{"id":10106,"date":"2021-11-05T13:25:01","date_gmt":"2021-11-05T17:25:01","guid":{"rendered":"https:\/\/www.bumc.bu.edu\/anatneuro\/?page_id=10106"},"modified":"2025-05-06T11:55:46","modified_gmt":"2025-05-06T15:55:46","slug":"roussarie-lab","status":"publish","type":"page","link":"https:\/\/www.bumc.bu.edu\/anatneuro\/roussarie-lab\/","title":{"rendered":"Roussarie Lab"},"content":{"rendered":"<\/div>\n<p><!-- ___________________Navbar___________________ --><\/p>\n<div id=\"navvy\"><\/div>\n<p><script type=\"text\/javascript\" src=\"\/anatneuro\/files\/2025\/05\/nav-5-2025.js\"><\/script><\/p>\n<p><!-- ___________________Banner___________________ --><\/p>\n<div class=\"mobile-title mobile-banner\" style=\"background-color: rgba(0,0,0,0);\">\n<p class=\"mobile-header\">Roussarie Lab<\/p>\n<\/div>\n<style>.mobile-banner{background-image: url(\"\/anatneuro\/files\/2021\/11\/banner-scaled.jpg\"); background-size: cover;}<\/style>\n<div class=\"blur\">\n<div class=\"rossariou-parallax\">\n<div class=\"true-nav nav-border\"><\/div>\n<div class=\"title-padding\">\n<p style=\"padding-top: 0px;\" class=\"title-header\">Roussarie Lab<\/p>\n<\/div>\n<\/div>\n<p><!-- ___________________Lab Details___________________ --><\/p>\n<div class=\"row\">\n<div class=\"col-md-7\">\n<div class=\"universal-pad\" style=\"margin-left: 2vw;\">\n<h2>Our Research:<\/h2>\n<p><b>Selective neuronal vulnerability: leveraging this underexplored property of Alzheimer\u2019s, to molecularly dissect the disease<\/b><\/p>\n<p>Like almost all neurodegenerative diseases, Alzheimer\u2019s disease in its earliest stages only affects very specific sets of neurons. In the majority of patients, neurons from the layer II of entorhinal cortex (ECII) are the first ones to accumulate pathological lesions and die. These neurons are crucial for assembling higher-order sensory information into new memories.<\/p>\n<p>The vulnerability of ECII neurons is very poorly understood. We think that understanding what differentiates vulnerable neurons from more resistant neurons might open new therapeutic avenues, a necessity for the Alzheimer\u2019s field. Vulnerability is a complex feature and likely results from a number of genes enriched\/impoverished in ECII neurons, in addition to the particular neuronal and glial environment surrounding these neurons.<\/p>\n<p>Using cell-type specific profiling techniques, and systems-level functional genomics, we demonstrated that ECII neurons possessed intrinsic features that could make them more vulnerable (Roussarie, Yao et al. Neuron, 2020). Further searching for hub genes driving pathological processes in vulnerable neurons, we identified a proto-oncogene, DEK, which regulates both tau accumulation and neuronal excitability (Rodriguez-Rodriguez et al., bioRxiv, 2022). We now want to continue exploring vulnerability to uncover the earliest events leading to neurodegeneration.<\/p>\n<p>Some of our major projects include:<\/p>\n<div style=\"text-align: center;\">\n<p style=\"margin-left: 50px; margin-right: 50px;\">\n        &#8211; Map cellular diversity within the EC across species<br \/>\n        &#8211; Develop an <em>in vitro<\/em> model for vulnerable neurons<br \/>\n        &#8211; Identify more drivers of neuronal vulnerability (which could become therapeutic targets) to gain insight into the different processes that regulate early stages of AD<\/p>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"col-md-5\">\n<div class=\"row\">\n<div class=\"col-md-6\">\n<img src=\"\/anatneuro\/files\/2022\/06\/EC_rainbow.jpg\" style=\"width: 90%; height: 90%;\" class=\"info-img\"><br \/>\n<img src=\"\/anatneuro\/files\/2021\/11\/image0.jpg\" style=\"width: 90%; height: 90%;\" class=\"info-img\"><br \/>\n<img src=\"\/anatneuro\/files\/2021\/11\/neuron_cover.jpg\" style=\"width: 90%; height: 90%;\" class=\"info-img\">\n<\/div>\n<div class=\"col-md-6\">\n<img src=\"\/anatneuro\/files\/2021\/11\/neuron.jpg\" style=\"width: 90%; height: 90%;\" class=\"info-img\"><br \/>\n<img src=\"\/anatneuro\/files\/2022\/08\/EC_rainbow.jpg\" style=\"width: 90%; height: 90%;\" class=\"info-img\">\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p><!