Boston University Medical Campus
Mass Spectrometry Resource

Peter B. O’Connor, Curriculum Vitae

Research Interests: Mass Spectrometry instrumentation and applications are fascinating. The intricate coupling of advanced physics techniques, instrumentation, electronics, and software with chemical and particularly biochemical problems of a previously intractable nature is THE reason that I am a mass spectrometrist. My personal interests lie on the instrumentation side of this field, but no one can deny the tremendous growth in exciting mass spectrometry applications, particularly in the proteomics field.

The Fourier Transform Mass Spectrometer is superior to all other mass spectrometers in virtually all important aspects, although niche applications will always exist for other instruments. However, the electronics and software lag relative to other commercial instrumentation due to the lack of sufficient investment by instrument companies in their development. Additionally, most if not all of the fundamental performance parameters we are familiar with in FTMS are very conservative. For example, physicists measuring single atoms in Penning traps (an FTMS is fundamentally a Penning trap) are able to achieve 0.1 ppb mass accuracies where we struggle to achieve <1 ppm. There is much room for improvement.

Current FTMS research projects include:
Fundamentals:
1) Applications of Electron Capture Dissociation.
2) High Pressure MALDI-FTMS for the analysis of labile biomolecules.
3) Design and testing of a large area, high throughput MALDI-FTMS sample target stage that will allow spatially resolved acquisition.
4) Improvement in transfer efficiency of FTMS injection optics.
5) Development of the Boston University Data Analysis (B.U.D.A.) code to assist the huge task of interpreting complex FTMS data on a human timescale.
6) Increasing the m/z range of MALDI-FTMS
7) Development of improved detection electronics for MALDI-FTMS
8) Design of a cryogenic FTMS for increased field, sensitivity, and pumping.

Selected Applications:
1) Detailed oxidative post translational modification mapping of cardiovascular proteins.
2) Detailed phosphorylation site mapping of the transcription related protien RRN3.
3) Study of deamidation and isoaspartic acid formation in the rPA protien used for the next-generation anthrax vaccine
4) Study of deamidation and isoaspartic acid formation in monoclonal antibodies.
5) Isoaspartomic study of yeast with the isoaspartic acid repair enzyme PIMT knocked in/out.
6) Study of deamidation in Cytochrome B5.
7) Study of degradation enzymes for Abeta.

Students Advised:

Graduate students:
Parminder Kaur, ECE, PhD thesis defended Dec 7, 2006.
Vera B. Ivleva, Chemistry, PhD thesis defended May 2007.
Bo Xie, Chemistry, PhD thesis defended May 2007.
Raman Mathur, ECE, PhD thesis defended March 2008.
Jason Cournoyer, Chemistry, PhD thesis defended April 2008.
Konstantine Aizikov, ECE, spring 2009.
Xiaojuan Li, Biochemistry, spring 2009.
Nadezhda Sargaeva, Biochemistry, spring 2010.
Terry Lin, ECE, spring 2012.
Xiang Yu, Biochemistry, spring 2012.

Undergraduate students:
Eric Fallows, graduated spring 2005.
Marina Belyayev, graduated spring 2006.

Postdoctoral fellows:
Ekaterina Mirgorodskaya, 2002-2004
Nancy Leymarie, 2002-2004
Bogdan Budnik, 2003-2005
Jason Pittman, 2003-2005
Susanne Moyer, 2003-2004
Judith Jebanathirajah, 2004-2005
Cheng Lin, 2005-present
Cheng Zhao, 2005-2007
Chunxiang Yao, 2007-present
Weidong Cui, 2008-present

Professional Positions:

2005-present; Research Associate Professor of Biochemistry, Boston University School of Medicine, Boston, MA
2000-2005; Research Assistant Professor of Biochemistry, Boston University School of Medicine, Boston, MA
2000-present; Assistant Director, Mass Spectrometry Resource, Boston University School of Medicine, Boston, MA
1999-2000; Senior Research Associate, Mass Spectrometry Resource, Boston University School of Medicine, Boston, MA
1997-1999; Scientist, IonSpec Corporation, Irvine, CA
1995-1997; Post Doctoral Researcher, FOM Institute for Atomic and Molecular physics, Amsterdam, The Netherlands
1992-1995; Research Assistant, Department of Chemistry, Cornell University, Ithaca, NY
1991-1992; Teaching Assistant, Department of Chemistry, Cornell University, Ithaca, NY
1990-1991; Teaching Assistant, Department of Chemistry, University of North Texas, Denton, TX
1989-1991; Undergraduate Research Assistant, Department of Chemistry, University of North Texas, Denton, TX
summer 1989; Undergraduate Research Assistant (NSF REU Fellowship), Department of Chemistry, Florida State University, Tallahassee, FL

Education:

1995-1997; Post Doctoral Researcher, Prof. Jaap J. Boon, Dr. Ron M. A. Heeren, FOM Institute for Atomic and Molecular Physics, Amsterdam, The Netherlands
1991-1995; Ph.D., Prof. Fred McLafferty, Chemistry, Cornell University, Ithaca, NY
1991-1993; M.S., Prof. Fred McLafferty, Chemistry, Cornell University, Ithaca, NY
1987-1991; B.S., Chemistry, University of North Texas, Denton, TX

