Lines produced at BUSM

Transgenic and Knock Out Lines Produced at BUSM

Please note: The table below lists many of the transgenic (TG) and knock out (KO) lines produced at BUSM. We trust that this collection will allow effective sharing of resources and information. Other lines produced by the core, and which are not listed here, did not yield a phenotype, or are under further investigation. It is well known that not all integrated transgenes are properly expressed, dependent upon the integration site. In cases where a line integrated the transgene, but did not express it in more than 4 founders, a reconstructed DNA (or the same, if appropriate) was re-injected. Further information may be obtained from the core or directly from the investigators.

Investigator E-mail TG or KO Construct Name cDNA or genomic Comment on gene in focus Year received Mouse strain
Abedi, Merhdad mabedi@rwmc.org TG c-Kit-TetR Promotor C-kit, TetR gene. 2006 FVB
Amar, Solomon samar@bu.edu KO LITAF clone #80 LITAF gene 2003 129Sv cells, C57BL/6 background
Botchkarev, Vladimir vladbotc@bu.edu TG hK5-Flag-Noggin cDNA human K5 promoter driving noggin gene 2000 C3H/HeJ
- TG hVER-Flag-Noggin cDNA human Versican promoter driving noggin gene 2000 C3H/HeJ
- TG hK5-Flag-Noggin cDNA human K5 promoter driving noggin gene 2004 FVB
- TG K14Alk3QD Promotor keratin14,gene Alk3 /Cdna 2006 FVB
- TG
K14LhX2
 Promotor keratin14,Lhx2 gene 2006 FVB
TG TRP2ERT2Cre 2008 FVB
Cardoso, Wellington wcardoso@bu.edu TG SP-C-Wnt7b cDNA SP-C promotor driving Wnt-7b gene 1998 C57BL/6
- TG Spc-RAR-VP16a SPC promoter driving RARaVP16 gene 2000 FVB
- TG Spc-RAR-ca cDNA SPC promoter driving RARa1 b-galactosidase gene 2000 FVB
- TG SP-C-RARbVP16 cDNA SP-C promoter driving RARb-VP16 gene 2000 FVB
- TG KSP-cdh U6 snRNA promotor driving siRNA for ksp-cdh gene 2003 FVB
- KO HS6ST1-loxP Knocking out Heparin Sulfate 6-0-sulfotransferase (HS6ST1) gene; two loxP sites flanking Exon1 of HS6ST1 gene 2005 129Sv cells, C57BL/6 background
Tg SpC-MSulf2-HA SpC driving MSulf2 producing
mouse heparan sulfate 6-0-endosulfatase2		 2008 FVB
Center, David collaborative project with H. Kornfeld dcenter@bu.edu KO Il-16 genomic DNA Interleukin-16 gene 1999 129Sv cells, C57BL/6 background
Chen, Kai collaborative project with J. Keaney kaichen@bu.edu TG pTRE2-Nox4-V5 cDNA CMV promotor driving Nox4 gene 2004 FVB
Cohen, Herbert htcohen@bu.edu TG nephrin3kb cDNA and genomic DNA nephrin promoter driving luciferase gene 2000 FVB
Cohen, Richard collaborative project with K. Walsh racohen@bu.edu KO a2-AMPK cDNA Tie 2 promoter driving dominant negative AMP-activated protein kinase 2003 129Sv cells, C57BL/6 background
Cruikshank, William bcruiksh@bu.edu TG CD4-C-prepro-FLAG-secreted IL-16 cDNA mouse CD4-enhancer-human CD4 promoter/5′UTR-IL-16 2002 BALB/c
Denis, Gerald gdenis@bu.edu TG RING 3 WT genomic DNA IgG enh (murine & human) promoter driving RING3 gene 1999 FVB
- TG RING 3 K/A genomic DNA IgG enh (murine & human) promoter driving RING3 gene (K/A) 1999 FVB
