Protocols
ES Cells | iPS Cells | Vectors
Embryonic Stem Cell Culture Protocols
- Mouse ESC Media with Serum
- Serum free ESC maintenance media
- ESC Passage
- Thawing and Freezing ES Cells
- Transduction of mESC with Lentivirus
- Making Mouse Embryonic Fibroblasts (MEFs)
- Shipping Tail Tips
- Mouse ESC differentiation to Nkx2-1+ lung and thyroid progenitors (Bio-Protocol)
- 2i Media (Feeder Free, Serum Free)
iPS Cell Culture Protocols
- Human Dermal Fibroblast Isolation
- Isolation of Peripheral Blood Mononuclear Cells for Reprogramming
- Mouse iPS Cell Generation with STEMCCA
- Human iPS Cell Generation with hSTEMCCA
- Human iPS Cell Generation from Peripheral Blood with hSTEMCCA
- Southern Blot with human gDNA to screen for hSTEMCCA integration in hiPS cells
Vector Protocols
- Protocol 1: Lentivirus Packaging by 293T Transfection
- Protocol 2: Calculations for Transfection of 293T for Lenti/Retro Packaging
- Protocol 3: Titering Lentivirus on FG293 Cells
- Protocol 4: Titering Lentiviral Vectors by Southern Blot
- Protocol 5: Retrovirus Packaging
- Protocol 6: Primer Design for Cloning Genes into the pHAGE Lentiviral Backbone
- Protocol 7: Bacterial Storage and Glycerol Stocks
- Protocol 8: Lentiviral Transduction of Hematopoietic Stem Cells
- Protocol 9: Lentiviral Transduction of Alveolar Macrophages by Intratracheal Injection
**The Boston University Center for Regenerative Medicine endorses the International Stem Cell Registry (U.Mass), and The National Stem Cell bank (WiCell; University of Wisconsin). Detailed human ES culture protocols and cell banks are maintained at each of these sites; click below to access their highly recommended protocol pages:
