Jeffrey R. Moore, Ph.D.
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Associate Professor Ph.D. University of Vermont Phone: (617) 638-4251 Visit Moore LaboratoryResearchOur lab’s main interest is the study of molecular mechanics and cellular motility. We employ single molecule fluorescence techniques and laser trapping assays to study: the mechanism and regulation muscle contraction, cell motility, and the regulation of cell shape. Our lab’s investigations of cardiac muscle contraction focus on the effects of cardiomyopathy-causing amino acid substitutions on myosin’s mechanical and enzymatic properties. We are also investigating the nature and regulation of cytoskeletal dynamics in differentiated vascular smooth muscle cells. PublicationsJensen M.H., Morris E.J., Huang R., Rebowski G., Dominguez R., Weitz D.A., Moore J.R., Wang C-L. (2012) The conformational state of actin filaments regulates branching by actin-related protein 2/3 (Arp2/3) complex. J Biol Chem. Jul 12. [Epub ahead of print] Moore, J. R., Leinwand, L., Warshaw, D. W. (2012) Understanding Cardiomyopathy Phenotypes Based on the Functional Impact of Mutations in the Myosin Motor. Circulation Res. 20;111:375-85. Jensen M.H., Watt J., Hodgkinson J., Gallant C., Appel S., El-Mezgueldi M., Angelini T., Morgan K.G., Lehman W., and Moore J.R. (2011) Effects of basic calponin on the flexural mechanics and stability of F-actin Cytoskeleton Cytoskeleton 69(1):49-58. Moore, J.R., Nirody J., Li X.E., Fischer S., Lehman W. (2011) Structural implications of conserved aspartate residues located in tropomyosin’s coiled-coil core. BioArchitecture 1:250-255. Collins A., Huang R., Jensen M. H., Moore J.R., Lehman W., Wang Chih-Lueh Albert (2011) Structural studies on maturing actin filaments. BioArchitechture. BioArchitechture. 1:127-33 Lu X., Kazmierczak K., Jiang X., Jones M., Watt J., Helfman D., Moore J.R., Szczesna-Cordary D, Lossos D. (2011) Germinal Center Specific Protein HGAL Directly Interacts with Both Myosin and Actin and Increases the Binding of Myosin to Actin FEBS J. 11:1922-31 Lin T, Greenberg MJ, Moore JR, Ostap EM. (2011) A Hearing Loss-Associated myo1c Mutation (R156W) Decreases the Myosin Duty Ratio and Force Sensitivity. Biochemistry. 50(11):1831-8. Pub Med Greenberg, M. J., Kazmierczak K., Szczesna-Cordary D., Moore J. R. (2010) Cardiomyopathy-linked Myosin Regulatory Light Chain Mutations Disrupt Myosin Strain-Dependent Biochemistry. Proc Natl Acad Sci U S A. 107(40):17403-8. Pub Med Greenberg MJ, Moore JR. (2010) The molecular basis of frictional loads in the in vitro motility assay with applications to the study of the loaded mechanochemistry of molecular motors. Cytoskeleton. 67(5):273-85. Pub Med Greenberg, M., Mealy, T., Jones, M., Szczesna-Cordary, D., Moore, J., (2010) The direct molecular effects of fatigue and myosin regulatory light chain phosphorylation on the actomyosin contractile apparatus. Am J Physiol Regul Integr Comp Physiol 298(4):R989-96. Pub Med Greenberg, M. and Moore, J. (2010) Actin in action and inaction: The differential effects of hypertrophic and dilated cardiomyopathy actin mutations on thin filament J Mol Cell Cardiol. 