Proteoglycans and glycosaminoglycans

Glycosaminoglycans (GAGs) are present on the surfaces of all adherent animal cells and in extracellular matrices. GAGs interact with many families of growth factors and growth factor receptors and thus influence cell growth and differentiation.

Click here for a primer on GAG structure, biology, and mass spectrometry.

The Mass Spectrometry Resource is developing methods for analysis of glycosaminoglycans and proteoglycans from tissue biospecimens.160711 PG gordon JZ

Here are some publications related to this project:

  • Thelin, M.; Svensson, K. J.; Shi, X.; Bagher, M.; Axelsson, J.; Isinger-Ekstrand, A.; van Kuppevelt, T. H.; Johansson, J.; Nilbert, M.; Zaia, J.; Belting, M.; Maccarana, M.; Malmstrom, A. Dermatan sulfate is involved in the tumorigenic properties of Esophagus Squamous Cell Carcinoma. Cancer Res. 2012, 72, 1943-1952. Pubmed Link
  • Shao, C.; Shi, X.; Phillips, J. J.; Zaia, J. Mass spectral profiling of glycosaminoglycans from histological tissue surfaces. Anal. Chem. 2013, 85, 10984-10991. Pubmed Link
  • Turi├ík, L.; Shao, C.; Meng, L.; Khatri, K.; Leymarie, N.; Wang, Q.; Pantazopoulos, H.; Leon, D. R.; Zaia, J. Workflow for Combined Proteomics and Glycomics Profiling from Histological Tissues. Anal. Chem. 2014, 86, 9670-9678. Pubmed Link
  • Huang, Y.; Mao, Y.; Zong, C.; Lin, C.; Boons, G.; Zaia, J. Discovery of a Heparan Sulfate 3-O-Sulfation Specific Peeling Reaction. Anal. Chem. 2015, 81, 592-600. Pubmed Link
  • Tykesson, E.; Mao, Y.; Maccarana, M.; Pu, Y.; Gao, J.; Lin, C.; Zaia, J.; Westergren-Thorsson, G.; Ellervik, U.; Malmstrom, L.; Malmstrom, A. Deciphering the mode of action of the processive polysaccharide modifying enzyme dermatan sulfate epimerase 1 by hydrogen-deuterium exchange mass spectrometry. Chemical Science 2016, 7, 1447-1456. Pubmed Link
  • Zaia, J.; Khatri, K.; Klein, J. A.; Shao, C.; Sheng, Y.; Viner, R. Complete Molecular Weight Profiling of Low Molecular Weight Heparins Using Size Exclusion Chromatography-Ion Suppressor-High Resolution Mass Spectrometry. Anal. Chem. 2016, 88, 10654-10660. Pubmed Link