Professional Service:

2008, Session Chair: “Developments in Ion Trap Instrumentation”, American Society for Mass Spectrometry Meeting, Denver
2008, PhD Thesis Committee (Major Advisor), Jason Cournoyer, Boston University Department of Chemistry.
2008, PhD Thesis Committee (Major Advisor), Raman Mathur, Boston University Department of Electrical and Computer Engineering.
2008, Reviewer NIH Special Emphasis Panel.
2008, Reviewer NIH Instrument and Systems Development Study Section.
2008, Reviewer NSF SBIR/STTR panel.
2008, Reviewer NIH SBIR/STTR panel.
2008, Session Chair: “Advanced Proteomic Technologies”, US HUPO meeting
2008; Program Committee, US HUPO meeting
2007; Program Committee, International FTMS meeting, Moscow, Russia
2007; Reviewer NIH Shared Instrument Grant Panel
2007; PhD Thesis Committee, Vera Ivleva, Boston University Department of Chemistry
2007; Reviewer NIH Special Emphasis Panel on Mass Spectrometry Instrumentation
2007; PhD Thesis Committee, Bo Xie, Boston University Department of Chemistry
2007; PhD Thesis Committee, Romulus Mihalca, FOM Institute for Atomic and Molecular Physics and Utrecht University
2007; Editorial Board, Journal of the American Society for Mass Spectrometry
2007; Reviewer NSF SBIR panel
2006; PhD Thesis Committee (Major Advisor), Parminder Kaur, Boston University Department of Electrical and Computer Engineering.
2006; ASMS Sanibel Committee (Chair)
2006; Oxidative Post Translational Modifications of Proteins in Cardiovascular Disease, program committee
2006; Reviewer NIH-High End Instrumentation Shared Instrument Grant program (chairman)
2005-2006; Reviewer NIH-ISD Instrumentation and Systems Development Study Section (permanent member)
2005; ASMS Sanibel Committee (Treasurer)
2005; Session Chair “FTMS Instrumentation and Methods” 53rd ASMS conference, San Antonio, Texas
2004; ASMS Sanibel Committee (member)
2004; Reviewer NIH-ISD Instrumentation and Systems Development Study Section (temporary member)
2004; Reviewer NIH Special Emphasis Panel, “High End Shared Instrument Grant Program”
2004; Oxidative Post Translational Modifications of Proteins in Cardiovascular Disease, program committee
2002; Reviewer, Phillip-Morris Research Program
2002; Reviewer, DOE “Genomes to Life” Grant Program
2002; Reviewer, NIH-BECM “High End Shared Instrument Grant Program”
2002; Session Chair “High Pressure MALDI”, 50th ASMS meeting, Orlando, FL
2002; BUSM Department of Biochemistry Admissions Committee
2001; ASMS program committee
1995-present; Reviewer, Analytical Chemistry
1995-present; Reviewer, Journal of the American Society for Mass Spectrometry
1995-present; Reviewer, International Journal of Mass Spectrometry
1999-present; Reviewer, Environmental Science and Technology
1999-present; Reviewer, Analytical Biochemistry
2000-present; Reviewer, Reviews of Scientific Instruments
2002-present; Reviewer, Journal of the American Chemical Society

Teaching:

Spring Semester 2008: GMS_BI_793 Mass Spectrometry, Proteomics, and Functional Genomics (4, 2-hour lectures)
Spring Semester 2007: GMS_BI_793 Mass Spectrometry, Proteomics, and Functional Genomics (4, 2-hour lectures)
Spring Semester 2006: GMS_BI_793 Mass Spectrometry, Proteomics, and Functional Genomics (4, 2-hour lectures)
Fall Semester 2005: GMS_BI_793 Mass Spectrometry, Proteomics, and Functional Genomics (4, 2-hour lectures)
Spring Semester 2005: GMS_BI_793 Mass Spectrometry, Proteomics, and Functional Genomics (4, 2-hour lectures)
Spring Semester 2004: GMS_BI_793 Mass Spectrometry, Proteomics, and Functional Genomics (4, 2-hour lectures)
Spring Semester 2003: GMS_BI_793 Mass Spectrometry, Proteomics, and Functional Genomics (4, 2-hour lectures)
Spring Semester 2002: GMS_BI_793 Mass Spectrometry, Proteomics, and Functional Genomics (3, 2-hour lectures)

Departmental and University Committees:

2003-2007; BU Physics Department, Scientific Instrument Facility Advisory Committee
2003-2005; BU School of Medicine, Department of Biochemistry Seminar Committee (chairman)
2002-2005; BU School of Medicine, Department of Biochemistry Seminar Committee
2001-2003; BU School of Medicine, Department of Biochemistry Graduate Admissions and Recruitment Committee

Honors:

1991; Graduated Magna Cum Laude, University of North Texas, Denton, TX
1990; Master’s Award, Department of Chemistry, University of North Texas, Denton, TX
1989; Addie Mae Curbo Lloyd Award, Department of Chemistry, University of North Texas, Denton, TX
1987-1991; Robert A. Welch Foundation Scholarship
1987; University Interscholastic League Science Competition, Division A, 5th in Texas State competition.

