Lines produced at BUSM

Transgenic and Knock Out Lines Produced at BUSM

Please note: The table below lists many, but NOT ALL, of the transgenic (TG) and knock out (KO) lines produced at BUSM. We trust that this collection will allow effective sharing of resources and information. Other lines produced by the core, and which are not listed here, did not yield a phenotype (although the transgene or KO development were successful), or are under further investigation, or were not permitted by the PI to be published here. It is well known that not all integrated transgenes are properly expressed, dependent upon the integration site.


Investigator E-mail TG or KO Construct Name cDNA or genomic Comment on gene in focus Year Mouse strain
Ellen Weinberg eweinbe@bu.edu TG/KO IL33-/-Apoe Apoe Tg breed with IL33-/- 2012 C57Bl6
Orian Shirihai shirihai@bu.edu KO MFN2 Mitofusin Knock in 2012 B6-Tyr
Rutao Cui rutaocui@bu.edu TG Tg Flag MC1R Gene MC1R; Gene protein coupled receptor 2012 e/e
Ting C Zhao tzhao@bu.edu TG HDAC4 cardiac contractility 2012 FVB
Katya Ravid kravid@bu.edu TG PF4 Statin Gene:VE-Statin/Egfl7; inhibit catalytic activity of lysyl oxidase through protein-protein interaction 2012 Gata-1 Low
Paul Pilch ppilch@bu.edu KO Adrx Info at: http://www.velocigene.com/komp/detail/15730 2012 B6-Tyr
Gerald Denis gdenis@bu.edu KO Brd2LoxP Gene Brd2 knocked out;homologous recombination of LoxP targeting vector 2012 B6
Botchkarev, Vladimir vladbotc@bu.edu TG Tre Satb1 TREtight promoter 2011 FVB
Guo, Wen wguo@bu.edu TG dnR2B pTrre-tight vector,Promoter Tre 2011 C57Bl6
Ravid, Katya kravid@bu.edu TG PF4-Lox 2011 FVB
Zhao, Ting tzhao@bu.edu TG p38B alpha-MHC promoter driving p38B gene 2011 FVB
Cui, Rutao rutaocui@bu.edu TG Actin-Pax3 chicken B-actin promoter/Pax3 2010 C57Bl6
Cui, Rutao rutaocui@bu.edu TG Mitf chicken B-actin/Mitf 2010 C57BL6
Ikezu, Tsuneya tikezu@bu.edu TG CamK-APPtet Promotor CamKIIalpha, APP/swe/Ind gene. 2010 C57Bl6 & NOD Skid
Morin, Peter TG WR2 pUC57 vector 2010 C57Bl6
Morin, Peter TG NR2 pUC57 vector 2010 C57Bl6
Morin, Peter TG HR2 pUC57 vector 2010 C57Bl6
Morin, Peter TG SR2 pUC57 vector 2010 C57Bl6
Naya, Francisco fnaya@bu.edu TG LoxP myospryn shRNA promoter:H1RNAPolIII gene: shRNA 2010 FVB
Seldin, David dseldin@bu.edu TG VK*Myc-F2A-L6 VK* promotes hMyc, patient IDL6(rearranged) 2010 C57BL/6 background
Seldin, David dseldin@bu.edu TG VK*Myc-F2A-K1 VK* promotes hMyc, patient IGK1(rearranged) 2010 C57BL/6 background
Walsh, Kenneth kxwalsh@bu.edu TG ap2-Sfrp5 ap2 promoter driving Sfrp5 gene 2010 C57Bl/6
Weinberg, Ellen eweinbe@bu.edu KO IL33 KO Velocigene KOMP Design BAC-Vec 2010 B6-Tyr
Zhang, Ying zhangyin@bu.edu TG p Tight-p38 P-Tie-Tight-p38 construct, P-Tre-CMV promoter, P38 gene 2010 FVB