- KO Gene Brd2 gene knocked out 2007 C57B16 strain
Fine, Alan afine@bu.edu TG sma-GFP genomic DNA Rat smooth muscle actin promoter driving GFP gene 2005 C57BL/6
Foster, Judith foster@biochem.bumc.bu.edu TG 2.2 MSE cDNA tropoelastin promoter driving CAT gene 1997 FVB
Gavras, Haralambos hgavras@bu.edu TG a-2B PDGFb promoter driving a-2B gene 2002 FVB
- TG a-2B PDGFb promoter driving a-2B gene 2005 FVB
Gerstenfeld, Louis lgersten@bu.edu TG CMV-TF cDNA CMV promoter driving chimeric TF 2003 FVB
- TG pPL-2-BMP BMP promoter driving native TF 2003 FVB
- TG Luciferase Target Construct cDNA Artificial promotor driving luciferase gene 2004 B6D2F1
- TG CMV-TF(2) cDNA CMV promoter driving chimeric TF 2005 FVB
- TG BMP2 Target Construct cDNA IL-2 promoter driving BMP2 gene 2005 C57BL/6
TG Noggin 2008 C57Bl/6
Gustavo, Mostoslavsky jkeaney@bu.edu KO SEF8 2009 C57Bl/6-Tyr
Gyurko, Robert gyurko@bu.edu TG ChemR23TG Promotor CD11b,chemR23 gene. 2006 FVB
Joyce-Brady, Martin mjbrady@bu.edu TG 3.7SPC/SV40, cDNA SPC promotor driving BI-1 gene 1998 FVB
- TG Spc-TEGT+ cDNA 1999 FVB
Keaney, John collaborative project with K. Chen jkeaney@bu.edu TG pTRE2-Nox4-V5 cDNA CMV promotor driving Nox4 gene 2004 FVB
Kirsch, Kathrin kirschk@bu.edu TG p130Cas / BCAR1 cDNA MMTV-LTR/virus promoter driving BCAR1 gene 2003 FVB
Kornfeld, Hardy collaborative project with D. Center hkornfeld@lung.bumc.bu.edu KO Il-16 genomic DNA Interleukin-16 gene 1999 129Sv cells, C57BL/6 background
Lafyatis, Robert
collaborative project with R. Lemaire		 lafyatis@bu.edu TG pTRE2 1 MAGP-2 cDNA CMV-1 promotor driving MAGP-2 gene 2004 FVB
Lemaire, Raphael
collaborative project with R. Lafyatis		 rlemaire@bu.edu TG pTRE2 1 MAGP-2 cDNA CMV-1 promotor driving MAGP-2 gene 2004 FVB
Lerner, Adam lernwara@bu.edu KO clone 91 2002 129Sv cells, C57BL/6 background
- KO clone 42 2002 129Sv cells, C57BL/6 background
Liao, Ronglih TG IDCM-TnC C1 cDNA a-myosin heavy chain murine promoter driving troponin C gene 2001 FVB
- TG TNC(2) cDNA a-myosin heavy chain murine promoter driving troponin C gene 2001 FVB
- TG TNC(3) cDNA a-myosin heavy chain murine promoter driving troponin C gene 2003 FVB
Little, Frederic flittle@bu.edu TG CD4-C-prepro-FLAG-secreted IL-16 cDNA mouse CD4-enhancer-human CD4 promoter/5′UTR 2005 FVB
Loscalzo, Joseph Fotini Vavva was responsible for this project. She is no longer at BUSM. KO gpx KO clones #5 & #8 G6PDH 2002 129Sv cells, C57BL/6 background
Marshak-Rothstein, Ann collaborative project with R. Lafyatis amrothst@bu.edu TG pTRE-tGO cDNA tetracycline responsive element promoter driving tGO gene 2005 BALB/c
Pilch, Paul ppilch@bu.edu KO CAVIN KO VectorpPNT 2006 C57BL/6
Polgar, Peter peterp@bu.edu TG AB3T cDNA b actin promoter driving rat angiotensin 2 receptor with atr sequences replaced by BKR sequences in 2 places 2005 FVB C57BL/6 KO for BKB2R will be used for backcross
Ravid, Katya ravid@biochem.bumc.bu.edu TG PF4D3GH genomic DNA PF4 promotor driving Cyclin D3 gene 1996 FVB