-- ___________________Buttons___________________ --><\/p>\n<div class=\"row fade\" style=\"margin: auto; padding-top: 30px; padding-bottom: 30px;\">\n<div class=\"col-lg-4\">\n<div class=\"button2\" style=\"margin: auto; margin-top: 20px; margin-bottom: 20px;\" onclick=\"document.body.classList.add('active')\">\n<p><a class=\"box-shade\" style=\"background-color: rgba(0,0,0,0);\"><\/a><br \/>\n<img style=\" width: 440px; height: 240px; object-fit: cover;\" src=\"\/anatneuro\/files\/2021\/11\/image1.jpg\" alt=\"\"><br \/>\n<span style=\"z-index: 3; color: #ffffff; font-weight: normal; position: absolute;\" class=\"button-text\">Map cellular diversity<br \/> within the EC across species<\/span><\/p>\n<div class=\"button-backgrounds\"><\/div>\n<\/div>\n<\/div>\n<div class=\"col-lg-4\">\n<div class=\"button2\" style=\"margin: auto; margin-top: 20px; margin-bottom: 20px;\" onclick=\"document.body.classList.add('activeC')\">\n<p><a class=\"box-shade\" style=\"background-color: rgba(0,0,0,0);\"><\/a><br \/>\n<img style=\" width: 440px; height: 240px; object-fit: cover;\" src=\"\/anatneuro\/files\/2021\/11\/image2.jpg\" alt=\"\"><br \/>\n<span style=\"z-index: 3; color: #ffffff; font-weight: normal; position: absolute;\" class=\"button-text\">Develop an in vitro model for vulnerable neurons<\/span><\/p>\n<div class=\"button-backgrounds2\"><\/div>\n<\/div>\n<\/div>\n<div class=\"col-lg-4\">\n<div class=\"button2\" style=\"margin: auto; margin-top: 20px; margin-bottom: 20px;\" onclick=\"document.body.classList.add('active2')\">\n<p><a class=\"box-shade\"  style=\"background-color: rgba(0,0,0,0);\"><\/a><br \/>\n<img style=\" width: 440px; height: 240px; object-fit: cover;\" src=\"\/anatneuro\/files\/2021\/11\/image3.png\" alt=\"\"><br \/>\n<span style=\"color: #000000; position: absolute; z-index: 3; font-weight: normal; letter-spacing: 1px;\" class=\"button-text\">Identify early drivers of neuronal pathology<\/span><\/p>\n<div class=\"button-backgrounds2\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p><!-- ___________________Connection... Popup___________________ --><\/p>\n<div class=\"wrapper1\">\n<div class=\"popup\">\n<div class=\"popup-inside\">\n<div class=\"backgrounds\">\n<div class=\"background\"><\/div>\n<div class=\"background background2\"><\/div>\n<div class=\"background background3\"><\/div>\n<div class=\"background background4\"><\/div>\n<div class=\"background background5\"><\/div>\n<div class=\"background background6\"><\/div>\n<\/div>\n<\/div>\n<div class=\"content1\">\n<div class=\"content1-wrapper\">\n<p style=\"text-align: right; margin-top: -50px; margin-right: -50px;\" onclick=\"document.body.classList.remove('active')\"><i class=\"close fa fa-times fa-4x\"><\/i><\/p>\n<div class=\"p-content\">\n<h2 class=\"pop\"><a class=\"news\" href=\"#\">Map cellular diversity within the EC across species.<\/h2>\n<p><\/a><\/p>\n<p>Using different technologies (multiplexed <em>in situ<\/em> hybridization, single-nucleus RNAseq, <em>in situ<\/em> sequencing) and different species (mouse, monkey, human), we want to identify subtypes of ECII neurons, beyond the traditional distinction between lateral and medial EC.