Zhao, Ting tzhao@bu.edu TG PUC-MAR-sig-gal genomic DNA PF4 promotor driving gal gene 2010 FVB
Cohen, Richard racohen@bu.edu TG Flagsirt SIRT1 gene 2009 C57BL/6 background
Gustavo, Mostoslavsky jkeaney@bu.edu KO SEF8 2009 C57Bl/6-Tyr
Kotton, Darrell Darrel.Kotton@bmc.org KO TTF1-GFP-SPC-dsRed 2009 B6
Lu, Jining jining@bu.edu KO TNRC6a TNRC6a 2009 C57BL/6 background
Lu, Jining jining@bu.edu KO TNRC6b TNRC6b 2009 C57BL/6 background
Lu, Jining jining@bu.edu KO TNRC6c TNRC6c 2009 C57BL/6 background
Gustavo, Mostoslavsky gmostosl@bu.edu KO iPs SEF8 2009 C57Bl/6-Tyr
Pilch, Paul ppilch@bu.edu KO Prkcdbp KO 2009 B6Tyr
Ravid, Katya kravid@bu.edu TG CD68A2b-/- 2009 A2b-/-
Ravid, Katya kravid@bu.edu TG Pf4-pacgfp1-tubulin vector PF4-b globin,PF4 promoter 2009 FVB
Ravid, Katya kravid@bu.edu TG PF43XGFP-EMBT PF4 promoter 2009 FVB
Toth, Louis ljtoth@bu.edu TG CAG-Lox-GFP- 2009 B6
Toth, Louis ljtoth@bu.edu TG CTRC-A73T 2009 B6
Walsh, Kenneth kxwalsh@bu.edu TG MCK-Insl6 2009 C57Bl/6
Botchkarev, Vladimir vladbotc@bu.edu TG TRP2ERT2Cre Vector:pBS,Dct gene 2008 FVB
Cardoso, Wellington wcardoso@bu.edu TG SpC-MSulf2-HA SpC driving MSulf2 producing mouse heparan sulfate 6-0-endosulfatase2 2008 FVB
Gerstenfeld, Louis lgersten@bu.edu TG Noggin 2008 C57Bl/6
Ravid, Katya kravid@bu.edu TG Cyclin 2008 FVB
Walsh, Kenneth kxwalsh@bu.edu TG MCKFstl1 MCK promoter,vector:pBSMCK 2008 C57Bl/6
Denis, Gerald gdenis@bu.edu KO Gene Brd2 gene knocked out 2007 C57BL6
Abedi, Merhdad mabedi@rwmc.org TG c-Kit-TetR Promotor C-kit, TetR gene. 2006 FVB
Botchkarev, Vladimir vladbotc@bu.edu TG K14Alk3QD Promotor keratin14,gene Alk3 /Cdna 2006 FVB
Botchkarev, Vladimir vladbotc@bu.edu TG K14LhX2 Promotor keratin14,Lhx2 gene 2006 FVB
Gyurko, Robert gyurko@bu.edu TG ChemR23TG Promotor CD11b,chemR23 gene. 2006 FVB
Pilch, Paul ppilch@bu.edu KO CAVIN 3 KO VectorpPNT 2006 C57BL/6
Cardoso, Wellington wcardoso@bu.edu KO HS6ST1-loxP Knocking out Heparin Sulfate 6-0-sulfotransferase (HS6ST1) gene; two loxP sites flanking Exon1 of HS6ST1 gene 2005 129Sv cells, C57BL/6 background
Fine, Alan afine@bu.edu TG sma-GFP genomic DNA Rat smooth muscle actin promoter driving GFP gene 2005 C57BL6
Gavras, Haralambos hgavras@bu.edu TG a-2B PDGFb promoter driving a-2B gene 2005 FVB
Gerstenfeld, Louis lgersten@bu.edu TG CMV-TF(2) cDNA CMV promoter driving chimeric TF 2005 FVB
Gerstenfeld, Louis lgersten@bu.edu TG BMP2 Target Construct cDNA IL-2 promoter driving BMP2 gene 2005 C57BL/6
Little, Frederic flittle@bu.edu TG CD4-C-prepro-FLAG-secreted IL-16 cDNA mouse CD4-enhancer-human CD4 promoter/5′UTR 2005 FVB
Marshak-Rothstein, Ann collaborative project with R. Lafyatis amrothst@bu.edu TG pTRE-tGO cDNA tetracycline responsive element promoter driving tGO gene 2005 BALB/c
