See the original publication in Cell Stem Cell: “Efficient Derivation of Purified Lung and Thyroid Progenitors from Embryonic Stem Cells.”

See the article in BU Today!

CReM Investigators Publish Research into Differentiation Capacity of iPSC

See the press release!

See the original publication in the Journal of Clinical Investigation: “Mouse ES and iPS cells can form similar definitive endoderm despite differences in imprinted genes.”

CReM Directors Profiled in Bostonia

CReM Investigators publish humanized version of the novel Stem Cell Reprogramming Vector “STEMCCA”

click here to link to the publication in Stem Cells

CReM Investigators derive the first iPS cells specific for lung disease, from individuals with Cystic Fibrosis or alpha-1 antitrypsin deficiency-related emphysema

Click here to link to the Stem Cells journal publication

MGH and CReM investigators engineer bioartificial rat lung

Investigators used a mild detergent to remove resident cells from a harvested rat lung, leaving a white scaffold that was reseeded with endothelial and epithelial cells.  This bioartificial lung was characterized in vitro and successfully transplanted into a rat.  Read press releases at Boston.com, Reuters, and PhysOrg.com.

CReM Investigators publish novel approach to gene therapy for pulmonary emphysema

Life-long gene expression in lung tissue achieved by in vivo lentiviral transduction of long-lived pulmonary alveolar macrophages.  Press releases can be found at U.S. News & World Report and Science Blog.
Click here to view the Journal of Clinical Investigation publication of the method.

ARRA stimulus grant from the NIH Awarded to CReM: RC2 ‘Grand Opportunities (GO)’ Grant to study iPS Cells

CReM researchers have been awarded an RC2 GO grant to derive differentiated endodermal and hematopoietic lineages from human iPS cells.
The multicenter study, lead by Boston University Principal Investigators, Drs. Darrell Kotton and Gustavo Mostoslavsky, applies reprogramming technology to derive human iPS cells and then characterize their differentiated progeny using bioinformatics approaches. Click here to read the NIH press release.

On-line summaries of the latest publications this year from CReM investigators:

Cardoso Lab on journal cover

• Amelioration of emphysema in mice through lentiviral transduction of long-lived pulmonary alveolar macrophages. Wilson AA, Murphy GJ, Hamakawa H, Kwok LW, Srinivasan S, Hovav AH, Mulligan RC, Amar S, Suki B, and Kotton DN. Journal of Clinical Investigation. 2009. Epub Dec 21 doi:10.1172/JCI36666.
• Excision of Reprogramming Transgenes Improves the Differentiation Potential of iPS Cells Generated with a Single Excisable Vector. Sommer CA, Gianotti Sommer A, Longmire TA, Christodoulou C, Thomas DD, Gostissa M, Alt FW, Murphy GJ, Kotton DN, Mostoslavsky G. Stem Cells. 2009 Nov 10. [Epub ahead of print]
• Differentiation stage determines potential of hematopoietic cells for reprogramming into induced pluripotent stem cells. Eminli, S., Foudi, A., Stadtfeld, M., Maherali, N., Ahfeldt, T. Mostoslavsky, G., Hock, H., Hochedlinger, K. Nature Genetics. 2009. Epub August 9 doi:10.1038/ng.428. PMID: 19668214 [PubMed - as supplied by publisher]
• Transcriptional analysis of fracture healing and the induction of embryonic stem cell-related genes. Bais M, McLean J, Sebastiani P, Young M, Wigner N, Smith T, Kotton DN, Einhorn TA, Gerstenfeld LC. PLoS One. 2009;4(5):e5393. Epub 2009 May 5.PMID: 19415118 [PubMed - indexed for MEDLINE]
• Notch signaling controls the balance of ciliated and secretory cell fates in developing airways. Tsao PN, Vasconcelos M, Izvolsky KI, Qian J, Lu J, Cardoso WV. Development. 2009 Jul;136(13):2297-307. PMID: 19502490 [PubMed - in process]
• Induced pluripotent stem cell generation using a single lentiviral stem cell cassette. Sommer CA, Stadtfeld M, Murphy GJ, Hochedlinger K, Kotton DN, Mostoslavsky G. Stem Cells. 2009 Mar;27(3):543-9. PMID: 19096035 [PubMed - in process]
• Breathing life into the lung stem cell field. Fine A. Cell Stem Cell. 2009 Jun 5;4(6):468-9. PMID: 19497272 [PubMed - in process]

Mouse iPS Cell Colonies