<\/p>\n<p><img src=\"\" alt=\"\"\/><\/p>\n<p><b>t-SNE plot of excitatory neurons in the mouse EC<\/b><\/p>\n<p><img src=\"\/anatneuro\/files\/2021\/11\/image1.jpg\" alt=\"\"\/><\/p>\n<p><b><em>In situ<\/em> hybridization for three markers for different subtypes of lateral ECII neurons<\/b><\/p>\n<p>We want to test the molecular profiles, the connectivity patterns, and synaptic properties of these subtypes.<br \/>\nWe also want to compare these subtypes across species.<br \/>\nNeurofibrillary tangle pathology does not start uniformly in this very heterogeneous region. It is thus indispensable to understand these neuron populations in their full complexity.<\/p>\n<p><img src=\"\/anatneuro\/files\/2022\/08\/macaqueEC.jpg\" alt=\"macaque EC\"\/><\/p>\n<p><b>Detection of ECII neurons in the macaque EC<\/b><\/p>\n<p style=\"text-align: center;\" class=\"try-again\" onclick=\"document.body.classList.remove('active')\">Back<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p><!-- ___________________Single... Popup___________________ --><\/p>\n<div class=\"popup2\">\n<div class=\"popup-inside2\">\n<div class=\"backgrounds2\">\n<div class=\"background2\"><\/div>\n<div class=\"background2 background22\"><\/div>\n<div class=\"background2 background32\"><\/div>\n<div class=\"background2 background42\"><\/div>\n<div class=\"background2 background52\"><\/div>\n<div class=\"background2 background62\"><\/div>\n<\/div>\n<\/div>\n<div class=\"content2\">\n<div class=\"content2-wrapper\">\n<p style=\"text-align: right; margin-top: -50px; margin-right: -50px;\" onclick=\"document.body.classList.remove('active2')\"><i class=\"close fa fa-times fa-4x\"><\/i><\/p>\n<div class=\"p-content\">\n<h2 class=\"pop\"><a class=\"news\" href=\"#\">Identify early drivers of neuronal pathology.<\/h2>\n<p><\/a><\/p>\n<p>Our goal is to identify genes, that very early on during the disease process in vulnerable neurons, are responsible to pathological changes culminating in neurodegeneration.<br \/>\nWe are thus looking for genes that are both:<\/p>\n<div style=\"text-align: center;\">\n<p style=\"margin-left: 50px; margin-right: 50px;\">\n        &#8211;  dysregulated transcriptionally at early presymptomatic stages of the disease. For this we use single-nucleus RNAseq and spatial transcriptomics on postmortem human EC.<br \/>\n        &#8211;  predicted to be functionally associated with tau pathology as per our functional network analysis (Roussarie et al., Neuron, 2020)<\/p>\n<\/div>\n<p>We recently identified two genes, the splice factor PTBP1 and the proto-oncogene DEK that can regulate tau in ECII neurons (respectively the splicing of tau exon 10, and the total levels of tau). We are currently investigating if these genes are dysregulated in early stages of the disease.<\/p>\n<p><img src=\"\/anatneuro\/files\/2021\/11\/image8.png\" alt=\"\"\/><\/p>\n<p><b>UMAP dimensionality reduction plot for single-nucleus RNAseq samples from control and presymptomatic AD EC.<\/b><\/p>\n<p>We are modulating the top gene candidates in the EC of AD mouse models, or in our EC in vitro systems to validate that they are indeed driving tau pathology.<\/p>\n<p>We are modulating the top gene candidates in the EC of AD mouse models, or in our EC in vitro systems to validate that they are indeed driving tau pathology.<\/p>\n<p style=\"text-align: center;\" class=\"try-again\" onclick=\"document.body.classList.remove('active2')\"\">Back<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p><!