Polgar, Peter peterp@bu.edu TG AB3T cDNA b actin promoter driving rat angiotensin 2 receptor with atr sequences replaced by BKR sequences in 2 places 2005 FVB C57BL/6 KO for BKB2R will be used for backcross
Ravid, Katya kravid@bu.edu TG PF4-Globin H2BGFP (Histone) rat PF4 promoter driving Histone fused to GFP 2005 FVB
Ravid, Katya kravid@bu.edu TG PF4-TubulinYFP rat PF4 promoter driving Tubulin fused to YFP 2005 FVB
Botchkarev, Vladimir vladbotc@bu.edu TG hK5-Flag-Noggin cDNA human K5 promoter driving noggin gene 2004 FVB
Chen, Kai kaichen@bu.edu TG pTRE2-Nox4-V5 cDNA CMV promotor driving Nox4 gene 2004 FVB
Gerstenfeld, Louis lgersten@bu.edu TG Luciferase Target Construct cDNA Artificial promotor driving luciferase gene 2004 B6D2F1
Keaney, John collaborative project with K. Chen jkeaney@bu.edu TG pTRE2-Nox4-V5 cDNA CMV promotor driving Nox4 gene 2004 FVB
Lafyatis, Robert lafyatis@bu.edu TG pTRE2 1 MAGP-2 cDNA CMV-1 promotor driving MAGP-2 gene 2004 FVB
Lemaire, Raphael rlemaire@bu.edu TG pTRE2 1 MAGP-2 cDNA CMV-1 promotor driving MAGP-2 gene 2004 FVB
Ravid, Katya kravid@bu.edu KO A2bAR gene knock out knock out of the A2b adenosine receptor 2004 129Sv cells, C57Bl/6 background
Ravid, Katya kravid@bu.edu TG pB1-survivin bi-directional Tet-responsive-Element driving Survivin (birc5)(mouse) gene 2004 FVB
Seldin, David dseldin@bu.edu TG pcDNA3 CMV viral promoter driving the human lambdaimmunoglobulin light chain 2004 FVB
Amar, Solomon samar@bu.edu KO LITAF clone #80 LITAF gene 2003 129Sv cells, C57BL/6 background
Cardoso, Wellington wcardoso@bu.edu TG KSP-cdh U6 snRNA promotor driving siRNA for ksp-cdh gene 2003 FVB
Cohen, Richard racohen@bu.edu KO a2-AMPK cDNA Tie 2 promoter driving dominant negative AMP-activated protein kinase 2003 129Sv cells, C57BL/6 background
Gerstenfeld, Louis lgersten@bu.edu TG CMV-TF cDNA CMV promoter driving chimeric TF 2003 FVB
Gerstenfeld, Louis lgersten@bu.edu TG pPL-2-BMP BMP promoter driving native TF 2003 FVB
Kirsch, Kathrin kirschk@bu.edu TG p130Cas / BCAR1 cDNA MMTV-LTR/virus promoter driving BCAR1 gene 2003 FVB
Liao, Ronglih TG TNC(3) cDNA a-myosin heavy chain murine promoter driving troponin C gene 2003 FVB
Walsh, Kenneth/Cohen kxwalsh@bu.edu KO a2-DNAmpk cDNA Tie 2 promoter driving dominant negative AMP-activated protein kinase 2003 129Sv cells, C57BL/6 background
Walsh, Kenneth kxwalsh@bu.edu TG (#1) Sca-1-tTA IRES GFP cDNA Sca-1 (mouse) promoter driving tTA & GFP genes 2003 FVB
Walsh, Kenneth kxwalsh@bu.edu TG (#2) Tet-c.a.AFX (aka AFXEnR) cDNA Tetracycline-responsive promoter driving constitutively active AFX 2003 FVB
Walsh, Kenneth kxwalsh@bu.edu TG (#3) Tet-d.n.AFX (aka AFX3A) cDNA Tetracycline-responsive promotor driving dominant negative AFX gene 2003 FVB
Cruikshank, William bcruiksh@bu.edu TG CD4-C-prepro-FLAG-secreted IL-16 cDNA mouse CD4-enhancer-human CD4 promoter/5′UTR-IL-16 2002 BALB/c