- TG Aim1 cDNA rat PF4 promoter driving rat Aim1 cDNA 2002 FVB
- TG P2Y12 cDNA P2Y12 in platelets 2002 FVB
- KO NA-3 (ES 07) Tbv-2 derived clone of Mst1 KO 2002 129Sv cells, C57Bl/6 background
- KO A2bAR gene knock out knock out of the A2b adenosine receptor 2004 129Sv cells, C57Bl/6 background
- TG pB1-survivin bi-directional Tet-responsive-Element driving Survivin (birc5)(mouse) gene 2004 FVB
- TG PF4-Globin H2BGFP (Histone) rat PF4 promoter driving Histone fused to GFP 2005 FVB
- TG PF4-TubulinYFP rat PF4 promoter driving Tubulin fused to YFP 2005 FVB
TG Cyclin 2008 FVB
TG CD68A2b-/- 2008 A2b-/-
TG Pf4-pacgfp1-tubulin 2009 FVB
Rothstein, Thomas tr@bu.edu TG pIg TEN/STAT3 cDNA IgH promoter driving faim gene 1996 FVB
- TG PIg TeN (STAT3 dominant negative) cDNA Ig promoter driving STAT3 dominant negative gene 1997 FVB
- TG pIgTEN (STAT3) cDNA Ig mu promoter driving STAT3 gene 1997 FVB
- TG pIg Te/NEO (STAT3b) cDNA Immunoglobin promoter driving Stat3b gene 1997 FVB
- TG CMV-FAIM-S cDNA CMV promoter driving faim-s gene 1999 FVB
- TG CMV-FAIM-L cDNA CMV promoter driving faim-L gene 1999 FVB
- TG pIgTEN (STAT3) M10 cDNA Ig mu promoter driving STAT3 gene 1999 FVB
- KO pKO 919 (ES 369) cDNA NO promoter, faim gene 2001 129Sv cells, C57BL/6 background
Seldin, David dseldin@bu.edu TG MMTV SV40 gskb cDNA LTR promoter driving GSKb gene 1996 FVB
- TG MMTV gskb Mut cDNA LTR promoter driving GSKb Mut gene 1996 FVB
- KO pIGTE/n CK2a’ genomic DNA NO promoter murine CK2a’ gene 1998 129Sv cells, C57BL/6 background
- TG CK2a’ cDNA human Ig promoter driving CK2a’ gene 2001 FVB
- TG pcDNA3 CMV viral promoter driving the human lambdaimmunoglobulin light chain 2004 FVB
Sherr, David dsherr@bu.edu TG EmPm-AhR cDNA Ig promotor driving AhR gene 1996 C57BL/6
- TG EmPm-AhR cDNA Ig promotor driving AhR gene 2000 C57BL/6
- TG MMTV-AhR cDNA MMTV promotor driving AhR gene 1998 C57BL/6
- TG MMTV-NLS-AhR cDNA MMTV promotor driving AhR gene 2002 C57BL/6
- TG pNLS MMTV-AhR flag cDNA MMTV promoter driving AhR-FLAG 2002 FVB
Smith, Barbara smith@biochem.bumc.bu.edu TG COL CAT 3.6 cDNA rat 3.6kb Collagen +/- mutation driving CAT gene 1995 C57BL/6
- TG COL CAT 3.6 cDNA rat 3.6kb Collagen +/- mutation driving CAT gene 1995 FVB
- TG mCOL CAT 3.6 cDNA rat 3.6kb Collagen +/- mutation driving CAT gene 1995 FVB
Sonenshein, Gail gsonensh@bu.edu TG c-Rel cDNA MMTV promoter driving c-Rel gene 1998 FVB
- TG 1Kba cDNA MMTV promoter driving 1kba 32/3bA gene 1998 FVB
- TG B-myb cDNA CMV promoter driving B-myb gene 1998 FVB
Spanjaard, Remco rspan@bu.edu TG pTYRcyclinD cDNA tyrosine (mouse) promoter driving cyclinD gene 2000 FVB
Strehlow, David TG PN1 cDNA CMV promoter driving Protease Nexin 1 gene 1999 FVB
Walsh, Kenneth collaborative project with R. Cohen kxwalsh@bu.edu KO a2-DNAmpk cDNA Tie 2 promoter driving dominant negative AMP-activated protein kinase 2003 129Sv cells, C57BL/6 background