Professional Associations:

American Society for Mass Spectrometry
American Association for the Advancement of Science
American Chemical Society
American Physical Society
Alpha Chi Sigma professional chemistry fraternity

Funding:

Pending:
NIH/NCRR-R01, “Proteomic analysis using MAF”, P.I. Peter B. O’Connor, ~$1.8M (direct,) 2007-2012. Note: the second submission for this proposal scored 2.9th percentile. It should be funded, depending on program decisions.

Current:
NIH/NIGMS-R01, “Defining the Isoaspartome”, P.I. Peter B. O’Connor, ~$1M (direct), 2007-2012.
NIH-NCRR-P41, “Mass Spectrometry Resource for Biology and Medicine”, P.I. Cathy Costello, ~$5.5M (direct), FTMS portion ~$1.5M (direct), 2007-2012.
NIH/NHLBI-N01, “Cardiovascular Proteomics Center”, P.I. Cathy Costello and Richard Cohen, ~$8M (direct), FTMS portion ~$2M (direct), 2002-2009.

Completed:
MDS-Sciex, “ESI-qQq-FTMS”, P.I. Peter B. O’Connor, $150k + ~$50k parts (direct), 2003-2006.
ACS-Petroleum Research Fund, “Which ECD fragments are products of a free radical cascade?”, P.I. Peter B. O’Connor, $120k (direct), 2003-2006.
NIH/NCRR-P41, “Mass Spectrometry Resource for Biology and Medicine”, P.I. Cathy Costello, ~$5.5M (direct), FTMS portion ~$1.5M (direct), 2002-2007.

Patents:

1. O’Connor, P. B. Apparatus and Method for Ion Cyclotron Resonance Mass Spectrometry USA 2003 10/338423

Publications (reverse chronological order):

68. Aizikov, K.; Mathur, R.; O’Connor, P. B. The spontaneous loss of coherence catastrophe in Fourier transform ion cyclotron resonance mass spectrometry 2008, submitted.

67. Mathur, R.; Knepper, R. W.; O’Connor, P. B. A low noise, broadband cryogenic preamplifier operated in a high field superconducting magnet IEEE Trans. on App. Supercond. 2008, in press.

66. Li, X.; Cournoyer, J. C.; Lin, C.; O’Connor, P. B. The effect of fixed charge modifications on electron capture dissociation J. Am. Soc. Mass Spectrom. 2008, in press. Available online 28 June 2008.

65. Li, X.; Cournoyer, J. C.; Lin, C.; O’Connor, P. B. Use of 18O labels to monitor deamidation during protein and peptide sample processing J. Am. Soc. Mass Spectrom. 2008, 19, 855-864.

64. Cournoyer, J. C.; Lin, C.; O’Connor, P. B. Gas-phase structure dissimilarity of protonated isomeric peptides revealed by IR-activated DR-ECD J. Am. Soc. Mass Spectrom. 2008, in revision.

63. Lin, C.; Cournoyer, J. C.; O’Connor, P. B. Probing the gas phase folding kinetics of peptide ions by IR-activated DR-ECD J. Am. Soc. Mass Spectrom. 2008, 19, 780-789.

62. Xie, B.; Luo, X.; Zhao, C.; Priest, C. M.; Chan, S.-Y.; O’Connor, P. B.; Kirschner, D. A.; Costello, C. E. Molecular characterization of myelin protein zero in xenopus laevis peripheral nerve: Equilibrium between non-covalently associated dimer and monomer Int. J. Mass Spectrom. Ion Processes 2007, 268, 304-315 .

61. Lin, C.; Mathur, R.; Aizikov, K.; O’Connor, P. B. First signal on the cryogenic Fourier-transform ion cyclotron resonance mass spectrometer J. Am. Soc. Mass Spectrom. 2007, 18, 2090-2093 Link.

60. Mathur, R.; Knepper, R. W.; O’Connor, P. B. A low-noise, wideband preamplifier for a Fourier-transform ion cyclotron resonance mass spectrometer J. Am. Soc. Mass Spectrom. 2007, 18, 2233-2241 Link.

59. Zhao, C.; Cournoyer, J.; Xie, B.; Zaia, J.; Costello, C. E.; O’Connor, P. B. Structural analysis of milk oligosaccharides and N-linked branched glycans by electron detachment dissociation 2007, in revision.

58. Zhao, C.; Xie, B.; Chan, S.-Y.; Costello, C. E.; O’Connor, P. B. Collisionally activated dissociation and electron capture dissociation provide complementary structural information for permethylated oligosaccharides J. Am. Soc. Mass Spectrom. 2007, 19, 138-150 Link.

57. Zhao, C.; O’Connor, P. B. Removal of polyethylene glycols from protein samples using titanium dioxide Anal. Biochem. 2007, 365, 283-285 .

