We Express your Favorite Protein
in Escherichia Coli:
ApoB-5.9 was expressed in E.coli, induced with a final concentration of 1mM IPTG and grown at 37C while shaking at 225 RPM for indicated time periods. Lane 1: size marker in Kb; lane 2: un-induced; lane 3: 2 hours; lane 4: 4 hours; lane 5 overnight
In Spodoptera frugiperda:
Expression and purification of recombinant ApoA-I D(1-59) with Baculovirus expression system. ApoA-I D(1-59) was expressed in SF9 cells and purified in batch format with Ni-NTA resin, fraction analyzed on 12% SDS-PAGE gel and stained with Coomassie blue. Lane1: Total extract. Lane 2: Nonbinding fraction. Lanes 3-5: Wash fractions. Lanes 6-10: Eluted ApoA-I D(1-59). Lane 11: Size marker in Kb.
Or other mammalian cells:
Shown is an autoradiogram of ApoB-32.5 immunoprecipiates from cell lysates (c) and media (m) from 8 separate clones. Clones are selected by amount of ApoB-32.5 protein present. The results clearly show variable expression and secretion of individual clones.