-- ___________________Modeling... Popup___________________ --><\/p>\n<div class=\"popupC\">\n<div class=\"popup-insideC\">\n<div class=\"backgroundsC\">\n<div class=\"backgroundC\"><\/div>\n<div class=\"backgroundC background2C\"><\/div>\n<div class=\"backgroundC background3C\"><\/div>\n<div class=\"backgroundC background4C\"><\/div>\n<div class=\"backgroundC background5C\"><\/div>\n<div class=\"backgroundC background6C\"><\/div>\n<\/div>\n<\/div>\n<div class=\"contentC\">\n<div class=\"contentC-wrapper\">\n<p style=\"text-align: right; margin-top: -50px; margin-right: -50px;\" onclick=\"document.body.classList.remove('activeC')\"><i class=\"close fa fa-times fa-4x\"><\/i><\/p>\n<div class=\"p-content\">\n<h2 class=\"pop\"><a class=\"news\" href=\"#\">Develop an <em>in vitro<\/em> model for vulnerable neurons.<\/h2>\n<p><\/a><\/p>\n<p>Research on selective neuronal vulnerability in Alzheimer\u2019s is slowed down by the absence of good <em>in vitro<\/em> models for these very vulnerable neurons.<\/p>\n<p>We optimized the culture of primary EC neurons from mouse embryos and used these cultures for testing vulnerability gene candidates.<\/p>\n<p><img src=\"\/anatneuro\/files\/2021\/11\/image6.jpg\" alt=\"\"\/><\/p>\n<p><b>mouse primary neuron culture from EC after silencing of a candidate vulnerability gene. Phosphorylated tau is labeled in green.<\/b><\/p>\n<p>In collaboration with Dr. Yao (Rice University), we are also searching for transcription factors that could drive ECII identity, using ECII-specific molecular profiles at different times during ECII development, as well human transcriptomic data.<\/p>\n<p>We are using candidate transcription factors in induced pluripotent stem cells, and in transdifferentiating fibroblasts to make ECII-like neurons.<\/p>\n<p><img src=\"\/anatneuro\/files\/2021\/11\/image2.jpg\" alt=\"\"\/><\/p>\n<p><b>human iPSC-derived neurons labeled with MAP2 (cyan).<\/b><\/p>\n<p style=\"text-align: center;\" class=\"try-again\" onclick=\"document.body.classList.remove('activeC')\"\">Back<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"row\">\n<div class=\"col-md-12\">\n<img src=\"\/anatneuro\/files\/2022\/06\/R_lab.jpg\" style=\"width: 50%; height: 50%;\" class=\"info-img\">\n<\/div>\n<\/div>\n<p><!-- ___________________Lab Members___________________ --><\/p>\n<div class=\"blur\">\n<article class=\"content__inner\" style=\"margin-top: 50px;\">\n<h1 class=\"content__title\">Lab Members<\/h1>\n<h3 class=\"content__author\">As of 6\/10\/22<\/h3>\n<\/article>\n<div class=\"row\">\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2021\/11\/jp2.png');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Jean-Pierre Roussarie PhD<\/h5>\n<p>Assistant Professor &#8211; <a href=\"\/anatneuro\/files\/2022\/06\/CV_roussarie_June2022.doc\">CV<\/a> &#8211; <a href=\"mailto:jproussa@bu.edu?subject = Feedback&#038;body = Message\">Contact<\/a><\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2022\/06\/Manas-scaled.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Manas Dhanuka<\/h5>\n<p>Masters Student<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2022\/06\/Irena-scaled.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Irena Feng<\/h5>\n<p>MD-PhD Student<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2021\/11\/saiyan.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Saiyan Joseph<\/h5>\n<p>BU Undergraduate Student<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2021\/11\/myles-e1636842400115.png');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Myles Joyce<\/h5>\n<p>Masters Student<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2022\/06\/Han.