56. Cournoyer, J. J.; O’Connor, P. B.  Analysis of deamidation in proteins in Protein Mass Spectrometry; Whitelegge, J. P., Ed., 2008, Elsevier, Amsterdam.

55. O’Connor, P. B. The development of Matrix-assisted laser desorption ion sources In Electrospray ionization mass spectrometry; Cole, R. B., Ed.; 2007 Wiley Interscience, San Francisco.

54. O’Connor, P. B.; Hillenkamp, F. MALDI mass spectrometry insturmentation In MALDI-MS: a practical guide to instrumentation, methods and applications; Hillenkamp, F., Peter-Katalinic, J., Eds.; 2007, Wiley-VCH Verlag Weinheim, Germany.

53. Kaur, P.; O’Connor, P. B. Quantitative determination of isotope ratios from experimental isotopic distributions Anal. Chem. 2007, 79, 1198-1204 Link.

52. Mathur, R.; O’Connor, P. B. Design and implementation of a high power RF oscillator on a printed circuit board for multipole ion guides Rev. Sci. Instrum. 2006, 77, 114101 Link.

51. Kaur, P.; O’Connor, P. B. Comparison of charge state determination methods for high resolution mass spectra IEEE Int. Conf. on Granular Computing May 10, 2006, 550-553 .

50. Cournoyer, J. C.; Lin, C.; Bowman, M. J.; O’Connor, P. B. Quantitating the relative abundance of isoaspartyl residues in deamidated proteins by electron capture dissociation J. Am. Soc. Mass Spectrom. 2007, 18, 48-56. Link.

49. Belyayev, M. A.; Cournoyer, J. J.; Lin, C.; O’Connor, P. B. The effect of radical trap moieties on electron capture dissociation spectra of substance P J. Am. Soc. Mass Spectrom. 2006, 17, 1428-1436 Link.

48. Lin, C.; Cournoyer, J. J.; O’Connor, P. B. Use of a double resonance electron capture dissociation experiment to probe fragment intermediate lifetimes J. Am. Soc. Mass Spectrom. 2006, 17, 1605-1615. Link.

47. Zhao, C.; Sethuraman, M.; Clavreul, N.; Kaur, P.; Cohen, R. A.; O’Connor, P. B. A detailed map of oxidative post-translational modifications of human p21ras using Fourier transform mass spectrometry Anal. Chem. 2006, 78, 5134-5142. Link.

46. Aizikov, K.; O’Connor, P. B. Use of the filter diagonalization method in the study of space charge related frequency modulation in FTMS J. Am. Soc. Mass Spectrom. 2006, 17, 836-843 Link.

45. O’Connor, P. B.; Cournoyer, J. J.; Pitteri, S. J.; Chrisman, P. A.; McLuckey, S. A. Differentiation of aspartic and isoaspartic acids using electron transfer dissociation J. Am. Soc. Mass Spectrom. 2006, 17, 15-19 Link.

44. Perlman, D. H.; Berg, E. A.; O’Connor, P. B.; Costello, C. E.; Hu, J. Reverse transcription-associated dephosphorylation of hepadnavirus nucleocapsids Proc. Nat. Acad. Sci. USA 2005, 102, 9020-9025 Link.

43. O’Connor, P. B.; Lin, C.; Cournoyer, J. J.; Pittman, J. L.; Belyayev, M.; Budnik, B. A. Long-lived electron capture dissociation product ions experience radical migration via hydrogen abstraction J. Am. Soc. Mass Spectrom. 2006, 17, 576-585 Link.

42. Kaur, P.; O’Connor, P. B. Algorithms for automatic interpretation of high resolution mass spectra J. Am. Soc. Mass Spectrom. 2006, 17, 459-468 Link.

41. Cournoyer, J. J.; Lin, C.; O’Connor, P. B. Detecting deamidation products in proteins by electron capture dissociation Anal. Chem. 2006, 78, 1264-1271 .Link.

40. O’Connor, P. B.; Pittman, J. L.; Thomson, B. A.; Budnik, B. A.; Cournoyer, J. C.; Jebanathirajah, J.; Lin, C.; Moyer, S.; Zhao, C. A new hybrid electrospray Fourier transform mass spectrometer: Design and performance characteristics Rapid Commun Mass Spectrom 2006, 20, 259-266.

39. Jebanathirajah, J. A.; Pittman, J. L.; Thomson, B. A.; Budnik, B. A.; Kaur, P.; Rape, M.; Kirschner, M.; Costello, C. E.; O’Connor, P. B. Characterization of a new qQq-FTICR mass spectrometer for post-translational modification analysis and top-down tandem mass spectrometry of whole proteins J. Am. Soc. Mass Spectrom. 2005, 16, 1985-1999 Link.

38. Ivleva, V. B.; Sapp, L. M.; O’Connor, P. B.; Costello, C. E. Ganglioside analysis by thin-layer chromatography matrix-assisted laser desorption/ionization orthogonal time-of-flight mass spectrometry J. Am. Soc. Mass Spectrom. 2005, 16, 1552-1560 Link.

