Barbara Seaton, Ph.D.
Professor of Physiology and Biophysics
B.S. City University of New York
Ph.D. Massachusetts Institute of Technology
The long-term research interests of our lab concern interactions between mammalian proteins and biomembranes or other large assemblies such as viruses. Our current focus is on the lung surfactant proteins A and D (SP-A and SP-D) and their role in innate defense against inhaled pathogens such as seasonal and pandemic strains of influenza A virus (IAV). These proteins are C-type lectins that can recognize sugars and other signature components on microbial surfaces, and can initiate events leading to pathogen neutralization. In our lab, we use x-ray crystallography, molecular modeling, and various biochemical and biophysical methods to understand the nature of these interactions at the highest resolution possible.
van Eijk M, Rynkiewicz MJ, White MR, Hartshorn KL, Zou X, Schulten K, Luo D, Crouch EC, Cafarella TR, Head JF, Haagsman HP, Seaton BA. (2012) A unique sugar-binding site mediates the distinct anti-influenza activity of pig surfactant protein D. J Biol Chem 287(32), 26666-77. (PMID: 22685299)
Shang FF, Rynkiewicz MJ, McCormack FX, Wu H, Cafarella TM, Head JF, Seaton BA (2011) Crystallographic complexes of surfactant protein-A and carbohydrates reveal ligand-induced conformational change. J. Biol. Chem. 286, 757-65. (PMID: 21047777)
van Eijk, M, Brunsma L, Hartshorn KL, White MR, Rynkiewicz MJ, Seaton BA, Hemrika W, Romijn RA, van Balkom BW, Haagsman HP (2011) Introduction of N-linked glycans in the lectin domain of surfactant protein D: impact on interactions with influenza A viruses. J Biol Chem 286, 20137-51. (PMID:21489996)
Crouch E, Nikolaidis N, McCormack F, McDonald B, Allen K, Rynkiewicz M, Cafarella T, White M, Lewnard K, Leymarie N, Zaia J, Seaton B, Hartshorn K (2011) Mutagenesis of SP-D informed by evolution and x-ray crystallography enhances defenses against influenza in vivo. J. Biol Chem. 286, 40681-92. (PMID: 21965658)
Seaton BA, Crouch EC, McCormack FX, Head JF, Hartshorn KL, Mendelsohn R. (2010) Structural determinants of pattern recognition by lung collectins. Innate Immun. 16: 143-150.
Crouch, E., K. Hartshorn, T. Horlacher, B. McDonald, K. Smith, T. Cafarella, B. Seaton, P. H. Seeberger, and J. Head (2009) Recognition of mannosylated ligands and influenza A virus by human surfactant protein D: contributions of an extended site and residue 343. Biochemistry 48:3335-3345
Wang H, Head J, Kosma P, Brade H, Müller-Loennies S, Sheikh S, McDonald B, Smith K, Cafarella T, Seaton B, Crou.ch E (2008) Recognition of heptoses and the inner core of bacterial lipopolysaccharides by Surfactant Protein D. Biochemistry 47,710-720.
Wang L, Brauner JW, Mao G, Crouch E, Seaton B, Head J, Smith K, Flach CR, Mendelsohn R. (2008) Interaction of recombinant surfactant protein D with lipopolysaccharide: conformation and orientation of bound protein by IRRAS and simulations. Biochemistry 47, 8103-8113.
Crouch, E.C., McDonald, B., Smith, K., Mealy (Carafella), T., Seaton, B.A., Head, J.F. (2007) Critical Role of Arg/Lys343 in the Species-Dependent Recognition of Phosphatidylinositol by Pulmonary Surfactant Protein D. Biochemistry 46, 5160-5169.
Crouch, E.C., McDonald, B., Smith, K., (Mealy) Cafarella, T., Seaton, B.A., Head, J.F. (2006) Contributions of Phe335 to Carbohydrate Recognition by Human Surfactant Protein D: Stacking Interactions with Lung Collectin Ligands. J. Biol. Chem. 281, 18008-14.
Crouch, E.C., Smith, K , McDonald, B., Briner, D., Linders, B., McDonald, J., Holmskov, U., Head, J.F., Hartshorn, K. (2006) Species differences in the carbohydrate binding preferences of surfactant protein D. Am J Respir Cell Mol Biol. 35, 84-94.
Head, J.F., Mealy, T.R., McCormack, F.X., and Seaton, B.A. (2003) Crystal Structure of Trimeric Carbohydrate Recognition and Neck Domains of Surfactant Protein A. J. Biol. Chem. 278, 43254-60.
Department of Physiology and Biophysics
Boston University School of Medicine
72 East Concord Street
Boston MA 02118-2526
Fax: (617) 638-4273