Ping-Ping Kuang, M.D.

Faculty and Fellows


Research Assistant Professor of Medicine

Medical School: Hunan Medical University, Changsha, China
Internship: The 1st Medical Univ. Hospital, Changsha, China
Residency and Fellowship: Institute of Epidemiology & Microbiology, CAPM, Beijing, China
PhD programs: Biochemistry, Boston University School of Medicine.
Post-doctoral Fellowships/Training: Infectious Diseases, Dana Farber Cancer Institute, Boston; Pulmonary Center, Boston University School of Medicine, Boston

Special Interests:

  • Molecular and Cellular Biology of Elastogenesis
  • Emphysema and Lung Injury Repair

Research Overview:

Elastogenesis

is a complex process of the formation of mature elastic fibers, which is mediated and coordinated by a variety of key molecules such as fibulin-5, LOX- and LOXL-proteins to temporally and spatially deposit soluble tropoelastin on microfibrillar scaffold.

Emphysema

is a chronic lung destructive disease that is usually caused by smoking and characterized by permanent progressive distal airway enlargement and destruction of alveolar walls. Dr. Kuang’s main research interests are focused on the molecular basis of fibulin-5 and tropoelastin in elastogenesis and pathogenesis of emphysema. Using in vitro lung fibroblast cultures and in vivo mouse models, Dr. Kuang and colleagues have discovered that pro-inflammatory cytokine interleukin-1? (IL-1?) inhibits expression of elastin and fibulin-5 gene by the activation of both NF-?B and C/EBP? pathways, whereas transforming growth factor ? (TGF-?) induces elastin and fibulin-5 expression in Smads- and PI3K/Akt-signaling-dependent fashion. Current ongoing research projects are examining the effect of fibulin-5 on the cellular function and the maintenance of homeostasis of lung oxidative status using biochemical, biophysical and molecular and cellular approaches.

Selected Publications:

  1. Kuang, P. P., Zhang, X.H., Rich, C., Foster, J.A., Subramanian, M., Goldstein, R.H. (2007) ACTIVATION OF ELASTIN TRANSCRIPTION BY TRANSFORMING GROWTH FACTOR-{beta} IN HUMAN LUNG FIBROBLASTS. Am J Physiol Lung Cell Mol Physiol. (In press)
  2. Kuang, P. P., Joyce-Brady, M., Zhang, X. H., Jean, J. C. and Goldstein, R. H. (2006). Fibulin-5 gene expression in human lung fibroblasts is regulated by TGF-beta and phosphatidylinositol 3-kinase activity. Am J Physiol Cell Physiol 291, C1412-21.
  3. Subramanian, M., Kuang, P. P., Wei, L., Rishikof, D. C., Liu, H. and Goldstein, R. H. (2006). Modulation of amino acid uptake by TGF-beta in lung myofibroblasts. J Cell Biochem 99, 71-8.
  4. Rishikof, D. C., Lucey, E. C., Kuang, P. P., Snider, G. L. and Goldstein, R. H. (2006). Induction of the myofibroblast phenotype following elastolytic injury to mouse lung. Histochem Cell Biol 125, 527-34.
  5. Serlin, D. M., Kuang, P. P., Subramanian, M., O’Regan, A., Li, X., Berman, J. S. and Goldstein, R. H. (2006). Interleukin-1beta induces osteopontin expression in pulmonary fibroblasts. J Cell Biochem 97, 519-29.
  6. Rishikof, D. C., Kuang, P. P., Subramanian, M. and Goldstein, R. H. (2005). Methods for measuring type I collagen synthesis in vitro. Methods Mol Med 117, 129-40.
  7. Kuang, P. P., Lucey, E., Rishikof, D. C., Humphries, D. E., Bronsnick, D. and Goldstein, R. H. (2005). Engraftment of neonatal lung fibroblasts into the normal and elastase-injured lung. Am J Respir Cell Mol Biol 33, 371-7.
  8. Kuang, P. P. and Goldstein, R. H. (2005). Regulation of elastin gene transcription by proteasome dysfunction. Am J Physiol Cell Physiol 289, C766-73.
  9. Kuang, P. P., Goldstein, R. H., Liu, Y., Rishikof, D. C., Jean, J. C. and Joyce-Brady, M. (2003). Coordinate expression of fibulin-5/DANCE and elastin during lung injury repair. Am J Physiol Lung Cell Mol Physiol 285, L1147-52.
  10. Kuang, P. P. and Goldstein, R. H. (2003). Regulation of elastin gene transcription by interleukin-1 beta-induced C/EBP beta isoforms. Am J Physiol Cell Physiol 285, C1349-55.
  11. Kuang, P. P., Berk, J. L., Rishikof, D. C., Foster, J. A., Humphries, D. E., Ricupero, D. A. and Goldstein, R. H. (2002). NF-kappaB induced by IL-1beta inhibits elastin transcription and myofibroblast phenotype. Am J Physiol Cell Physiol 283, C58-65.
  12. Rishikof, D. C., Ricupero, D. A., Kuang, P. P., Liu, H. and Goldstein, R. H. (2002). Interleukin-4 regulates connective tissue growth factor expression in human lung fibroblasts. J Cell Biochem 85, 496-504.
  13. Lucey, E. C., Keane, J., Kuang, P. P., Snider, G. L. and Goldstein, R. H. (2002). Severity of elastase-induced emphysema is decreased in tumor necrosis factor-alpha and interleukin-1beta receptor-deficient mice. Lab Invest 82, 79-85.
  14. Ricupero, D. A., Poliks, C. F., Rishikof, D. C., Cuttle, K. A., Kuang, P. P. and Goldstein, R. H. (2001). Phosphatidylinositol 3-kinase-dependent stabilization of alpha1(I) collagen mRNA in human lung fibroblasts. Am J Physiol Cell Physiol 281, C99-C105.
  15. Ricupero, D. A., Poliks, C. F., Rishikof, D. C., Kuang, P. P. and Goldstein, R. H. (2001). Apigenin decreases expression of the myofibroblast phenotype. FEBS Lett 506, 15-21.
  16. Ricupero, D. A., Rishikof, D. C., Kuang, P. P., Poliks, C. F. and Goldstein, R. H. (1999). Regulation of connective tissue growth factor expression by prostaglandin E(2). Am J Physiol 277, L1165-71.
  17. Rishikof, D. C., Kuang, P. P., Poliks, C. and Goldstein, R. H. (1998). Regulation of type I collagen mRNA in lung fibroblasts by cystine availability. Biochem J 331 ( Pt 2), 417-22.
  18. Krupsky, M., Kuang, P. P. and Goldstein, R. H. (1997). Regulation of type I collagen mRNA by amino acid deprivation in human lung fibroblasts. J Biol Chem 272, 13864-8.
  19. Bloom, B. B., Humphries, D. E., Kuang, P. P., Fine, A. and Goldstein, R. H. (1996). Structure and expression of the promoter for the R4/ALK5 human type I transforming growth factor-beta receptor: regulation by TGF-beta. Biochim Biophys Acta 1312, 243-8.
  20. Krupsky, M., Fine, A., Kuang, P. P., Berk, J. L. and Goldstein, R. H. (1996). Regulation of type I collagen production by insulin and transforming growth factor-beta in human lung fibroblasts. Connect Tissue Res 34, 53-62.