37. Cournoyer, J. J.; Pittman, J. L.; Ivleva, V. B.; Fallows, E.; Waskell, L.; Costello, C. E.; O’Connor, P. B. Deamidation: Differentiation of aspartyl from isoaspartyl products in peptides by electron capture dissociation Protein Science 2005, 14, 452-463.

36. Pittman, J. L.; O’Connor, P. B. A minimum thickness gate valve with integrated ion optics for mass spectrometry J. Am. Soc. Mass Spectrom. 2005, 16, 441-445 Link.

35. Moody, D. B.; Young, D. C.; Cheng, T.-Y.; Rostat, J.-P.; Roura-mir, C.; O’Connor, P. B.; Zajonc, D. M.; Walz, A.; Miller, M. J.; Levery, S. B.; Wilson, I. A.; Costello, C. E.; Brenner, M. B. T-cell activation by lipopeptide antigens Science 2004, 203, 527-531. Link

34. Kaur, P.; O’Connor, P. B. Use of statistical methods for quantitative determination of the number of trapped ions Anal. Chem. 2003, 76, 2756-2762 Link.

33. Budnik, B. A.; Moyer, S. C.; Pittman, J. L.; Ivleva, V. B.; Sommer, U.; Costello, C. E.; O’Connor, P. B. High pressure MALDI-FTMS: Implications for proteomics Int. J. Mass Spectrom. Ion Processes 2004, 234, 203-212.

32. Ivleva, V. B.; Elkin, Y. N.; Budnik, B. A.; Moyer, S. C.; O’Connor, P. B.; Costello, C. E. Coupling thin layer chromatography with high pressure matrix assisted laser desorption/ionization Fourier transform mass spectrometry for the analysis of ganglioside mixtures Anal. Chem. 2004, 76, 6484-6491 Link.

31. O’Connor, P. B.; Budnik, B. A.; Ivleva, V. B.; Kaur, P.; Moyer, S. C.; Pittman, J. L.; Costello, C. E. A High pressure matrix-assisted laser desorption Fourier-transform mass spectrometry ion source designed to accommodate large targets with diverse surfaces J Am Soc Mass Spectrom 2003, 15, 128-132.

30. Moyer, S. C.; Budnik, B. A.; Pittman, J. L.; Costello, C. E.; O’Connor, P. B. Attomole peptide analysis by high-pressure matrix-assisted laser desorption/ionization Fourier transform mass spectrometry Anal. Chem. 2003, 75, 6449-6454. Link.

29. Leymarie, N.; Costello, C. E.; O’Connor, P. B. Electron capture dissociation initiates a free radical reaction cascade J. Am. Chem. Soc. 2002, 125, 8949-8958. Link

28. O’Connor, P. B.; Costello, C. A.; Earle, W. E. A high voltage RF oscillator for driving multipole ion guides J. Am. Soc. Mass Spectrom. 2002, 13, 1370-1375. Link.

27.  Leymarie, N.; Berg, E. A.; McComb, M. E.; O’Connor, P. B.; Grogan, J.; Oppenheim, F. G.; Costello, C. E. Tandem Mass Spectrometry for Structural Characterization of Proline-Rich Proteins: Application to Salivary PRP-3 Anal. Chem. 2002, 74, 4124-4132. Link.

26. Hopkins, C. E.; O’Connor P, B.; Allen, K. N.; Costello, C. E.; Tolan, D. R. Chemical-modification rescue assessed by mass spectrometry demonstrates that γ-thia-lysine yields the same activity as lysine in aldolase Protein Sci 2002, 11, 1591-1599. Link

25. McClellan, J. E.; Costello, C. E.; O’Connor, P. B.; Zaia, J. Influence of charge state on product ion mass spectra and the determination of 4s/6s sulfation sequence of chondroitin sulfate oligosaccharides Anal. Chem. 2002, 74, 3760-3771 Link.

24. O’Connor, P. B. Considerations for the design of a Fourier-transform mass spectrometer in the 4.2K cold bore of a superconducting magnet Rapid Commun Mass Spectrom 2002, 16, 1160-1167. Link

23. Mirgorodskaya, E.; O’Connor, P. B.; Costello, C. E. A general method for precalculation of parameters for sustained off resonance irradiation/collision-induced dissociation J. Am. Soc. Mass Spectrom. 2002, 13, 318-324 Link.

22. O’Connor, P. B.; Mirgorodskaya, E.; Costello, C. E. High pressure matrix-assisted laser desorption/ionization Fourier-transform mass spectrometry for minimization of ganglioside fragmentation J. Am. Soc. Mass Spectrom. 2002, 13, 402-407 Link.

21. Lim, A.; Prokaeva, T.; McComb, M. E.; O’Connor, P. B.; Theberge, R.; Connors, L. H.; Skinner, M.; Costello, C. E. Characterization of transthyretin variants in familial transthyretin amyloidosis by mass spectrometric peptide mapping and DNA sequence analysis Anal. Chem. 2002, 74, 741-751 Link.