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Han Kahvecioglu<\/h5>\n<p>BU Undergraduate Student<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2022\/06\/ana-scaled.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Ana Morello Megias<\/h5>\n<p>PhD Student<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-3\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2022\/06\/Muriel-scaled.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Muriel Ruppert<\/h5>\n<p>STaRS Student<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-6\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2022\/06\/yang-scaled.jpg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Emily Yang<\/h5>\n<p>Lab Manager<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<div class=\"col-lg-6\">\n<div class=\"property-card\">\n<div class=\"property-image\" style=\"background-image:url('\/anatneuro\/files\/2021\/11\/Patricia_Rodriguez.jpeg');\">\n<div class=\"property-image-title\">\n        <\/div>\n<\/p><\/div>\n<div class=\"property-description\">\n<h5>Patricia Rodriguez-Rodriguez PhD<\/h5>\n<p style=\"margin-top: -3px; line-height: normal;\">Key Collaborator<br \/>Assistant Professor \u2013 Karolinska Institute<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<\/div>\n<p><!-- ___________________News___________________ --><\/p>\n<div class=\"blur\">\n<article class=\"content__inner\" style=\"margin-top: 50px;\">\n<h1 class=\"content__title\">Roussarie Lab News<\/h1>\n<h3 class=\"content__author\">From Twitter<\/h3>\n<\/article>\n<div class=\"row\" style=\"margin: auto; padding-top: 30px; padding-bottom: 30px;\">\n<div class=\"col-lg-12\" style=\"\">\n<div style=\"width: 60%; margin: auto;\">\n<a style=\"width: 60%\" class=\"twitter-timeline\" href=\"https:\/\/twitter.com\/RoussarieLab?ref_src=twsrc%5Etfw\">Tweets by RoussarieLab<\/a><br \/>\n<script async src=\"https:\/\/platform.twitter.com\/widgets.js\" charset=\"utf-8\"><\/script>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p><!-- ___________________Resources___________________ --><\/p>\n<div class=\"blur\">\n<article class=\"content__inner\" style=\"margin-top: 50px;\">\n<h1 class=\"content__title\">Resources<\/h1>\n<h3 class=\"content__author\"><\/h3>\n<\/article>\n<div class=\"row\" style=\"margin: auto; padding-top: 30px; padding-bottom: 30px;\">\n<div class=\"col-lg-4\">\n<div class=\"button2\" style=\"margin: auto; margin-top: 20px; margin-bottom: 20px; background-color: #ffffff;\">\n<p><a class=\"box-shade\" href=\"http:\/\/alz.princeton.edu\/\" style=\"background-color: rgba(0,0,0,0);\"><\/a><br \/>\n<img style=\" width: 440px; height: 240px; object-fit: cover;\" src=\"\/anatneuro\/files\/2021\/11\/jppic-1.png\" alt=\"\"><br \/>\n<span style=\"color: #ffffff; position: absolute; z-index: 3; font-weight: normal; letter-spacing: 1px;\" class=\"button-text\"><a style=\"color: #ffffff; text-decoration: none;\" href=\"http:\/\/alz.princeton.edu\/\">Neuron-type specific profiles<br \/>\nand functional networks<\/a><\/span><\/p>\n<div class=\"button-backgrounds2\"><\/div>\n<\/div>\n<\/div>\n<div class=\"col-lg-4\">\n<div class=\"button2\" style=\"margin: auto; margin-top: 20px; margin-bottom: 20px; background-color: #ffffff;\">\n<p><a class=\"box-shade\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE151460\" style=\"background-color: rgba(0,0,0,0);\"><\/a><br \/>\n<img style=\" width: 440px; height: 240px; object-fit: cover;\" src=\"\/anatneuro\/files\/2021\/11\/banner-scaled.jpg\" alt=\"\"><br \/>\n<span style=\"color: #ffffff; position: absolute; z-index: 3; font-weight: normal; letter-spacing: 1px;\" class=\"button-text\">GEO: neuron-type specific profiles<\/span><\/p>\n<div