Introduction to the Transgenic and Genome Engineering Core

CRISPR/Cas9-mediated knockout of the Tyrosinase (albino) gene in C57BL/6J mice.

The TgGE Core is a full-service core that provides state-of-the-art transgenic and genome engineering services to enable the production of animal models of human disease. We work closely with investigators to design and execute experiments based on their needs and provide fully customizable support to enable the successful completion of the investigator’s experimental aims. We strive to be the local resource for developing, planing, executing, and analyzing experiments involving genetically engineered mice. The TgGE Core has a long history of successfully generating mouse models and we look forward to building on this record by continually expanding and updating the services offered.

Chimeric mice generated by injecting R1 (Agouti) ES cells into C57BL/6J (non-agouti) blastocysts.

Chimeric mice generated by injecting R1 (Agouti) ES cells into C57BL/6J (non-agouti) blastocysts.


The TgGE can assist you with generating transgenic mice by pronuclear microinjection into zygotes and genetically engineered mice by microinjection of ES cells into blastocyst stage embryos. Gene editing can be performed using TALENs or CRISPR/Cas9-mediated approaches. We offer services to secure your valuable lines by cryopreservation of sperm and or embryos and we can resurrect these lines using in vitro fertilization or embryo transfer. Additional services include rederivation to create pathogen-free mouse lines, ES cell manipulation, developing molecular reagents for genetic engineering, colony management, and expert advice on mouse genetics and breeding schemes.


For more information or to inquire about customized services please feel free to contact us.

Primary teaching affiliate
of BU School of Medicine