20. Costello, C. E.; Lim, A.; O’Connor, P. B.; Prokaeva, T.; Connors, L. H.; Skinner, M. Identification of novel transthyretin variants in familial transthyretin amyloidosis by mass spectrometric peptide mapping Amyloid 2001, 8, 104-105.

19. O’Connor, P. B.; Costello, C. E. A high pressure matrix-assisted laser desorption/ionization Fourier-transform mass spectrometry ion source for thermal stabilization of labile biomolecules Rapid Commun. Mass Spectrom. 2001, 15, 1862-1868.

18. van den Brink, O. F.; Boon, J. J.; O’Connor, P. B.; Duursma, M. C.; Heeren, R. M. A. Matrix-assisted laser desorption/ionization Fourier transform mass spectrometric analysis of oxygenated triglycerides and phosphatidylcholines in egg tempera paint dosimeters used for environmental monitoring of museum display conditions J. Mass Spectrom. 2001, 36, 479-492.

17. O’Connor, P. B.; Costello, C. E. Application of multishot acquisition in Fourier transform mass spectrometry Anal. Chem. 2000, 72, 5125-5130 Link.

16. O’Connor, P. B.; Costello, C. E. Internal calibration on adjacent samples (INCAS) with Fourier-transform mass spectrometry Anal. Chem. 2000, 72, 5881-5885 Link.

15. O’Connor, P. B.; Duursma, M. C.; Rooij, G. J. v.; Heeren, R. M. A.; Boon, J. J. Correction of time of flight shifted polymeric molecular weight distributions in MALDI-FTMS Anal. Chem. 1997, 69, 2751-2755 Link.

14. Guan, Z.; Kelleher, N. L.; O’Connor, P. B.; Aaserud, D. J.; Little, D. P.; McLafferty, F. W. 193 nm photodissociation of larger multiply charged biomolecules Int. J. Mass Spectrom. Ion Processes 1996, 157/158, 357-364.

13. Moertz, E.; O’Connor, P. B.; Roepstorff, P.; Kelleher, N. L.; Wood, T. D.; McLafferty, F. W.; Mann, M. Sequence tag identification of intact proteins by matching tandem mass spectral data against sequence databases Proc. Nat. Acad. Sci. USA 1996, 93, 8264-8267.

12. O’Connor, P. B.; McLafferty, F. W. Oligomer characterizationof 4-22 kDa polymers by electrospray Fourier-transform mass spectrometry J. Am. Chem. Soc. 1995, 117, 12826-12831 Link.

11. O’Connor, P. B.; Speir, J. P.; Wood, T. D.; Chorush, R. A.; Guan, Z.; McLafferty, F. W. Quadrupolar axialization of large multiply charged ions J. Mass Spectrom. 1996, 31, 555-559.

10. Aaserud, D. J.; Little, D. P.; O’Connor, P. B.; McLafferty, F. W. Distinguishing N- and C- terminus ions for mass spectrometry sequencing of proteins without prior degradation Rapid Commun. Mass Spectrom. 1995, 9, 871-876.

9. Kelleher, N. L.; Senko, M. W.; Little, D. P.; O’Connor, P. B.; McLafferty, F. W. Complete large-molecule high-resolution mass spectra from 50-femtomole microvolume injection J. Am. Soc. Mass Spectrom. 1995, 6, 220-221.

8. McLafferty, F. W.; Senko, M. W.; Little, D. P.; Wood, T. D.; O’Connor, P. B.; Speir, J. P.; Chorush, R. A.; Kelleher, N. L. Electrospray ionization and Fourier-transfrom mass spectrometry in biomedical research Adv. Mass Spectrom. 1995, 13, 115-128.

7. O’Connor, P. B.; Little, D. P.; McLafferty, F. W. Isotopic assignment in large molecule mass spectra by fragmentation of a selected isotopic peak Anal. Chem. 1996, 68, 542-545 Link.

6. O’Connor, P. B.; McLafferty, F. W. High resolution ion isolation with the capacitively coupled open cell J. Am. Soc. Mass Spectrom. 1995, 6, 533-535.

5. O’Connor, P. B.; Speir, J. P.; Senko, M. W.; Little, D. P.; McLafferty, F. W. Tandem mass spectrometry of carbonic anhydrase (29 kDa) J. Mass Spectrom. 1995, 30, 88-93.

4. Wood, T. D.; Chorush, R. A.; Wampler, F. M. I.; Little, D. P.; O’Connor, P. B.; McLafferty, F. W. Gas phase folding and unfolding of cytochrome-c cations Proc. Nat. Acad. Sci. USA 1995, 92, 2451.

3. Little, D. P.; Speir, J. P.; Senko, M. W.; O’Connor, P. B.; McLafferty, F. W. Infrared multiphoton dissociation of large multiply-charged ions for biomolecule sequencing Anal. Chem. 1994, 66, 2809-2815.

2. Marshall, P.; O’Connor, P. B.; Chan, W.-T.; Kristof, P. B.; Goddard, J. D. In Gas Phase Metal Reactions; Fontijn, A., Ed.; Elsevier: Amsterdam, 1992.

