COVID-19: Requesting our cells

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Organoids

We currently have iAEC2 spheres and iBC spheres in culture, and can prepare shipments as live cells. If you and your lab are interested in receiving these cells from the CReM, please have the PI or group leader send an email to Darrell Kotton and CC our Lab Manager, Olivia, and our senior post-doc, Jessie, at:

dkotton at bu.edu; ohix at bu.edu; hjess at bu.edu

Please include the following information in your email:

  • “Requesting Cells” in the subject line of the email
  • Full name
  • Contact Information (tel)
  • Requested cell type
  • Shipping Address
  • Fedex Account Number
  • Purpose of experiment

After you send us your request, we will contact you regarding reagents and protocols necessary for continued expansion and culture of our cells, as well as with details for the invoicing of the requested cell lines.

 

To prepare to receive our cells, you can freely download all our detailed protocols needed for culturing them here. Of these, the most relevant protocols for receiving, passaging, differentiating, and maintaining iAEC2s are:

  1. Derivation of self-renewing lung alveolar epithelial type II cells from human pluripotent stem cells (Nature Protocols)
  2. Receiving Shipped iAEC2s
  3. Derivation of Epithelial-Only Airway Organoids from Human Pluripotent Stem Cells
  4. Human ESC/iPSC airway differentiation (bronchospheres)
  5. Maintenance of iPSC-derived alveolospheres
  6. CD47/CD26 staining for FACS enrichment of human ESC/iPSC derived NKX2-1+ lung progenitors

If you would like to make a donation to financially support our cell sharing mission, click here!

 

iPSCs

If you and your lab are interested in receiving vials of undifferentiated iPSCs from the CReM, please see our iPSC bank linked below, and reach out to our iPSC Core manager, Marianne James, PhD at:

mfjames at bu.edu

CReM iPSC bank: https://stemcellbank.bu.edu/Catalog/Item/Home