TG (#1) Sca-1-tTA IRES GFP cDNA Sca-1 (mouse) promoter driving tTA & GFP genes 2003 FVB
- TG (#2) Tet-c.a.AFX (aka AFXEnR) cDNA Tetracycline-responsive promoter driving constitutively active AFX 2003 FVB
- TG (#3) Tet-d.n.AFX (aka AFX3A) cDNA Tetracycline-responsive promotor driving dominant negative AFX gene 2003 FVB
TG MCKFstl1 2008 C57Bl/6
TG MCK-Insl6 2009 C57Bl/6
Williams, Mary marywill@bu.edu TG 1.3kb T1a-CAT, cDNA short T1a promotor, driving CAT gene 1995 FVB
- TG 10kb T1a-CAT, cDNA long T1a promotor, driving CAT gene 1997 FVB
- KO T1a-KO genomic DNA KO of T1a murine gene 2001 129Sv cells, C57BL/6 background
- TG 10kb T1a-GFP cDNA 10kb T1a promoter driving GFP gene 2001 FVB
Wolfe, Michael mwolfe@bu.edu TG pEGFP-193 genomic DNA short GIP promoter driving GFP gene 1998 FVB
- TG pEGFP-2512 genomic DNA long GIP promoter driving GFP gene 1998 FVB
- TG pGLacZ-2512 genomic DNA GIP promoter driving b-galactosidase gene 1998 FVB
- TG pGLacZ-193 genomic DNA GIP promoter driving b-galactosidase gene 1998 FVB
- TG pEGFP-193 genomic DNA GIP promoter driving b-galactosidase gene 1998 FVB
Wu, Mei KO pNTR LacZ PGK neo-IEX-1 genomic DNA pNTR LacZ PGK neo-IEX-1 2003 129Sv cells, C57BL/6 background
Xiao, Jim jxiao@biochem.bumc.bu.edu TG Spc-HA-E2F1DN cDNA TRE promotor driving E2F1DN gene 2000 FVB
- TG Spc-HA-p130 cDNA Spc promotor driving p130 gene 1999 FVB
Yu, Qiang TG spc-Tet cDNA SP-C promoter driving tTA gene 1998 FVB
Zannis, Vassilis vzannis@bu.edu TG CMVE2 cDNA CMV promoter driving dapoE2 gene 1995 C57BL/6
- TG CMVE2 cDNA MT-1 promoter driving dapoE2 gene 1995 C57BL/6
- TG pSPORT-1CIIIP-LacZ-AI genomic DNA apo CIII and apo AI promoters driving apo AI gene 1996 FVB
- TG pSPORT-hPDGF-apoE2g gemonic DNA PDGF promoter driving apoE2g gene 1997 C57BL/6
- TG CIIImut AI (wt) genomic DNA CIII and AI promotors, driving apoAI gene 1998 C57BL/6
- TG AImut CIII genomic DNA CIII and AI promotors driving apoAI gene 1998 C57BL/6
- TG PDGF cDNA PDGF-B promoter driving App gene 2000 C57BL/6
- TG C/EBP genomic DNA apoliprotein promoter driving apoliprotein gene 2001 C57BL/6
- TG B+C+D genomic DNA apoliprotein promoter driving apoliprotein gene 2001 C57BL/6
- TG G genomic DNA apoliprotein promoter driving apoliprotein An gene 2002 C57BL/6
Zhang, Ying zhangyin@bu.edu TG PUC-MAR-sig-gal genomic DNA PF4 promotor driving gal gene 1996 FVB
- TG PF4-UPA genomic DNA PF4 promotor driving PF4-UPA gene 1996 FVB
- TG Myc in Megakaryocytes Accepted by Jackson Labs 1996 FVB
- TG PF4-D1-GH genomic DNA Cyclin D1 in Megak 1998 FVB
- TG PF4-Bclx BclxL in Megak 1999 FVB
- TG PF4-Bclx-5′UTR PF4 promoter driving Bclx-L (5′UTR) gene 1999 FVB
- TG PF4-P2Y1-hGH cDNA P2Y1 in Platelets 1999 FVB
- TG PSA3Z A3 adenosine receptor driving b-gal 1999 FVB
- TG pPF4-Tet cDNA rat PF4 promoter driving bacterial Tet enhance xVP16 2002 FVB
- TG pTet-Aim1 minimal CMV-tet promoter (bi-directional) driving b-gal and AIM-1 (Aurora-B) 2001 FVB

Mouse Strain Table
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