1. Cioslowski, J.; O’Connor, P. B.; Fleischmann, E. D. Is Superbenzene Superaromatic? J. Am. Chem. Soc. 1991, 113, 1086.

Colloquia, Invited Talks, etc:

April, 2008, Coventry, UK, University of Warwick Department of Chemistry, “Fourier Transform Ion Cyclotron Resonance Mass Spectrometry in the Post-Genomic World” (interview lecture)
March, 2008, PACMASS Discussion Group, Seattle, “Fourier Transform Ion Cyclotron Resonance Mass Spectrometry in the Post-Genomic World” (invited lecture)
March, 2008, University of Washington, Seattle, “Cryogenic Fourier Transform Mass Spectrometry: How and Why” (invited lecture)
March, 2008, Pittcon, “Cryogenic Fourier Transform Mass Spectrometry (invited lecture)
January, 2008, Clemson University, “Fourier Transform Ion Cyclotron Resonance Mass Spectrometry in the Post-Genomic World” (interview lecture)
December 2007, Paris, France, 5th Uppsala meeting on ECD (invited lecture)
November 2007, Geneva, Switzerland, “Discussions on the Mechanism of ECD” (invited lecture)
November 2007, University of Illinois Chicago “Fourier Transform Ion Cyclotron Resonance Mass Spectrometry in the Post-Genomic World” (interview lecture)
November 2007, University of Maryland – College Park, “Fourier Transform Ion Cyclotron Resonance Mass Spectrometry: Research Plan” (interview lecture)
November 2007, University of Maryland – College Park, “Fourier Transform Ion Cyclotron Resonance Mass Spectrometry: Instrumentation and Applications” (interview lecture)
November 2007, University of Texas San Antonio, “Fourier Transform Ion Cyclotron Resonance Mass Spectrometry: Combining Engineering, Chemistry, and Biomedical Research for Fun and Profit” (interview lecture)
November 2007, University of Arkansas, Fayetteville, “Fourier Transform Ion Cyclotron Resonance Mass Spectrometry: Instrumentation and Applications” (invited lecture)
September 2007, NHLBI Proteomics Centers PI Meeting, Bethesda, Maryland, “The State of Mass Spectrometry Instrumentation for Proteomics.”
September 2007, Greater Boston Mass Spectrometry Discussion Group, Bruker Daltonics, Billerica, MA, “Cryogenic FTMS”
August 2007, North Carolina Central University, Biomanufacturing Research Institute and Technology Enterprise, “Fourier Transform Ion Cyclotron Resonance Mass Spectrometry: Improving Performance and Extending Applications” (invited lecture)
July 2007, University of Texas San Antonio, Department of Biology, “Fourier Transform Ion Cyclotron Resonance Mass Spectrometry: Improving Performance and Extending Applications” (interview lecture)
June 2007, Indianapolis, ASMS meeting FTMS workshop, “Cryogenic FTMS: First results”
May 2007, Canadian Pharmaceutical & Biomedical Analysis Symposium, Montreal, Canada, “Deamidation and isoaspartic acid analysis using ECD” (invited lecture)
May 2007, Virginia Bioinformatics Institute, Virginia Tech, “Fourier Transform Ion Cyclotron Resonance Mass Spectrometry: Improving Performance and Extending Applications” (interview lecture)
May 2007, University of Nebraska – Lincoln, Department of Chemistry, “Fourier Transform Ion Cyclotron Resonance Mass Spectrometry: Improving Performance and Extending Applications” (interview lecture)
April 2007, North American FTMS meeting, Lake Tahoe, CA, “Cryogenic FTMS” (invited lecture)
Feb 2007, Korean Institute for Science and Standards, Daejeon, South Korea “Deamidation and isoaspartic acid analysis using ECD” (invited lecture)
Dec 2006, Weisinger Health Center, Danville PA, “Post-translational modification analysis using high performance FTICRMS” (invited lecture)
Dec 2006, Hong Kong, China, 4th Uppsala meeting on ECD, “Deamidation and isoaspartic acid analysis using ECD” (invited lecture)
Nov 2006, Beatenberg, Switzerland, Swiss Mass Spectrometry Society Meeting (SGMS) “On the role of free radical rearrangements in ECD” (invited lecture)
Oct 2006, Boston, MA, OPTM workshop “FTMS and Proteomics” (invited lecture)
Sept 2006, Athens, Greece, “Cryogenic FTMS” (invited lecture)
Apr 2006; Agilent, Santa Clara, CA, “Deamidation and Isomerization of Amino Acids in Proteins”
Dec 2005; 3rd Uppsala meeting on ECD, Seattle, “On the role of free radical rearrangments in ECD”
Oct 2005; FACCS meeting Quebec “Cryogenic FTMS”
August 2005; 7th International Workshop on Mass Spectrometry in Biology and Medicine “Deamidation and Isomerization of Amino Acids in Proteins”
June 2005; 53rd ASMS Conference, San Antonio, Texas “Deamidation and Isomerization of Amino Acids in Proteins”
