Protocols

For centralized access to downloadable protocols, all our protocols are available at the protocols webpage of the Boston University Center for Regenerative Medicine (CReM).


Finn Hawkins first green cells

New! Human Lung Directed Differentiation Protocol FH 2017. From Hawkins et al JCI 2017

New! Hawkins excel file with reagent catalogue numbers and handy Reagent calculator (JCI 2017).

NEED MORE HANDS-ON TRAINING? Attend our annual 1 week hands-0n training course on lung directed differentiation, held every spring in the CReM. Sign up through the CReM website.

Please click here to visit the CReM protocols page, where you’ll find all our protocols related to:

  1. Directed Differentiation of mouse or human pluripotent stem cells into lung, thyroid, or liver lineages
  2. Reprogramming and Derivation of Induced Pluripotent Stem (iPS) Cells
  3. Lentiviral packaging and titering
  4. Culture Maintenance and Passaging of undifferentiated Embryonic Stem Cells or iPS Cells

If you can’t find the protocol you are looking for, please ask and we’ll send it to you! Click ‘Contact Us’ and ask our Lab Manager.