15% SDS Gel

15% Separating Gel

Ingredient Volume
dH2O 3.59
1.5M Tris pH 8.8 3.75
30:0.8 Bis-acryl 7.5
TEMED 0.020
10% SDS 0.150

De-Gas, then add

10% Ammonium Persulfate 0.100

5% Stacking Gel

Ingredient Volume
dH2O 1.7
0.5M Tris pH 8.8 0.750
30:0.8 Bis-acryl 0.500
TEMED 0.003
10% SDS 0.030

De-Gas, then add

10% Ammonium Persulfate 0.020

12% Gel

Ingredient Volume
dH2O 4.86
3M Tris pH 8.8 1.19
30:0.8 Bis-acryl 3.9
TEMED 0.005
10% SDS 0.053

De-Gas, then add

10% Ammonium Persulfate 0.050