class=\"button-backgrounds2\"><\/div>\n<\/div>\n<\/div>\n<div class=\"col-lg-4\">\n<div class=\"button2\" style=\"margin: auto; margin-top: 20px; margin-bottom: 20px; background-color: #ffffff;\">\n<p><a class=\"box-shade\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE151356\" style=\"background-color: rgba(0,0,0,0);\"><\/a><br \/>\n<img style=\" width: 440px; height: 240px; object-fit: cover;\" src=\"\/anatneuro\/files\/2021\/11\/banner-scaled.jpg\" alt=\"\"><br \/>\n<span style=\"color: #ffffff; position: absolute; z-index: 3; font-weight: normal; letter-spacing: 1px;\" class=\"button-text\">GEO: Effect of PTBP1 overexpression<\/span><\/p>\n<div class=\"button-backgrounds2\"><\/div>\n<\/div>\n<\/div>\n<div class=\"col-lg-12\">\n<div class=\"button2\" style=\"margin: auto; margin-top: 20px; margin-bottom: 20px; background-color: #ffffff;\">\n<p><a class=\"box-shade\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE203368\" style=\"background-color: rgba(0,0,0,0);\"><\/a><br \/>\n<img style=\" width: 440px; height: 240px; object-fit: cover;\" src=\"\/anatneuro\/files\/2021\/11\/banner-scaled.jpg\" alt=\"\"><br \/>\n<span style=\"color: #ffffff; position: absolute; z-index: 3; font-weight: normal; letter-spacing: 1px;\" class=\"button-text\">GEO: Effect of DEK modulation<\/span><\/p>\n<div class=\"button-backgrounds2\"><\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"r-body row\">\n<div class=\"heading\">\n<h1 style=\"color: #ffffff; padding-top: 10vh;\"><a style=\"color: #ffffff; text-decoration: none;\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/myncbi\/1HGxg62aKMf\/bibliography\/public\/\">Recent Publications<\/a><\/h1>\n<\/div>\n<div class=\"container22\" style=\"overflow: auto; height: 500px;\">\n<div class=\"list\">\n<div class=\"num\"><a href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2022.05.14.491965v1\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Rodriguez-Rodriguez P, Arroyo-Garcia L, Li L, Tsagkogianni C, Wang W, Salas-Allende I, Plautz Z, Cedazo-Minguez A, Sinha S, Troyanskaya O, Flajolet M, Yao V, Roussarie J. The proto-oncogene DEK regulates neuronal excitability and tau accumulation in Alzheimer\u2032s disease vulnerable neurons. bioRxiv. 2022 May; :2022.05.14.491965. doi: 10.1101\/2022.05.14.491965.<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/35177825\/\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Montalban E, Giralt A, Taing L, Schut EHS, Supiot LF, Castell L, Nakamura Y, de Pins B, Pelosi A, Goutebroze L, Tuduri P, Wang W, Neiburga KD, Vestito L, Castel J, Luquet S, Nairn AC, Herv\u00e9 D, Heintz N, Martin C, Greengard P, Valjent E, Meye FJ, Gambardella N, Roussarie JP, Girault JA. Translational profiling of mouse dopaminoceptive neurons reveals region-specific gene expression, exon usage, and striatal prostaglandin E2 modulatory effects. Mol Psychiatry. 2022 Apr;27(4):2068-2079. doi: 10.1038\/s41380-022-01439-4. Epub 2022 Feb 18. PubMed PMID: 35177825.<br \/>\n<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/www.biorxiv.org\/content\/10.1101\/2022.03.09.483648v1\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Goikolea J, Roussarie J, Gerenu G, Loera-Valencia R, Latorre-Leal M, Cedazo-Minguez A, Rodriguez-Rodriguez P, Maioli S. Neuron-derived Thioredoxin-80: a novel regulator of type-I interferon response in microglia. 2022\/01; :2022.03.09.483648. doi: 10.1101\/2022.03.09.483648.<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32603655\/\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Roussarie JP, Yao V, Rodriguez-Rodriguez P, Oughtred R, Rust J, Plautz Z, Kasturia S, Albornoz C, Wang W, Schmidt EF, Dannenfelser R, Tadych A, Brichta L, Barnea-Cramer A, Heintz N, Hof PR, Heiman M, Dolinski K, Flajolet M, Troyanskaya OG, Greengard P. Selective Neuronal Vulnerability in Alzheimer&#8217;s Disease: A Network-Based Analysis. Neuron. 