Production of Transgenic Mice

PRODUCTION OF TRANSGENIC MICE

As indicated in the Schematic Overview below, transgenic mice are produced by injecting DNA of interest into one-cell embryos followed by implantation into foster mice. This procedure is commonly performed on FVB mice, but can be performed on a variety of mouse strains. The gestation period of a mouse is approximately 3 weeks, and pups are ready for weaning and tail biopsies (for DNA analysis of transgene integration) at 3 weeks of age. Females reach maturity at 6 weeks and males at 8 weeks.

The facility will inject the DNA no less than 250 eggs, which will be implanted into foster mice , 90% of which will become pregnant. The investigator will be notified of the number of pups born, and all those would be potential transgenic founders. * The facility will transfer the potential founders to the investigator at the age of three weeks. It will be the responsibility of the investigator to obtain tail biopsies, or to arrange for the core to perform the task, and to analyze for transgene integration within two weeks. At that time, the caring and breeding of the transgenic mice will be under the responsibility of the investigator.

Boston University School of Medicine/Transgenic Knock Out Core Facility


Scientific Director of Transgenic/Knock out Core, Dr. Katya Ravid
Transgenic Specialist and Manager, Greg Martin

Primary teaching affiliate
of BU School of Medicine