DNA was extracted from transformed colonies, digested with XhoI/NdeI, separated on 1% agarose gel, stained with EtBr and photographed through filter on transilluminator. Panel a): lane1: 1kb ladder, lane14: lHindIII, lanes 2 and 13 positive controls, lanes 3-12 candidates with lanes 3,6,7,8,10 positive for insert. Panel b: lane1: lHindIII ; lane14: 1kb ladder, lanes 3-12: undigested DNA loaded in same orientation as panel a.