2020 Sep 9;107(5):821-835.e12. doi: 10.1016\/j.neuron.2020.06.010. Epub 2020 Jun 29. PubMed PMID: 32603655; PubMed Central PMCID: PMC7580783.<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Montalban E, Giralt A, Taing L, Nakamura Y, Martin C, de Pins B, Pelosi A, Goutebroze L, Castell L, Wang W, Daila Neiburga K, Vestito L, Nairn AC, Valjent E, Herv\u00e9 D, Heintz N, Gambardella le Nov\u00e8re N, Greengard P, Roussarie J, Girault J. Translational profiling of mouse dopaminoceptive neurons reveals a role of PGE2 in dorsal striatum. [preprint]. 2020 September. doi: https:\/\/doi.org\/10.1101\/2020.09.02.279240.<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Roussarie J, Rodriguez-Rodriguez P. Deciphering cell-type specific signal transduction in the brain: Challenges and promises. In: Advances in Pharmacology San Diego: Academic Press; 2020. Chapter 7; p.145-171.<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26214373\/\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Brichta L, Shin W, Jackson-Lewis V, Blesa J, Yap EL, Walker Z, Zhang J, Roussarie JP, Alvarez MJ, Califano A, Przedborski S, Greengard P. Identification of neurodegenerative factors using translatome-regulatory network analysis. Nat Neurosci. 2015 Sep;18(9):1325-33. doi: 10.1038\/nn.4070. Epub 2015 Jul 27. PubMed PMID: 26214373; PubMed Central PMCID: PMC4763340.<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25720483\/\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Vidalain PO, Jacob Y, Hagemeijer MC, Jones LM, Neveu G, Roussarie JP, Rottier PJ, Tangy F, de Haan CA. A field-proven yeast two-hybrid protocol used to identify coronavirus-host protein-protein interactions. Methods Mol Biol. 2015;1282:213-29. doi: 10.1007\/978-1-4939-2438-7_18. PubMed PMID: 25720483; PubMed Central PMCID: PMC7121825.<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/19779566\/\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Brahic M, Roussarie JP. Axon-myelin interactions during a viral infection of the central nervous system. PLoS Pathog. 2009 Sep;5(9):e1000519. doi: 10.1371\/journal.ppat.1000519. Epub 2009 Sep 25. PubMed PMID: 19779566; PubMed Central PMCID: PMC2743189.<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/18159229\/\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Roussarie JP, Ruffi\u00e9 C, Edgar JM, Griffiths I, Brahic M. Axon myelin transfer of a non-enveloped virus. PLoS One. 2007 Dec 26;2(12):e1331. doi: 10.1371\/journal.pone.0001331. PubMed PMID: 18159229; PubMed Central PMCID: PMC2137932.<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/17305428\/\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Roussarie JP, Ruffi\u00e9 C, Brahic M. The role of myelin in Theiler&#8217;s virus persistence in the central nervous system. PLoS Pathog. 2007 Feb;3(2):e23. doi: 10.1371\/journal.ppat.0030023. PubMed PMID: 17305428; PubMed Central PMCID: PMC1797621.<\/h3>\n<p>    <\/a><\/div>\n<div class=\"num\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/15542687\/\"><\/p>\n<h3 style=\"font-weight: normal; text-indent: -4em; margin-left: 4em;\">Mena I, Roussarie JP, Brahic M. Infection of macrophage primary cultures by persistent and nonpersistent strains of Theiler&#8217;s virus: role of capsid and noncapsid viral determinants. J Virol. 2004 Dec;78(23):13356-61. doi: 10.1128\/JVI.78.23.13356-13361.2004. PubMed PMID: 15542687; PubMed Central PMCID: PMC525035.