Oct. 2004; MGH Sympsium, Boston, MA, “Fundamentals of Electrospray Ionization”
Sept. 2004; Desorption 2004 conference, St. Petersburgh, Russia (Invited Talk) “Cryogenic FTMS Design”
Sept. 2004; 2nd Upsala conference on ECD, Edinburgh, Scotland (Invited Talk) “Distinguishing isomers of aspartic acid by ECD, and new results on the ECD mechanism”
August, 2004; Cornell University Department of Chemistry, “Distinguishing isomers of aspartic acid by ECD, and new results on the ECD mechanism”
March, 30, 2004; 7th European FTMS Workshop, Konstanz, Germany (Invited Talk) “Complete Design of the Cryogenic FTMS”
February, 6, 2004; MDS Sciex, Toronto, Canada (Invited Talk) “A new ESI qQq-FTMS: Design and performance”
November, 6, 2003; Uppsala conference on Electron Capture Dissociation and Related topics; Stockholm, Sweden (Invited Talk) “Electron Capture Dissociation initiates a Free Radical Cascade”
August, 18 2003; Institute for Systems Biology, Seattle Washington (Departmental Colloquium) “Advantages and Challenges in the design and application of MALDI-Cryogenic FTMS for Proteomics”
August, 19 2003; University of Washinton, Department of Chemistry, Seattle Washington (Invited Talk) “Electron Capture Dissociation Initiates a Free Radical Reaction Cascade”
August, 20 2003; Oregon State University, Department of Chemistry, Corvallis, OR (Departmental Colloquium) “Advantages and Challenges in the design and application of MALDI-Cryogenic FTMS for Proteomics”
April 17, 2003; Boston University, Boston, MA (Electrical and Computer Engineering Departmental Seminar) “Advantages and Challenges in the design and application of MALDI-Cryogenic FTMS for Proteomics”
March 11, 2003; NIH NHLBI Proteomics Center PI Meeting, Washington DC “Advantages and Challenges in the design and application of MALDI-Cryogenic FTMS for Proteomics”
Feb 22, 2003; BU School of Medicine, Boston, MA (Cardiovascular Proteomics Center invited talk) “Advantages and Challenges in the design and application of MALDI-Cryogenic FTMS for Proteomics”
Dec. 12, 2002; Boston University, Boston, MA (Biomedical Engineering Departmental Seminar)”Advantages and Challenges in the design and application of MALDI-Cryogenic FTMS for Proteomics”
Dec. 5, 2002; East Tennessee Mass Spectrometry Discussion Group Meeting, Knoxville, TN (invited talk)”Advantages and Challenges in the design and application of MALDI-Cryogenic FTMS for Proteomics”
Dec. 5, 2002; Oak Ridge National Lab, Oak Ridge, TN (Analytical Division Seminar) “Electron Capture Dissociation of Cyclic Peptides”
Nov. 22, 2002; University of Maine, Orono, ME (Chemistry Departmental Seminar) “Advantages and Challenges in the design and application of MALDI-Cryogenic FTMS for Proteomics”
Nov. 7, 2002; FOM-AMOLF Institute, Amsterdam (invited talk)
“Electron Capture Dissociation of Cyclic Peptides”
Nov. 7, 2002; FOM-AMOLF Institute, Amsterdam (invited talk)
“Advantages and Challenges in the design and application of MALDI-Cryogenic FTMS for Proteomics”
Nov. 4, 2002; Max Planck Institute, Berlin (invited talk)
“Advantages and Challenges in the design and application of MALDI-Cryogenic FTMS for Proteomics”
Oct. 31, 2002; Cambridge University, UK, (invited talk)
“Advantages and Challenges in the design and application of MALDI-Cryogenic FTMS for Proteomics”
Oct. 9, 2002; National High Magnetic Field Lab, Tallahassee FL (Invited Talk) “Electron Capture Dissociation of Cyclic Peptides”
Sept. 4, 2002; Desorption 2002, Estes Park, CO (invited talk) “High Pressure MALDI-FTMS”
Aug. 23, 2002; MDS Sciex, Toronto, CA (invited talk) “Fourier Transform Mass Spectrometry: New Developments and Commercial Prospects”
Oct. 2001; 6th European FTMS Meeting (invited talk) “High Pressure MALDI-FTMS”
Nov. 2001; Northeastern University (Chemistry Departmental Seminar) “High Pressure MALDI-FTMS”
July 2001; Northeast Section American Chemical Society (invited talk)
June 2001; 49th ASMS, Chicago, IL (invited talk)
March 2001; North American FTICR meeting, Austin, TX (invited talk)
Spring 2000; Boston University School of Medicine (Biochemistry Colloquium)
Summer 1999; BU Mass Spectrometry Resource (invited talk)
Spring 1999; American Chemical Society meeting, San Francisco, CA (invited talk)
June 1998; 46th ASMS, Orlando, Fl (invited talk)
Fall, 1997; Merck Research Institute, NJ (invited talk)
March 1997; North American FTICR Meeting, Tallahassee, FL (invited talk)
March 1997; Schering-Plough Research Institute, NJ (invited talk)
June 1995; 44th ASMS (invited talk)