Forms and Getting Started with IVIS
Getting started with in vivo imaging checklist
| Steps to developing an IVIS protocol | Resource | Forms to fill out |
| Contact Core Director or Manager | Director/Manager Lou Gerstenfeld, lgersten@bu.edu, x4-1660
Director Darrell Kotton, dkotton@bu.edu, x8-4860 |
N/A |
| Submit IACUC protocol | http://www.bu.edu/orctraining/animal/iacuc/bumc-training/ | Attach to protocol:SOP for IVIS Animal Imaging Core |
| Access the Core scheduling system | http://wwwapp.bumc.bu.edu/BUSM/CoreDevices/ServiceCenterIntroduction.aspx | N/A |
| Complete Trainings | Contact Information for Training | N/A |
| Fill out LASC security form | http://www.bu.edu/animalcare/forms/lasc-bumc-forms/ | Security Access Application Form |
| Fill out IVIS Core training form | Submit hard copy or via e-mail to technician | IVIS Core Training Form |
| Order animals | http://www.bu.edu/animalcare/forms/lasc-bumc-forms/ | Animal Order Form |
| Sign up for imaging time | Register with the schedule:
http://wwwapp.bumc.bu.edu/BUSM/CoreDevices/ServiceCenterIntroduction.aspx To sign up for imaging time: http://coremgmt.bumc.bu.edu/ocf/admin.php |
N/A |
*For Charles River Campus Researchers: Researchers from the Charles River Campus Guidelines for In vivo Imaging Facility Use
SOP example of typical phrasing: “Animals will be imaged using an In vivo Imaging System (IVIS) Spectrum (Caliper Life Sciences, Hopkinton, MA, USA). Anesthesia is administered in an induction chamber with 2.5% isoflurane in 100% oxygen at a flow rate of 1.5 L/min and maintained while the animal is in the IVIS imaging device with a 2.5% mixture at 0.25 L/min administered via nose cones. The chamber within the imaging device is heated to maintain appropriate animal body temperature during the imaging process which can last between 5 to 45 minutes. Animals will be imaged at such and such frequency (1,2,3x daily/weekly). See attached standard operating procedures (SOP) for more details.”
*The IVIS is available 24/7 for user access including weekends. However, keep in mind that:
a) troubleshooting assistance and maintenance will not be available after 5 or on weekends/holidays
b) it is the user’s responsibility to pay for any damage he/she may have caused to the machine/room
while imaging (this excludes normal wear and tear)
c) if the machine is not functioning properly or other issues arise, it is the user’s responsibility to
contact Core Director, Louis Gerstenfeld via e-mail lgersten@bu.edu who will then shut down access to the room until the weekday schedule resumes.