<\/h3>\n<p><\/a><\/div>\n<\/p><\/div>\n<\/div>\n<\/div>\n<div class=\"row\">\n<div class=\"col-md-4\">\n<div id=\"tv\">\n<div class=\"universal-padding\">\n<h2 class=\"tv-title\">Contact Us<\/h2>\n<p class=\"contacts\">Roussarie Lab<\/p>\n<p class=\"contacts\">72 East Concord Street, L817<\/p>\n<p class=\"contacts\">Boston, MA 02115<\/p>\n<p class=\"contacts\">Phone: 617-358-0811<\/p>\n<p class=\"contacts\">Email: jproussa@bu.edu<\/p>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"col-md-4\">\n<div id=\"tv\">\n<div class=\"universal-padding\">\n<h2 class=\"tv-title\">Alumni<\/h2>\n<p class=\"contacts\"><b>Postdocs:<\/b><br \/>\n<br \/>Patricia Rodriguez-Rodriguez 2018-2021<\/p>\n<p class=\"contacts\"><b>Research Assistants:<\/b><br \/>\n<br \/>Isabella Salas-Allende 2019-2021<br \/>\n<br \/>Zakary Plautz 2017-2019<br \/>\n<br \/>Mallory Brown 2015-2017<br \/>\n<br \/>Assia Mouri 2014-2015<br \/>\n<br \/>Quigly Dragotakes 2014-2016<br \/>\n<br \/>Christian Albornoz 2011-2013<br \/>\n<br \/>Shirin Kasturia 2010-2011<\/p>\n<p class=\"contacts\"><b>Undergraduate student:<\/b><br \/>Sophia Andreadis 2016, 2019<\/p>\n<p class=\"contacts\">\n<p class=\"contacts\">\n<p class=\"contacts\">\n<\/div>\n<\/div>\n<\/div>\n<div class=\"col-md-4\">\n<div id=\"tv\">\n<div class=\"universal-padding\">\n<h2 class=\"tv-title\">Funding Sources<\/h2>\n<p>  <img loading=\"lazy\" style=\"margin-bottom: 20px;\" src=\"\/anatneuro\/files\/2021\/11\/NIA_logo.jpeg\" alt=\"\" width=\"577\" height=\"87\" class=\"alignnone size-full wp-image-13266\" \/><br \/>\n<img loading=\"lazy\" style=\"margin-bottom: 20px;\" src=\"\/anatneuro\/files\/2021\/11\/Cure_Alzheimers_Fund_logo-scaled.jpeg\" alt=\"\" width=\"577\" height=\"87\" class=\"alignnone size-full wp-image-13266\" \/>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<p><!-- ___________________Page CSS___________________ --><\/p>\n<style>\n<p>html, body {\n    width: 100%;\n    height: 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0;\n}<\/p>\n<p>body.activeC .backgroundC{\n    transform: scale(1);\n}<\/p>\n<p>body.activeC .background6C{\n    transform: scale(8);\n}<\/p>\n<\/style>\n","protected":false},"excerpt":{"rendered":"<p>Roussarie Lab Roussarie Lab Our Research: Selective neuronal vulnerability: leveraging this underexplored property of Alzheimer\u2019s, to molecularly dissect the disease Like almost all neurodegenerative diseases, Alzheimer\u2019s disease in its earliest stages only affects very specific sets of neurons. In the majority of patients, neurons from the layer II of entorhinal cortex (ECII) are the first [&hellip;]<\/p>\n","protected":false},"author":18550,"featured_media":0,"parent":0,"menu_order":6,"comment_status":"closed","ping_status":"closed","template":"page-templates\/no-sidebars.php","meta":[],"_links":{"self":[{"href":"https:\/\/www.bumc.bu.edu\/anatneuro\/wp-json\/wp\/v2\/pages\/10106"}],"collection":[{"href":"https:\/\/www.bumc.bu.edu\/anatneuro\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bumc.bu.edu\/anatneuro\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/anatneuro\/wp-json\/wp\/v2\/users\/18550"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bumc.bu.edu\/anatneuro\/wp-json\/wp\/v2\/comments?post=10106"}],"version-history":[{"count":50,"href":"https:\/\/www.bumc.bu.edu\/anatneuro\/wp-json\/wp\/v2\/pages\/10106\/revisions"}],"predecessor-version":[{"id":12670,"href":"https:\/\/www.bumc.bu.edu\/anatneuro\/wp-json\/wp\/v2\/pages\/10106\/revisions\/12670"}],"wp:attachment":[{"href":"https:\/\/www.bumc.bu.edu\/anatneuro\/wp-json\/wp\/v2\/media?parent=10106"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}