Gavras, Haralambos hgavras@bu.edu TG a-2B PDGFb promoter driving a-2B gene 2002 FVB
Lerner, Adam lernwara@bu.edu KO clone 91 2002 129Sv cells, C57BL/6 background
Lerner, Adam lernwara@bu.edu KO clone 42 2002 129Sv cells, C57BL/6 background
Loscalzo, Joseph Fotini Vavva was responsible for this project. She is no longer at BUSM. KO gpx KO clones #5 & #8 G6PDH 2002 129Sv cells, C57BL/6 background
Ravid, Katya kravid@bu.edu TG Aim1 cDNA rat PF4 promoter driving rat Aim1 cDNA 2002 FVB
Ravid, Katya kravid@bu.edu TG P2Y12 cDNA P2Y12 in platelets 2002 FVB
Ravid, Katya kravid@bu.edu KO NA-3 (ES 07) Tbv-2 derived clone of Mst1 KO 2002 129Sv cells, C57Bl/6 background
Sherr, David dsherr@bu.edu TG MMTV-NLS-AhR cDNA MMTV promotor driving AhR gene 2002 C57BL/6
Sherr, David dsherr@bu.edu TG pNLS MMTV-AhR flag cDNA MMTV promoter driving AhR-FLAG 2002 FVB
Zannis, Vassilis vzannis@bu.edu TG G genomic DNA apoliprotein promoter driving apoliprotein An gene 2002 C57BL/6
Zhang, Ying zhangyin@bu.edu TG pPF4-Tet cDNA rat PF4 promoter driving bacterial Tet enhance xVP16 2002 FVB
Liao, Ronglih TG IDCM-TnC C1 cDNA a-myosin heavy chain murine promoter driving troponin C gene 2001 FVB
Liao, Ronglih TG TNC(2) cDNA a-myosin heavy chain murine promoter driving troponin C gene 2001 FVB
Rothstein, Thomas tr@bu.edu KO pKO 919 (ES 369) cDNA NO promoter, faim gene 2001 129Sv cells, C57BL/6 background
Seldin, David dseldin@bu.edu TG CK2a’ cDNA human Ig promoter driving CK2a’ gene 2001 FVB
Williams, Mary marywill@bu.edu KO T1a-KO genomic DNA KO of T1a murine gene 2001 129Sv cells, C57BL/6 background
Williams, Mary marywill@bu.edu TG 10kb T1a-GFP cDNA 10kb T1a promoter driving GFP gene 2001 FVB
Zannis, Vassilis vzannis@bu.edu TG C/EBP genomic DNA apoliprotein promoter driving apoliprotein gene 2001 C57BL/6
Zannis, Vassilis vzannis@bu.edu TG B+C+D genomic DNA apoliprotein promoter driving apoliprotein gene 2001 C57BL/6
Botchkarev, Vladimir vladbotc@bu.edu TG hK5-Flag-Noggin cDNA human K5 promoter driving noggin gene 2000 C3H/HeJ
Botchkarev, Vladimir vladbotc@bu.edu TG hVER-Flag-Noggin cDNA human Versican promoter driving noggin gene 2000 C3H/HeJ
Cohen, Herbert htcohen@bu.edu TG nephrin3kb cDNA and genomic DNA nephrin promoter driving luciferase gene 2000 FVB
Cardoso, Wellington wcardoso@bu.edu TG Spc-RAR-VP16a SPC promoter driving RARaVP16 gene 2000 FVB
Cardoso, Wellington wcardoso@bu.edu TG Spc-RAR-ca cDNA SPC promoter driving RARa1 b-galactosidase gene 2000 FVB
Cardoso, Wellington wcardoso@bu.edu TG SP-C-RARbVP16 cDNA SP-C promoter driving RARb-VP16 gene 2000 FVB
Xiao, Jim jxiao@biochem.bumc.bu.edu TG Spc-HA-E2F1DN cDNA TRE promotor driving E2F1DN gene 2000 FVB
Sherr, David dsherr@bu.edu TG EmPm-AhR cDNA Ig promotor driving AhR gene 2000 C57BL/6