48:277-8. Pub Med Greenberg, M., Watt, J., Mealy, T., Jones, M., Szczesna-Cordary, D., Moore, J., (2009) The Molecular Effects of Skeletal Muscle Myosin Regulatory Light Chain Phosphorylation Am J Physiol Regul Integr Comp Physiol 297(2): 265-74. Epub 2009 May 20. Pub Med Pant, K., Watt, J., Greenberg, M., Jones, M., Szczesna-Cordary, D., Moore, J., (2009) Removal of the Cardiac Myosin Regulatory Light Chain Increases Isometric Force Production. FASEB J 23: 3571-80. Epub 2009 May 26. Pub Med Greenberg, M.J., Watt J.D., Jones, M., Kazmierczak, K., Szczesna-Cordary, D., Moore, J.,R., (2009).Regulatory light chain mutations associated with cardiomyopathy affect myosin mechanicsand kinetics. J Mol Cell Cardiol. Jan;46(1):108-15. Pub Med Greenberg, M., Wang, C-L., Lehman, W., Moore, J., (2008) Modulation of actin mechanics by caldesmon and tropomyosin. Cell Motility and Cytoskeleton. Feb;65(2):156-64. Pub Med Szczesna-Cordary, D., Jones, M., Moore, J., Watt, J., Kerrick, G., Xu, Y., Wang, Y., Wagg, C., and Lopaschuk, G. (2007) Myosin Regulatory Light Chain E22K Mutation Results in Decreased Cardiac Intracellular Calcium and Force Transients. FASEB J. Dec;21(14):3974-85. Debold, E., Schmitt, J., Moore, J., Patlak, J., Beck, S., Seidman, J., Seidman, C., Warshaw, D. (2007) Hypertrophic and dilated cardiomyopathy mutations differentially affect the molecular force generation of mouse a-cardiac myosin in the laser trap assay. Am J Physiol Heart Circ Physiol. Mar 9; Jul;293(1):H284-91 Moore, J., (2005) Stretch activation: Toward a molecular mechanism. in “Nature’s Versatile engine: Insect flight muscle Inside and Out”, Vigoreaux J., ed., Landes Bioscience Publishers. Moore, J., Krementsova, E., Trybus, K., Warshaw, D.,(2004) Is the myosin V neck a compliant lever?, J. Mus. Res. Cell Motil. 25(1):29-35. Irving T, Bhattacharya S, Tesic I, Moore, J., Farman, Simcox A, Vigoreaux J, Maughan D. (2001) Changes in myofibrillar structure and function produced by N-terminal deletion of the regulatory light chain in Drosophila. Submitted to J. Mus. Res. Cell Motil., 22(8):675-83. Moore, J., Krementsova E, Trybus K, Warshaw D. (2001) Myosin V exhibits a high duty cycle and large unitary displacement, J. Cell Biol.,155 (4): 625-635 . Moore, J., Dickinson M, Vigoreaux J, Maughan D. (2000) The Effect of removing the N-terminal extension of the Drosophila Myosin Regulatory Light Chain Upon Flight Ability and the Contractile Dynamics of Indirect Flight Muscle. Biophys J. 78: 1431-1440. Vigoreaux J, Moore, J., Maughan D. (2000) Role of the elastic protein projectin in stretch activation and work output of Drosophila flight muscles. in “Elastic Filaments of the Cell”, Pollack G. and Granzier H, eds., Kluwer Academic/Plenum Publishers Moore, J., Vigoreaux J, Maughan D. (1999) The Projectin mutant bentD/+ has reduced stretch activation and altered indirect flight muscle kinetics.J. Mus. Res. Cell Motil. 20: 797-806. Maughan D, Moore, J., Vigoreaux J, Barnes B, Mulieri LA. (1998) Work production and work absorption in muscle strips from vertebrate cardiac and insect flight muscle fibers. Adv Exp Med Biol., 453:471-80 Vigoreaux JO, Hernandez C, Moore, J., Ayer G, Maughan D. (1998) A genetic deficiency that spans the flightin gene of Drosophila melanogaster affects the ultrastructure and function of the flight muscles. J Exp Biol., 201 ( Pt 13):2033-44 Dickinson MH, Hyatt CJ, Lehmann FO, Moore, J., Reedy MC, Simcox A, Tohtong R, Vigoreaux JO, Yamashita H, Maughan DW. (1997) Phosphorylation-dependent power output of transgenic flies: an integrated study. Biophys J.. 73(6):3122-34 Book ChaptersMoore, J., (2005) Stretch activation: Toward a molecular mechanism. in “Nature’s Versatile engine: Insect flight muscle Inside and Out”, Vigoreaux J., ed., Landes Bioscience Publishers. Contact UsDepartment of Physiology and Biophysics Phone: (617) 638-4251 |
