John Bernardo, M.D.

Faculty and Fellows

Professor of Medicine, Research Professor of Biochemistry
Tuberculosis Medical Officer, Massachusetts Department of Public Health

Medical School: Univ of Illinois, Chicago
Internship: New York Hospital/Memorial Hospital
Residency: New York Hospital/Memorial Hospital; Boston City Hospital
Fellowship: NHLBI, NIH

Special Interests:


  • Basic Science Research: Control of Phagocyte Function and novel applications of Flow Cytometry/kinetic flow imaging
  • Translational-Clinical Research: Tuberculosis: diagnostics; treatment; community-based prevention; education; policy


  • Tuberculosis
  • Allergy

Clinical/Research Activities:

Dr. Bernardo is Professor of Medicine at Boston University School of Medicine. He attends on the pulmonary consult service and critical care unit at Boston Medical Center. He is active in local and national TB programmatic activities, including: National Tuberculosis Controllers’ Association (NTCA) where he has served as Executive Committee Board President in 2006-7 and 2014-15, and Stop TB USA, where he served as its Chairperson from 2010-12.  He currently is president-elect of the National Society of TB Clinicians of the NTCA. Dr. Bernardo also is the NTCA representative to the Association of Public Health Laboratories Tuberculosis Subcommittee. Dr. Bernardo is a collaborator at the CDC’s Northeast Tuberculosis Regional Training and Medical Consultation Center at Rutgers Medical School, Newark, NJ, where he teaches courses on tuberculosis and provides regional medical consultation for complex cases, and TB Medical Officer, Massachusetts Department of Public Health, Div of TB Prevention and Control. He also has served on numerous CDC advisory committees on tuberculosis.

Outside of Boston Medical Center, Dr. Bernardo is a Pulmonary/TB Physician for Boston’s Health Care for the Homeless, Inc.

Dr. Bernardo’s basic research interests include basic studies of the control of the phagovacuolar environment (human neutrophils and monomuclear phagocytes) using kinetic flow cytometry and novel flow cytometric techniques (NHLBI support), in collaboration with Elizabeth Simons, Ph.D., Professor of Biochemistry. For more details, please see our webpage of TB/HIV Basic Science Research.

Selected recent publications:

  1. Bernardo, J., Long, H.J., Simons, E.R.: Early phagosomal responses of human neutrophils to Staphylococcus epidermidis: A novel method for measuring multiple simultaneous events that characterize the early phagosomal environment. Cytometry A 2010; 77:243-252. PMID: 19937952
  2. Powell, R.D. III, Whitworth, W.C., Bernardo, J., Moonan, P.K., Mazurek, G.H.: Unusual Interferon Gamma Measurements with QuantiFERON-TB Gold and QuantiFERON-TB Gold In-Tube Tests. PLoS ONE 6(6):e20061. epub 2010 Jun 8. PMID: 21656663
  3. Bernardo, J., Simons, E.R.: Multiparameter flow cytometric kinetics of phagocyte stimulus responses. Cytometry A 2011. epub 7 Jun 2011. PMID: 21687702
  4. Tschampl, C., Bernardo, J., Garvey, T., Garnick, D.: A Performance Measures Proposal on Testing for Latent Tuberculosis Infection (LTBI) in the Primary Care Sector. The Joint Commission Journal on Quality and Patient Safety. 37:309-316, 2011.
  5. Livingston, K.A., Lobato, M.N., Sosa, L.E., Budnick, G.E., Bernardo, J. and the New England Laboratory Study Group: Mycobacterium tuberculosis testing practices in hospital, commercial and state laboratories in the New England states. Int J Tuberc Lung Dis. 15:1218-1222, 2011.
  6. Kellar, K.L., Gehrke, J., Weis, S.E., Mahmutovic-Mayhew, A., Davila, B., Zajdowicz, M.J., Scarborough, R., LoBue, P.A., Lardizabal, A.A., Daley, C.L., Reves, R.R., Bernardo, J., Campbell, B.H., Whitworth, W.C., Mazurek, G.H.: Multiple Cytokines Are Released When Blood from Patients with Tuberculosis Is Stimulated with Mycobacterium tuberculosis Antigens. PLoS ONE 6(11): e26545. doi:10.1371/journal.pone.0026545. epub 2011, Nov 21.
  7. Han, L.L., Elvin, P., Bernardo, J. Nonclinical selection criteria for maximizing the yield of nucleic acid amplification tests in tuberculosis diagnosis. J Clin Microbiol 50: 2592-2595. 2012. epub 2012, May 23.
  8. Mancuso, J.D., Bernardo, J., Mazurek, G.H.: The Elusive “Gold” Standard for Detecting Mycobacterium tuberculosis Infection. Editorial. Amer J Respir Crit Care Med. 187: 122-124, 2013. PMID: 23322793
  9. Marks, S.M., Cronin, W., Venkatappa, T., Maltas, G., Chon, S., Sharnprapai, S., Gaeddert, M., Tapia, J., Dorman, S.E., Etkind, S., Crosby, C., Blumberg, H.M., Bernardo, J.: The Health-System Benefits and Cost-effectiveness of Using Mycobacterium Tuberculosis Direct Nucleic Acid Amplification Testing to Diagnose Tuberculosis Disease in the United States. Clin Infect Dis. 57:532-542, 2013. PMID 23697743
  10. Chiswick, E.L., Mella, J.R., Bernardo, J., Remick, D.G. Acute-Phase Deaths from Murine Polymicrobial Sepsis Are Characterized by Innate Immune Suppression Rather Than Exhaustion. J Immunol. 195:3793-3802, 2015. PMID 26371253cit336 57:532-542, 2013. PMID 23697743 
  11. Schoenberg, N.C., Barker, A.F., Bernardo, J., Deterding, R.R., Ellner, J.J., Hess, D.R., MacIntyre, N.R., Martinez, F.J., Wilson, K.J. A Comparative Analysis of Pulmonary and Critical Care Medicine Guideline Development Methodologies Amer J Respir Crit Care Med. 196:621-627, 2017.

Dr Bernardo see patients in the following locations:

Allergy Clinic, Shapiro 9