Spanjaard, Remco rspan@bu.edu TG pTYRcyclinD cDNA tyrosine (mouse) promoter driving cyclinD gene 2000 FVB
Zannis, Vassilis vzannis@bu.edu TG PDGF cDNA PDGF-B promoter driving App gene 2000 C57BL/6
Center, David dcenter@bu.edu KO Il-16 genomic DNA Interleukin-16 gene 1999 129Sv cells, C57BL/6 background
Denis, Gerald gdenis@bu.edu TG RING 3 WT genomic DNA IgG enh (murine & human) promoter driving RING3 gene 1999 FVB
Denis, Gerald gdenis@bu.edu TG RING 3 K/A genomic DNA IgG enh (murine & human) promoter driving RING3 gene (K/A) 1999 FVB
Joyce-Brady, Martin mjbrady@bu.edu TG Spc-TEGT+ cDNA 1999 FVB
Kornfeld, Hardy collaborative project with D. Center hkornfeld@lung.bumc.bu.edu KO Il-16 genomic DNA Interleukin-16 gene 1999 129Sv cells, C57BL/6 background
Kornfeld, Hardy collaborative project with D. Center hkornfeld@lung.bumc.bu.edu KO Il-16 genomic DNA Interleukin-16 gene 1999 129Sv cells, C57BL/6 background
Rothstein, Thomas tr@bu.edu TG CMV-FAIM-S cDNA CMV promoter driving faim-s gene 1999 FVB
Rothstein, Thomas tr@bu.edu TG CMV-FAIM-L cDNA CMV promoter driving faim-L gene 1999 FVB
Rothstein, Thomas tr@bu.edu TG pIgTEN (STAT3) M10 cDNA Ig mu promoter driving STAT3 gene 1999 FVB
Strehlow, David TG PN1 cDNA CMV promoter driving Protease Nexin 1 gene 1999 FVB
Xiao, Jim jxiao@biochem.bumc.bu.edu TG Spc-HA-p130 cDNA Spc promotor driving p130 gene 1999 FVB
Zhang, Ying zhangyin@bu.edu TG PF4-Bclx BclxL in Megak 1999 FVB
Zhang, Ying zhangyin@bu.edu TG PF4-Bclx-5′UTR PF4 promoter driving Bclx-L (5′UTR) gene 1999 FVB
Zhang, Ying zhangyin@bu.edu TG PF4-P2Y1-hGH cDNA P2Y1 in Platelets 1999 FVB
Zhang, Ying zhangyin@bu.edu TG PSA3Z A3 adenosine receptor driving b-gal 1999 FVB
Cardoso, Wellington wcardoso@bu.edu TG SP-C-Wnt7b cDNA SP-C promotor driving Wnt-7b gene 1998 C57BL/6
Joyce-Brady, Martin mjbrady@bu.edu TG 3.7SPC/SV40, cDNA SPC promotor driving BI-1 gene 1998 FVB
Seldin, David dseldin@bu.edu KO pIGTE/n CK2a’ genomic DNA NO promoter murine CK2a’ gene 1998 129Sv cells, C57BL/6 background
Sherr, David dsherr@bu.edu TG MMTV-AhR cDNA MMTV promotor driving AhR gene 1998 C57BL/6
Sonenshein, Gail gsonensh@bu.edu TG c-Rel cDNA MMTV promoter driving c-Rel gene 1998 FVB
Sonenshein, Gail gsonensh@bu.edu TG 1Kba cDNA MMTV promoter driving 1kba 32/3bA gene 1998 FVB
Sonenshein, Gail gsonensh@bu.edu TG B-myb cDNA CMV promoter driving B-myb gene 1998 FVB
Wolfe, Michael mwolfe@bu.edu TG pEGFP-193 genomic DNA short GIP promoter driving GFP gene 1998 FVB
Wolfe, Michael mwolfe@bu.edu TG pEGFP-2512 genomic DNA long GIP promoter driving GFP gene 1998 FVB
Wolfe, Michael mwolfe@bu.edu TG pGLacZ-2512 genomic DNA GIP promoter driving b-galactosidase gene 1998 FVB
Wolfe, Michael mwolfe@bu.edu TG pGLacZ-193 genomic DNA GIP promoter driving b-galactosidase gene 1998 FVB
