IVIS Capabilities and Publications
Boston University’s IVIS Spectrum has the ability to detect both bioluminescence and fluorescence in vivo. Researchers from many departments of the Medical Campus have been using the camera for their experiments. See below to view their research:
1) NOD/SCID mouse fat pad injected with HS578T cell line, pre-labeled with lentiviral dsRed-IRES-mTomato vector; red fluorescence image capture is shown below:
Courtesy Jillian Richmond
3) In vivo effects of BMP2 shRNA inhibition on bone formation after marrow ablation. Seen below are In vivo measurements of bioluminescent intensities from mice injected in the tibia with lentivirus overexpressing luciferase (lenti-Luc) compared to PBS injected controls.
Courtesy Manish Bais, Ph.D.
Selected Publications with BU’s IVIS
J. Richmond, M. Finkel, A. Studwell, F. Little, and W. Cruikshank (2011). Introduction of Pro-interleukin-16 inhibits T lymphoblastic leukemia growth in mice. Journal of Cancer Research and Clinical Oncology. Epub. View PDF
Wilson, AA, Murphy, GJ, Hamakawa, H, Kwok, LW, Srinivasan, S, Hovav, AH, Mulligan, RC, Amar, S, Suki, B and Kotton DN (2010). Amelioration of emphysema in mice through lentiviral transduction of long-lived pulmonary alveolar macrophages. J Clin Inv 120(1):379–389. View PDF
Bais MV, Wigner N, Young M, Toholka R, Graves DT, Morgan EF, Gerstenfeld LC, Einhorn TA (2009) BMP2 is essential for post natal osteogenesis but not for recruitment of osteogenic stem cells. Bone. 45(2): 254-66. View PDF
