Use the IVIS
Getting started with in vivo imaging:
1) To determine project feasibility, discuss your experimental goals with:
a) the Core Manager, Anna Studwell (astudwel@bu.edu), R-205, x4-1654
b) or one of the Core Directors: Lou Gerstenfeld, Ph.D., lgersten@bu.edu , Darrell Kotton, M.D., dkotton@bu.edu
2) Complete the necessary trainings and complete/submit the IVIS Core Training Form to the Core Manager
- Note that the final 1-hour core training with the Core Manager cannot be completed until all previous trainings are done
- Pending manager approval, you will receive swipe-card access to W816 upon completion of this final training
3) For live animal work, complete or update an IACUC protocol to detail your experimental design
- Specify use of the IVIS camera and procedures to be performed on mice in W816 (IVIS location)
- Attach the IVIS Standard Operating Procedures (SOP) to this protocol: here
- For ex vivo imaging, discuss your experiment with the Core Manager
4) Obtain a username/password or update/add grant information for the online core resource scheduler by filling out the form at the following address: http://www.bmcmedicine.info/CoreDevices.html
-Note that EVERY new user must obtain a separate username/password
5) Sign up for imaging time! To schedule this device: click here (approved user login required)
6) The IVIS is available 24/7 for user access including weekends. However, keep in mind that:
a) troubleshooting assistance and maintenance will not be available after 5 or on weekends/holidays
b) it is the user’s responsibility to pay for any damage he/she may have caused to the machine/room
while imaging (this excludes normal wear and tear)
c) if the machine is not functioning properly or other issues arise, it is the user’s responsibility to
contact Core Director, Louis Gerstenfeld via e-mail lgersten@bu.edu who will then shut down access to the room until the technician returns.
d) For after-hours clearance, please fill out the form below:
Online Resource Scheduler Use
1) Once you have obtained a username/password for the online resource scheduler, sign up for imaging time in W816 by clicking links: ”Animal Core” followed by “Xenogen IVIS Imaging System Series”.
2) If you would like techinician assistance anytime while imaging, request this in the slot “Assistance required”
- If you would like assistance, please reserve your time at least one day ahead of time
- If you have requested assistance, please try to be punctual as the technician will wait no longer than 15 minutes, and your account will still be charged for the reserved time if no notification is received:
* If it is 5 minutes or more BEFORE your scheduled time, contact the technician at 4-1654
* If it is AFTER your scheduled time, contact the technician at the W816 phone at 4-1647
3) Sign up for analysis time (for use with the software alone) at 650 Albany St, EBRC building 4th floor, Murphy Lab
4) Currently, the time reserved on the online resource scheduler (and not necessarily the time that you actually spend imaging) is billed at a rate of $35/hour and $50/hour with assistance.
*Please Contact Anna Studwell with any questions