Wolfe, Michael mwolfe@bu.edu TG pEGFP-193 genomic DNA GIP promoter driving b-galactosidase gene 1998 FVB
Yu, Qiang TG spc-Tet cDNA SP-C promoter driving tTA gene 1998 FVB
Zannis, Vassilis vzannis@bu.edu TG CIIImut AI (wt) genomic DNA CIII and AI promotors, driving apoAI gene 1998 C57BL/6
Zannis, Vassilis vzannis@bu.edu TG AImut CIII genomic DNA CIII and AI promotors driving apoAI gene 1998 C57BL/6
Zhang, Ying zhangyin@bu.edu TG PF4-D1-GH genomic DNA Cyclin D1 in Megak 1998 FVB
Foster, Judith foster@biochem.bumc.bu.edu TG 2.2 MSE cDNA tropoelastin promoter driving CAT gene 1997 FVB
Rothstein, Thomas tr@bu.edu TG PIg TeN (STAT3 dominant negative) cDNA Ig promoter driving STAT3 dominant negative gene 1997 FVB
Rothstein, Thomas tr@bu.edu TG pIgTEN (STAT3) cDNA Ig mu promoter driving STAT3 gene 1997 FVB
Rothstein, Thomas tr@bu.edu TG pIg Te/NEO (STAT3b) cDNA Immunoglobin promoter driving Stat3b gene 1997 FVB
Williams, Mary marywill@bu.edu TG 10kb T1a-CAT, cDNA long T1a promotor, driving CAT gene 1997 FVB
Zannis, Vassilis vzannis@bu.edu TG pSPORT-hPDGF-apoE2g gemonic DNA PDGF promoter driving apoE2g gene 1997 C57BL/6
Ravid, Katya kravid@bu.edu TG PF4D3GH genomic DNA PF4 promotor driving Cyclin D3 gene 1996 FVB
Rothstein, Thomas tr@bu.edu TG pIg TEN/STAT3 cDNA IgH promoter driving faim gene 1996 FVB
Sherr, David dsherr@bu.edu TG EmPm-AhR cDNA Ig promotor driving AhR gene 1996 C57BL/6
Seldin, David dseldin@bu.edu TG MMTV SV40 gskb cDNA LTR promoter driving GSKb gene 1996 FVB
Seldin, David dseldin@bu.edu TG MMTV gskb Mut cDNA LTR promoter driving GSKb Mut gene 1996 FVB
Zannis, Vassilis vzannis@bu.edu TG pSPORT-1CIIIP-LacZ-AI genomic DNA apo CIII and apo AI promoters driving apo AI gene 1996 FVB
Zhang, Ying zhangyin@bu.edu TG PUC-MAR-sig-gal genomic DNA PF4 promotor driving gal gene 1996 FVB
Zhang, Ying zhangyin@bu.edu TG PF4-UPA genomic DNA PF4 promotor driving PF4-UPA gene 1996 FVB
Zhang, Ying zhangyin@bu.edu TG Myc in Megakaryocytes Accepted by Jackson Labs 1996 FVB
Smith, Barbara smith@biochem.bumc.bu.edu TG COL CAT 3.6 cDNA rat 3.6kb Collagen +/- mutation driving CAT gene 1995 C57BL/6
Smith, Barbara smith@biochem.bumc.bu.edu TG COL CAT 3.6 cDNA rat 3.6kb Collagen +/- mutation driving CAT gene 1995 FVB
Smith, Barbara smith@biochem.bumc.bu.edu TG mCOL CAT 3.6 cDNA rat 3.6kb Collagen +/- mutation driving CAT gene 1995 FVB
Williams, Mary marywill@bu.edu TG 1.3kb T1a-CAT, cDNA short T1a promotor, driving CAT gene 1995 FVB
Zannis, Vassilis vzannis@bu.edu TG CMVE2 cDNA CMV promoter driving dapoE2 gene 1995 C57BL/6
Zannis, Vassilis vzannis@bu.edu TG CMVE2 cDNA MT-1 promoter driving dapoE2 gene 1995 C57BL/6

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Mouse Strain Table

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