Ion Torrent Semiconductor Next-Generation Sequencing

Ion Semiconductor Sequencing is a method of next-generation sequencing based on the detection of hydrogen ions that are released during the polymerization of DNA. This is a method of sequencing by synthesis, during which a complementary strand is built based on the sequence of a template strand.  This technology differs from other sequencing technologies in that no modified nucleotides or optics are used.  At present, supported applications for Ion Torrent semiconductor sequencing include: targeted DNA and RNA sequencing; exome sequencing; transcriptome sequencing; small RNA Sequencing; gene expression profiling; microbial or other small genome sequencing; as well as bacterial and viral typing.  In contrast to other sequencing platforms, Ion Torrent semiconductor sequencing has the advantages of quicker sequencing runs and reduced cost per sample.  Ion Torrent semiconductor sequencing is performed on either the Personal Genome Machine (PGM) or Proton instrument depending on desired read length and application.

Ion Torrent Certified Service Provider

The BU Microarray & Sequencing Resource is a Certified Service Provider for Ion AmpliSeq Transcriptome sequencing.  The Ion Torrent Certified Service Provider program is a global network of validated service providers that enable researchers in small and medium-sized laboratories to get high-quality transcriptome sequencing data generated on the Ion Proton instrument.  

Ion Torrent PGM Sequencing

The Ion Torrent Personal Genome Machine (PGM) is a small-scale, bench top instrument capable of processing samples for targeted DNA and RNA sequencing, small RNA sequencing, gene expression profiling, microbial genome sequencing, and bacterial and viral typing applications.  The PGM can provide three levels of sequence output according to three sequencing chip sizes: 314, 316, and 318.  The PGM can also provide sequence data in two read-lengths: 200bp and 400bp.  See below for detailed information about output by chip type.  For questions regarding PGM applications and choosing those best suited to your experimental needs, please contact the Microarray & Sequencing Resource.

Chip Type Sequencing Time Output [Mb] Output [Reads]
314 2.5 hrs 60 – 120 400-700K
316 3.0 hrs 300 – 600 2.0-3.5M
318 4.5 hrs 475 – 950 3.0-5.0M

The above data are provided for 200bp read-length applications and are dependent on the quality of input RNA and DNA, as well as final sequencing libraries.

Ion Torrent Proton Sequencing

The Ion Torrent Proton sequencer is a larger-scale instrument than the Personal Genome Machine, and is capable of processing samples for all applications supported by the PGM in addition to whole transcriptome and exome sequencing.  Currently, the Proton Instrument utilizes the P1 chip to provide 4-7 Gb of sequencing over 60-80M reads of approximately 150bp length in 4.5 hours, dependent on the quality of input RNA and DNA, as well as final sequencing libraries.  For questions regarding Proton applications and choosing those best suited to your experimental needs, please contact the Microarray & Sequencing Resource.