    border-radius: 50%;\n    box-shadow: 0 0 0 black;\n    transition:\n            box-shadow 0.5s ease 0.7s,\n            border-radius 0.35s ease 0.7s;\n}<\/p>\n<p>.content1-wrapper{\n    text-align: center;\n    padding-left: 20px;\n    padding-right: 20px;\n}<\/p>\n<p>.backgrounds{\n    position: absolute;\n    left: 0;\n    top: 0;\n    height: 100%;\n    width: 100%;\n    overflow: hidden;\n}<\/p>\n<p>.background{\n    --offset: 0;\n    position: absolute;\n    left: var(--offset);\n    right: var(--offset);\n    bottom: var(--offset);\n    top: var(--offset);\n    background: linear-gradient(to right, #00aeef, #ec008c);\n    transform: scale(0);\n    transition: all 0.5s ease 0s;\n    border-radius: 50%;\n}<\/p>\n<p>.background2{\n    --offset: 10%;\n    position: absolute;\n    left: var(--offset);\n    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scale(8);\n}<\/p>\n<\/style>\n","protected":false},"excerpt":{"rendered":"<p>Laboratory of Molecular Signaling in Neurological Disorders (Zeldich Lab) Laboratory of Molecular Signaling in Neurological Disorders (Zeldich Lab) Our Research Our\u00a0lab\u00a0is focusing on studying the cellular and molecular machinery mediating the connection between Down Syndrome and Alzheimer\u2019s disease.\u00a0 We are utilizing novel and powerful 3D cellular models (organoids and assembloids) derived from human induced pluripotent [&hellip;]<\/p>\n","protected":false},"author":18550,"featured_media":0,"parent":0,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"page-templates\/no-sidebars.php","meta":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/anatneuro\/wp-json\/wp\/v2\/pages\/9877"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/anatneuro\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/anatneuro\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/anatneuro\/wp-json\/wp\/v2\/users\/18550"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/anatneuro\/wp-json\/wp\/v2\/comments?post=9877"}],"version-history":[{"count":51,"href":"https:\/\/www.bumc.bu.edu\/anatneuro\/wp-json\/wp\/v2\/pages\/9877\/revisions"}],"predecessor-version":[{"id":12669,"href":"https:\/\/www.bumc.bu.edu\/anatneuro\/wp-json\/wp\/v2\/pages\/9877\/revisions\/12669"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/anatneuro\/wp-json\/wp\/v2\/media?parent=9877"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}